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The map label for this gene is ansA [H]

Identifier: 157370963

GI number: 157370963

Start: 3000345

End: 3001361

Strand: Direct

Name: ansA [H]

Synonym: Spro_2723

Alternate gene names: 157370963

Gene position: 3000345-3001361 (Clockwise)

Preceding gene: 157370962

Following gene: 157370964

Centisome position: 55.06

GC content: 57.03

Gene sequence:

>1017_bases
ATGCAAAAGAAATCGATTTACGTCGCCTACACTGGCGGGACCATCGGCATGCAGCGTTCTGAGCACGGCTATATCCCAGT
CTCCGGCCACCTGCAGCGGCAACTGGCCCTGATGCCGGAATTCCACCGGCCGGAAATGCCGGACTTCACCATTCATGAAT
ATGCACCGCTGATCGACTCCTCCGACATGACGCCGGAAGACTGGCAGCATATCGCCGACGATATCAAACTGAATTATGAC
CGTTATGACGGCTTCGTGATCCTGCACGGTACCGACACCATGGCGTTCACCGCCTCGGCGCTGTCATTCATGCTGGAAAA
TCTGGCCAAGCCGGTGATTGTCACCGGTTCTCAGATCCCACTGGCGGAACTGCGTTCCGATGGCCAGACCAACTTGCTGA
ACGCGCTGTATCTGGCAGCCAACCATCCGGTAAATGAAGTCAGCCTGTTCTTCAATAACAAGCTGTTCCGCGGCAACCGG
ACCACCAAGGCGCATGCTGACGGTTTCGACGCCTTTGCTTCACCCAACCTGCCGCCGCTGCTGGAGGCCGGTATTCACAT
TCGCCGCCAGAACGGCATCGACAGCCCGGTCTGCAATGGCGAACTCAAGGTGCATGACATTACGCCGCAGCCGATCGGTG
TGGTGACCATTTACCCAGGCATTTCCGGCGCGGTAGTACGCAACTTCCTGCTACAGCCGGTGAAAGCCTTGATCCTGCGC
TCTTACGGGGTAGGCAACGCGCCGCAAAAAGCAGAGTTGGTGGATGAATTGCGCGACGCTTCCGAACGCGGCATTGTGGT
GGTCAACCTGACGCAGTGCATCTCCGGGAGGGTCAATATGGAAGGCTACGCCACCGGTAATGCACTGGCCCATGCCGGCG
TCATCAGTGGATTTGACATGACGGTCGAGGCCGCATTGACCAAGTTGCATTACCTGTTGAGCCAACCGCTGACGCCGGAA
CAAATTCGCAGCCAGATGCAACTAGATCTGCGTGGCGAGTTAAGCATTAGCGGTTAA

Upstream 100 bases:

>100_bases
CCGCTAACAAATTAATCCTCAGCCCGGCACCCGCCGGGCTTTTTTTCTGCGCTTATCCCCCTATAATTCAGCCCATCTTA
TTTCTGTTAAAACAATCACT

Downstream 100 bases:

>100_bases
CCCATACACCACGGAGGCGCAATGAAAACAGCCCTGTTGCTTATCGATTTGCAAAACGACTTTTGCCCCGGCGGCGCACT
GGCTGTGACCAACGGTGATG

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQKKSIYVAYTGGTIGMQRSEHGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDSSDMTPEDWQHIADDIKLNYD
RYDGFVILHGTDTMAFTASALSFMLENLAKPVIVTGSQIPLAELRSDGQTNLLNALYLAANHPVNEVSLFFNNKLFRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRQNGIDSPVCNGELKVHDITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
SYGVGNAPQKAELVDELRDASERGIVVVNLTQCISGRVNMEGYATGNALAHAGVISGFDMTVEAALTKLHYLLSQPLTPE
QIRSQMQLDLRGELSISG

Sequences:

>Translated_338_residues
MQKKSIYVAYTGGTIGMQRSEHGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDSSDMTPEDWQHIADDIKLNYD
RYDGFVILHGTDTMAFTASALSFMLENLAKPVIVTGSQIPLAELRSDGQTNLLNALYLAANHPVNEVSLFFNNKLFRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRQNGIDSPVCNGELKVHDITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
SYGVGNAPQKAELVDELRDASERGIVVVNLTQCISGRVNMEGYATGNALAHAGVISGFDMTVEAALTKLHYLLSQPLTPE
QIRSQMQLDLRGELSISG
>Mature_338_residues
MQKKSIYVAYTGGTIGMQRSEHGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDSSDMTPEDWQHIADDIKLNYD
RYDGFVILHGTDTMAFTASALSFMLENLAKPVIVTGSQIPLAELRSDGQTNLLNALYLAANHPVNEVSLFFNNKLFRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRQNGIDSPVCNGELKVHDITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
SYGVGNAPQKAELVDELRDASERGIVVVNLTQCISGRVNMEGYATGNALAHAGVISGFDMTVEAALTKLHYLLSQPLTPE
QIRSQMQLDLRGELSISG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=359, Percent_Identity=47.075208913649, Blast_Score=284, Evalue=9e-77,
Organism=Escherichia coli, GI1788065, Length=335, Percent_Identity=83.8805970149254, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1789327, Length=282, Percent_Identity=28.0141843971631, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=363, Percent_Identity=44.0771349862259, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17558278, Length=363, Percent_Identity=44.0771349862259, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI193207374, Length=363, Percent_Identity=44.0771349862259, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17558280, Length=363, Percent_Identity=44.0771349862259, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24639609, Length=364, Percent_Identity=43.956043956044, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24645530, Length=360, Percent_Identity=42.7777777777778, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37059; Mature: 37059

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKSIYVAYTGGTIGMQRSEHGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDS
CCCCEEEEEEECCEEECCCCCCCEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
SDMTPEDWQHIADDIKLNYDRYDGFVILHGTDTMAFTASALSFMLENLAKPVIVTGSQIP
CCCCHHHHHHHHHHHEECEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
LAELRSDGQTNLLNALYLAANHPVNEVSLFFNNKLFRGNRTTKAHADGFDAFASPNLPPL
HHHHHCCCCHHHHHHHHEECCCCHHHEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRQNGIDSPVCNGELKVHDITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
HHCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQKAELVDELRDASERGIVVVNLTQCISGRVNMEGYATGNALAHAGVISGFDM
HCCCCCCCHHHHHHHHHHCHHHCCEEEEEHHHHHCCCCCCCEEECCCHHHHHHECCCCCH
TVEAALTKLHYLLSQPLTPEQIRSQMQLDLRGELSISG
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MQKKSIYVAYTGGTIGMQRSEHGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYAPLIDS
CCCCEEEEEEECCEEECCCCCCCEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
SDMTPEDWQHIADDIKLNYDRYDGFVILHGTDTMAFTASALSFMLENLAKPVIVTGSQIP
CCCCHHHHHHHHHHHEECEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
LAELRSDGQTNLLNALYLAANHPVNEVSLFFNNKLFRGNRTTKAHADGFDAFASPNLPPL
HHHHHCCCCHHHHHHHHEECCCCHHHEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRQNGIDSPVCNGELKVHDITPQPIGVVTIYPGISGAVVRNFLLQPVKALILR
HHCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQKAELVDELRDASERGIVVVNLTQCISGRVNMEGYATGNALAHAGVISGFDM
HCCCCCCCHHHHHHHHHHCHHHCCEEEEEHHHHHCCCCCCCEEECCCHHHHHHECCCCCH
TVEAALTKLHYLLSQPLTPEQIRSQMQLDLRGELSISG
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]