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The map label for this gene is pncA [H]

Identifier: 157370964

GI number: 157370964

Start: 3001383

End: 3002015

Strand: Direct

Name: pncA [H]

Synonym: Spro_2724

Alternate gene names: 157370964

Gene position: 3001383-3002015 (Clockwise)

Preceding gene: 157370963

Following gene: 157370965

Centisome position: 55.08

GC content: 57.03

Gene sequence:

>633_bases
ATGAAAACAGCCCTGTTGCTTATCGATTTGCAAAACGACTTTTGCCCCGGCGGCGCACTGGCTGTGACCAACGGTGATGC
CGTCATCAGCGTTGCCAATCAGGCCATAGCGGCCTGCAGCGCACGCGGTGAGCCAGTAGTCGCCTGCCAGGACTGGCATC
CCGCCAATCACCGCAGCTTTGCGGTAAATGCCAACGCCCCCGTGGGCACTCTGGGTGAGCTGGAGGGGTTGCCACAGGTT
TGGTGGCCGGTGCATTGCGTACAGGAAAGCCAGGGCGCTGAGTTTCATCCGCAATTACACCAACGGGACATTATCGCGGT
GTTCCGTAAAGGGCAAAATACGGATATCGACAGCTACAGCGCCTTCTTCGACAACGGTCACCGCTCGCACACCGAGTTGG
ACAGCTGGCTAAAAACGCAGAACGTTAGCCATCTGGCGATTATGGGGTTAGCAACCGACTACTGCGTAAAATTCAGCGTG
CTGGATGCGCTGGAGTTGGGCTACAGAACTACGGTGATTACCGATGGCTGCCGCGGGGTTGATTTGCAGCCCGGCGACAG
TCAGGAAGCCCTGCGGATAATGCAACAGGCCGGAGCGCAATTAGTTACCCTGTCACAATTCATTGACCGCTGA

Upstream 100 bases:

>100_bases
GAGCCAACCGCTGACGCCGGAACAAATTCGCAGCCAGATGCAACTAGATCTGCGTGGCGAGTTAAGCATTAGCGGTTAAC
CCATACACCACGGAGGCGCA

Downstream 100 bases:

>100_bases
TGCCATTGGGCGCTTTTTGCGCCCTTTTCTCCCCGGCCCAAATCTCGACATCGCTCACATTCCGACCATTCTCCTGTGAT
TACATCTTGTTCGCGTGCGG

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKTALLLIDLQNDFCPGGALAVTNGDAVISVANQAIAACSARGEPVVACQDWHPANHRSFAVNANAPVGTLGELEGLPQV
WWPVHCVQESQGAEFHPQLHQRDIIAVFRKGQNTDIDSYSAFFDNGHRSHTELDSWLKTQNVSHLAIMGLATDYCVKFSV
LDALELGYRTTVITDGCRGVDLQPGDSQEALRIMQQAGAQLVTLSQFIDR

Sequences:

>Translated_210_residues
MKTALLLIDLQNDFCPGGALAVTNGDAVISVANQAIAACSARGEPVVACQDWHPANHRSFAVNANAPVGTLGELEGLPQV
WWPVHCVQESQGAEFHPQLHQRDIIAVFRKGQNTDIDSYSAFFDNGHRSHTELDSWLKTQNVSHLAIMGLATDYCVKFSV
LDALELGYRTTVITDGCRGVDLQPGDSQEALRIMQQAGAQLVTLSQFIDR
>Mature_210_residues
MKTALLLIDLQNDFCPGGALAVTNGDAVISVANQAIAACSARGEPVVACQDWHPANHRSFAVNANAPVGTLGELEGLPQV
WWPVHCVQESQGAEFHPQLHQRDIIAVFRKGQNTDIDSYSAFFDNGHRSHTELDSWLKTQNVSHLAIMGLATDYCVKFSV
LDALELGYRTTVITDGCRGVDLQPGDSQEALRIMQQAGAQLVTLSQFIDR

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=204, Percent_Identity=61.2745098039216, Blast_Score=263, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI17543130, Length=203, Percent_Identity=35.4679802955665, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI193206735, Length=203, Percent_Identity=35.4679802955665, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71999339, Length=233, Percent_Identity=29.1845493562232, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321401, Length=197, Percent_Identity=33.502538071066, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648247, Length=204, Percent_Identity=34.8039215686275, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22856; Mature: 22856

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALLLIDLQNDFCPGGALAVTNGDAVISVANQAIAACSARGEPVVACQDWHPANHRSF
CCEEEEEEECCCCCCCCCEEEEECCCEEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCEE
AVNANAPVGTLGELEGLPQVWWPVHCVQESQGAEFHPQLHQRDIIAVFRKGQNTDIDSYS
EEECCCCCCCCHHHCCCCCHHHHHEEEECCCCCCCCCCHHHHHEEEEEECCCCCCCCHHH
AFFDNGHRSHTELDSWLKTQNVSHLAIMGLATDYCVKFSVLDALELGYRTTVITDGCRGV
HHHCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
DLQPGDSQEALRIMQQAGAQLVTLSQFIDR
CCCCCCHHHHHHHHHHCCCCEEEHHHHHCC
>Mature Secondary Structure
MKTALLLIDLQNDFCPGGALAVTNGDAVISVANQAIAACSARGEPVVACQDWHPANHRSF
CCEEEEEEECCCCCCCCCEEEEECCCEEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCEE
AVNANAPVGTLGELEGLPQVWWPVHCVQESQGAEFHPQLHQRDIIAVFRKGQNTDIDSYS
EEECCCCCCCCHHHCCCCCHHHHHEEEECCCCCCCCCCHHHHHEEEEEECCCCCCCCHHH
AFFDNGHRSHTELDSWLKTQNVSHLAIMGLATDYCVKFSVLDALELGYRTTVITDGCRGV
HHHCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
DLQPGDSQEALRIMQQAGAQLVTLSQFIDR
CCCCCCHHHHHHHHHHCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]