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The map label for this gene is nikB [C]

Identifier: 157370982

GI number: 157370982

Start: 3020644

End: 3022362

Strand: Direct

Name: nikB [C]

Synonym: Spro_2742

Alternate gene names: 157370982

Gene position: 3020644-3022362 (Clockwise)

Preceding gene: 157370981

Following gene: 157370983

Centisome position: 55.44

GC content: 63.06

Gene sequence:

>1719_bases
ATGCGACTGGCGCAATGGCTATTGCCACTGGCCTCGCGGCTGTTTGCCCTGTTGGCAATGATATCCCTGATCGGTCTGAT
GCCCTGGCTATCCGGTCGCGACCCGGCAATTAGCCTGCTGCGAGCTCGCTCGGCCGAACAGGAAGCGACGCCCGAAGCCC
TGAATGCCATTCGCCAACGCTTTGCTCTGGACGACGGGCCGCTGATGCTGCTTGGCCGCTGGCTTAATGGGGTATTGCAT
GGCGACGCCGGTCACTCCTGGATCTCCGGTCGCCCGGTACTGGAGGGGCTGTTACAGGCTACCGGGGTTTCTCTAACGTT
AATGGGCTACGCGCTGGCGATAGCACTGTTAGTCGCAGGGTTGCTGGCACTGCCAACCCTTTGGCGCGGCCTGCATGGCG
AGTACCGCCGTTCTGCAGGCGTGTTGGCGGTGGCGCTGACCGCCCTGCCCGAGTTTCTCCTGGCCGCGCTGTTACTGATC
CTCGGCGCCGTCTGGCTAAACTGGTTTCCCCCCTACGGCTGGCAAAGTTTGCGCCATGTGGTGCTGCCGGCGCTGGCATT
GGGTTTACCTGCCGGCGGCCTGCTGGGCCTGTTGTTCAGTGACGGTCTGGCCGCGACCTTATCCGAACGCTGGTTAATCA
CCTGGAGCCTGGCCGGTATCTCCCGGTGGCATTGTCTGCTGGCGGTGTTGCGCCGCACGCTACCCGCCTTGTTACCGCAA
ATCGGTTTGGTGCTGGTTGGGCTTACCGGCGGCGCCATCGCCGTCGAGAAAGTCTTCGCCATTCCCGGTCTGGGCAGAGC
GACATTAGGTGCCGCCATCGCACAAGATCTGCCAACGCTGCAGTGCGGCATTCTGATCTTGTTGCTGATCGCCTTCAGCA
CCGGTTTTTTGGCACAGGGCGCGCATTATCTGTTGCTGGGTCGGGTAGCGCCAAGCCTGGCCAATTCGATGCCGCCACCG
CACAGCGGCAAAGGCAGTTACGTTTTCGCCACCTTTGCCGCGCTGTTGTTGTGCTTGTTGGTGCTGAGCGGGATCATACG
CGATCCACTTTCGTCTCTGCATATTCGCCTCCAGCCGCCCAGCCTCGATCTGCCCTTCGGCGCGGATGCCACCGGTCGGG
ACATCCTGGCGCGCGTCGCTTACGGTGCGCTGAATACCAGCCTGCTGGCACTGGCGGTCAGCTTCGCTTCGCTGTTCCTC
GGGATAATGTTCGGGCTGATGCCACGTCTGATGTGCGGAATGATTGAGCTGGCCAACGCCACACCGCCGATTATCGCAGG
CCTGTTAGTGGTCGCCATTAGCGGCCCTTCAGCCTACGGCGCCGCCTGCGCGGTGATGTTGGTCAGTTGGGCTCCGCTGG
CCGCTCATTGCGCTTCGCTGCTGATGGAAGCCCGCACTCAGCCCTATATCCGCATGCTGCCGGTGTTGGGGATTGGTCGA
CTGCGCACCTTGACGCATTACCTGCTGCCTGCCTTATGGGGGCCACTGCTGCGCCATGCCATGCTGCGCCTGCCGGGGAT
TGCGCTGGCGCTGGCCGCCCTGGGTTTCCTTGGCCTGGGTCCGCAGCCACCCAGCGCAGAATGGGGGCTGGTGCTGGCGG
AAGGCATGCCTTATATCGAACGGGCGCCGTGGGGAGTCCTGGCACCGGCGATCGCTCTGATATTACTGGCGGTGCTCGCG
GTAACGTTGGCCAATACCGCCGGCAACCGTCGGGGCTGA

