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The map label for this gene is ogt [H]

Identifier: 157370821

GI number: 157370821

Start: 2837786

End: 2838382

Strand: Direct

Name: ogt [H]

Synonym: Spro_2581

Alternate gene names: 157370821

Gene position: 2837786-2838382 (Clockwise)

Preceding gene: 157370820

Following gene: 157370822

Centisome position: 52.08

GC content: 55.78

Gene sequence:

>597_bases
ATGTCCGGAATCCGCCATCGCTCCCCCGCATTTCGTGCAATAATCCCCTCATCAAACGAGAGGAGAGTTCACATGCAGAC
CTTTTTTATTGACCGTATGGCGACGCCGGTAGGCGAACTGGTGCTGATTGCCGATGAGCAGGACCGCTTGCGCGCCATCG
ACTGGACTGAGCATGAAGTACGCCTGATGAAGCTGCTGAATACTCATTACCGAGCCGATCGTTTTCAACTGATAGACAAA
CACAACCCCGGCGGCCTGACCGAAGCGATGCAACGCTATTTTTCGGGTGAATTGGATGTCATTGATCAGTTGCCGGTAAT
GACCGCCGGTACCGAATTCCAGCGCAGCGTCTGGCAGCAACTGCGCCAAATCCCCTGCGGGGAAATCATTACCTATGGCG
AACTGGCGAAACGCATAGGTCGCCCGACCGCTTCACGCGCAGTCGGTATGGCTAACGGCTCCAACCCCATCAGCATTGTG
GTGCCCTGCCACCGGGTGATTGGTTCGCAAGGCGCCTTGACCGGCTATGCCGGTGGCGTTCAGCGCAAGCAGTGGCTGCT
CAAGCACGAAGGGTATTTGCAAAACGATCTGCTTTAA

Upstream 100 bases:

>100_bases
ACTTATTGAAGATTTGTAAATTTATTTTATACATTTGCGGCATAAAGCGACACACCACATTCATGCTCACAGTATGGGCA
ACCCGAGGTTGCCCATCTTC

Downstream 100 bases:

>100_bases
TCCCACCGCAGCCGATACATCGCAAGATACCCCGGCTGCGGTAAGCGTTACGTATTGATGTAAGTAGCAAAGAAATCAAA
ACATTTCAGGCCACTACAGG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MSGIRHRSPAFRAIIPSSNERRVHMQTFFIDRMATPVGELVLIADEQDRLRAIDWTEHEVRLMKLLNTHYRADRFQLIDK
HNPGGLTEAMQRYFSGELDVIDQLPVMTAGTEFQRSVWQQLRQIPCGEIITYGELAKRIGRPTASRAVGMANGSNPISIV
VPCHRVIGSQGALTGYAGGVQRKQWLLKHEGYLQNDLL

Sequences:

>Translated_198_residues
MSGIRHRSPAFRAIIPSSNERRVHMQTFFIDRMATPVGELVLIADEQDRLRAIDWTEHEVRLMKLLNTHYRADRFQLIDK
HNPGGLTEAMQRYFSGELDVIDQLPVMTAGTEFQRSVWQQLRQIPCGEIITYGELAKRIGRPTASRAVGMANGSNPISIV
VPCHRVIGSQGALTGYAGGVQRKQWLLKHEGYLQNDLL
>Mature_197_residues
SGIRHRSPAFRAIIPSSNERRVHMQTFFIDRMATPVGELVLIADEQDRLRAIDWTEHEVRLMKLLNTHYRADRFQLIDKH
NPGGLTEAMQRYFSGELDVIDQLPVMTAGTEFQRSVWQQLRQIPCGEIITYGELAKRIGRPTASRAVGMANGSNPISIVV
PCHRVIGSQGALTGYAGGVQRKQWLLKHEGYLQNDLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=113, Percent_Identity=40.7079646017699, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787596, Length=169, Percent_Identity=58.5798816568047, Blast_Score=224, Evalue=3e-60,
Organism=Escherichia coli, GI1788542, Length=88, Percent_Identity=51.1363636363636, Blast_Score=101, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=45.679012345679, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=45.679012345679, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=51.8518518518518, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137554, Length=99, Percent_Identity=42.4242424242424, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 22420; Mature: 22289

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIRHRSPAFRAIIPSSNERRVHMQTFFIDRMATPVGELVLIADEQDRLRAIDWTEHEV
CCCCCCCCCCEEEECCCCCCCEEEHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCHHHH
RLMKLLNTHYRADRFQLIDKHNPGGLTEAMQRYFSGELDVIDQLPVMTAGTEFQRSVWQQ
HHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHH
LRQIPCGEIITYGELAKRIGRPTASRAVGMANGSNPISIVVPCHRVIGSQGALTGYAGGV
HHHCCCCCEEHHHHHHHHHCCCCHHHEEECCCCCCCEEEEEEHHHHHCCCCCCCCCCCCH
QRKQWLLKHEGYLQNDLL
HHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
SGIRHRSPAFRAIIPSSNERRVHMQTFFIDRMATPVGELVLIADEQDRLRAIDWTEHEV
CCCCCCCCCEEEECCCCCCCEEEHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCHHHH
RLMKLLNTHYRADRFQLIDKHNPGGLTEAMQRYFSGELDVIDQLPVMTAGTEFQRSVWQQ
HHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHH
LRQIPCGEIITYGELAKRIGRPTASRAVGMANGSNPISIVVPCHRVIGSQGALTGYAGGV
HHHCCCCCEEHHHHHHHHHCCCCHHHEEECCCCCCCEEEEEEHHHHHCCCCCCCCCCCCH
QRKQWLLKHEGYLQNDLL
HHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]