Upstream 100 bases:

>100_bases
TGATCCAGGGGGAAGCCGTGGGCATGAAGAATGCAGAGCGCGATCCGCGAGAACGCCAACTGGTGACACCGCGCAGCATG
ATCGTCAGCGGACAGCCCTG

Downstream 100 bases:

>100_bases
CGGCGCATCCTGGCCCGGTTTGTAGTAATATTATGCGGTACCTCAACCGGCCGGGAGACCCAATGCACCATCTGATGTTA
GATATTGAAACGCTGGATAT

Product: binding-protein-dependent transport systems inner membrane component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRLAQWLLPLASRLFALLAMISLIGLMPWLSGRDPAISLLRARSAEQEATPEALNAIRQRFALDDGPLMLLGRWLNGVLH
GDAGHSWISGRPVLEGLLQATGVSLTLMGYALAIALLVAGLLALPTLWRGLHGEYRRSAGVLAVALTALPEFLLAALLLI
LGAVWLNWFPPYGWQSLRHVVLPALALGLPAGGLLGLLFSDGLAATLSERWLITWSLAGISRWHCLLAVLRRTLPALLPQ
IGLVLVGLTGGAIAVEKVFAIPGLGRATLGAAIAQDLPTLQCGILILLLIAFSTGFLAQGAHYLLLGRVAPSLANSMPPP
HSGKGSYVFATFAALLLCLLVLSGIIRDPLSSLHIRLQPPSLDLPFGADATGRDILARVAYGALNTSLLALAVSFASLFL
GIMFGLMPRLMCGMIELANATPPIIAGLLVVAISGPSAYGAACAVMLVSWAPLAAHCASLLMEARTQPYIRMLPVLGIGR
LRTLTHYLLPALWGPLLRHAMLRLPGIALALAALGFLGLGPQPPSAEWGLVLAEGMPYIERAPWGVLAPAIALILLAVLA
VTLANTAGNRRG

Sequences:

>Translated_572_residues
MRLAQWLLPLASRLFALLAMISLIGLMPWLSGRDPAISLLRARSAEQEATPEALNAIRQRFALDDGPLMLLGRWLNGVLH
GDAGHSWISGRPVLEGLLQATGVSLTLMGYALAIALLVAGLLALPTLWRGLHGEYRRSAGVLAVALTALPEFLLAALLLI
LGAVWLNWFPPYGWQSLRHVVLPALALGLPAGGLLGLLFSDGLAATLSERWLITWSLAGISRWHCLLAVLRRTLPALLPQ
IGLVLVGLTGGAIAVEKVFAIPGLGRATLGAAIAQDLPTLQCGILILLLIAFSTGFLAQGAHYLLLGRVAPSLANSMPPP
HSGKGSYVFATFAALLLCLLVLSGIIRDPLSSLHIRLQPPSLDLPFGADATGRDILARVAYGALNTSLLALAVSFASLFL
GIMFGLMPRLMCGMIELANATPPIIAGLLVVAISGPSAYGAACAVMLVSWAPLAAHCASLLMEARTQPYIRMLPVLGIGR
LRTLTHYLLPALWGPLLRHAMLRLPGIALALAALGFLGLGPQPPSAEWGLVLAEGMPYIERAPWGVLAPAIALILLAVLA
VTLANTAGNRRG
>Mature_572_residues
MRLAQWLLPLASRLFALLAMISLIGLMPWLSGRDPAISLLRARSAEQEATPEALNAIRQRFALDDGPLMLLGRWLNGVLH
GDAGHSWISGRPVLEGLLQATGVSLTLMGYALAIALLVAGLLALPTLWRGLHGEYRRSAGVLAVALTALPEFLLAALLLI
LGAVWLNWFPPYGWQSLRHVVLPALALGLPAGGLLGLLFSDGLAATLSERWLITWSLAGISRWHCLLAVLRRTLPALLPQ
IGLVLVGLTGGAIAVEKVFAIPGLGRATLGAAIAQDLPTLQCGILILLLIAFSTGFLAQGAHYLLLGRVAPSLANSMPPP
HSGKGSYVFATFAALLLCLLVLSGIIRDPLSSLHIRLQPPSLDLPFGADATGRDILARVAYGALNTSLLALAVSFASLFL
GIMFGLMPRLMCGMIELANATPPIIAGLLVVAISGPSAYGAACAVMLVSWAPLAAHCASLLMEARTQPYIRMLPVLGIGR
LRTLTHYLLPALWGPLLRHAMLRLPGIALALAALGFLGLGPQPPSAEWGLVLAEGMPYIERAPWGVLAPAIALILLAVLA
VTLANTAGNRRG

Specific function: Probably part of an ABC transporter complex that could be involved in peptide import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=282, Percent_Identity=30.4964539007092, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787053, Length=247, Percent_Identity=29.9595141700405, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787760, Length=249, Percent_Identity=28.1124497991968, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789964, Length=205, Percent_Identity=33.1707317073171, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787549, Length=233, Percent_Identity=31.3304721030043, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787054, Length=212, Percent_Identity=30.188679245283, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1787498, Length=195, Percent_Identity=28.7179487179487, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787497, Length=245, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787550, Length=250, Percent_Identity=25.6, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789889, Length=220, Percent_Identity=26.8181818181818, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 60263; Mature: 60263

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAQWLLPLASRLFALLAMISLIGLMPWLSGRDPAISLLRARSAEQEATPEALNAIRQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
FALDDGPLMLLGRWLNGVLHGDAGHSWISGRPVLEGLLQATGVSLTLMGYALAIALLVAG
HCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LLALPTLWRGLHGEYRRSAGVLAVALTALPEFLLAALLLILGAVWLNWFPPYGWQSLRHV
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VLPALALGLPAGGLLGLLFSDGLAATLSERWLITWSLAGISRWHCLLAVLRRTLPALLPQ
HHHHHHHCCCCHHHHHHHHHCCHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IGLVLVGLTGGAIAVEKVFAIPGLGRATLGAAIAQDLPTLQCGILILLLIAFSTGFLAQG
HHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCC
AHYLLLGRVAPSLANSMPPPHSGKGSYVFATFAALLLCLLVLSGIIRDPLSSLHIRLQPP
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEECCC
SLDLPFGADATGRDILARVAYGALNTSLLALAVSFASLFLGIMFGLMPRLMCGMIELANA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPPIIAGLLVVAISGPSAYGAACAVMLVSWAPLAAHCASLLMEARTQPYIRMLPVLGIGR
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHH
LRTLTHYLLPALWGPLLRHAMLRLPGIALALAALGFLGLGPQPPSAEWGLVLAEGMPYIE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHH
RAPWGVLAPAIALILLAVLAVTLANTAGNRRG
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRLAQWLLPLASRLFALLAMISLIGLMPWLSGRDPAISLLRARSAEQEATPEALNAIRQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
FALDDGPLMLLGRWLNGVLHGDAGHSWISGRPVLEGLLQATGVSLTLMGYALAIALLVAG
HCCCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LLALPTLWRGLHGEYRRSAGVLAVALTALPEFLLAALLLILGAVWLNWFPPYGWQSLRHV
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VLPALALGLPAGGLLGLLFSDGLAATLSERWLITWSLAGISRWHCLLAVLRRTLPALLPQ
HHHHHHHCCCCHHHHHHHHHCCHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IGLVLVGLTGGAIAVEKVFAIPGLGRATLGAAIAQDLPTLQCGILILLLIAFSTGFLAQG
HHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCC
AHYLLLGRVAPSLANSMPPPHSGKGSYVFATFAALLLCLLVLSGIIRDPLSSLHIRLQPP
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEECCC
SLDLPFGADATGRDILARVAYGALNTSLLALAVSFASLFLGIMFGLMPRLMCGMIELANA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPPIIAGLLVVAISGPSAYGAACAVMLVSWAPLAAHCASLLMEARTQPYIRMLPVLGIGR
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHH
LRTLTHYLLPALWGPLLRHAMLRLPGIALALAALGFLGLGPQPPSAEWGLVLAEGMPYIE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHH
RAPWGVLAPAIALILLAVLAVTLANTAGNRRG
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]