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The map label for this gene is pcaQ [H]

Identifier: 157370767

GI number: 157370767

Start: 2776272

End: 2777204

Strand: Direct

Name: pcaQ [H]

Synonym: Spro_2527

Alternate gene names: 157370767

Gene position: 2776272-2777204 (Clockwise)

Preceding gene: 157370754

Following gene: 157370769

Centisome position: 50.95

GC content: 54.34

Gene sequence:

>933_bases
ATGATCGTGGACAAAATTCTGCGGCAGTTTATCGAAGTAGCGATGTTCAAAAATGTGAGTCATGCAGCAAACAAGCTTTG
CCTGAGCCAGCCAACGCTGACCCACAACATGAAGAAACTGGAAGAAAATCTGGGGGTGCAACTGCTGGAGCGTACCTCAA
CCGGAGTGAAAACCACCGAATACGGTGACCTGCTGCTGGAGCAGGCACAGATGATGCAGCGAATTTACGACAATACGCTG
ATCAAACTGGCATTTATCAAGGCGCGTCAGGAGCAGAGCTTGCGCATGGGCACCGGTCATGCCTGGTGGTATATGTTTGT
GCGCGACAGTTTCAACGCCTACCGCAAGCTGCACCCGTCGGTGAATATCCATATCGATTTGGGTAACCATCTGCGCCTGA
TGGATTTACTGTTGGGCGGCGATATCGATTTGTTTATCGGTCATGAAATTCAGGGGCTGAACCCCAAGGCAGGCATCAGT
TTTCTGCCGCTGTTCGGCACCAGCGACACCATGTTCGTGCGGCGCGGGCATCCATTACTGGGCCAACCGGTAGCGCCGGA
CGCTTTGCTGGACTATCCCTGTGTCGAACTGACGCCGGATGAAAGCCGCTATCATCATATGGTGGAGGATATGCAGCCGA
AAAAGCTGGCGCGCAACCGCCTGCACCTGGCGGAACGGGTGATCTGTTCGACCAACTCGATGATGTCGGCAGTGGATATG
ATCAACGATTCCGACGCGGTGCTGCTGGCCTATCCTGCCTGCATGGCGGGCTATTTTGCCGATTACGGGATCGAGCCATT
GCAACTGACTCAGCCAGGCCAACGCTGTACCGTCGGCATTTATCTGATGCGCGAAAAAGCGGATGCCCCTCACCTGGTGC
AGATGCAAAGGTTAATGCAGCAGCACCTGGAGCCGATCCGCCCTCTGTTGTAA

Upstream 100 bases:

>100_bases
GGAATACCGGCATGGGAGCAAGCTATCGAAGGCGGGTCATTGGCGGAAATCGTTTTTCATCGCTACGATATAGAAACTTT
CTATACCACCAGTGGAAAAT

Downstream 100 bases:

>100_bases
TTGTCATCCAGTCGATAACGCATCATTGGGCGGCCCCGGGGCCTTTCTGGGCATATACTTAACAGAGTGTTCAGGATGGA
GGTAACTGCGATGAAAACAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MIVDKILRQFIEVAMFKNVSHAANKLCLSQPTLTHNMKKLEENLGVQLLERTSTGVKTTEYGDLLLEQAQMMQRIYDNTL
IKLAFIKARQEQSLRMGTGHAWWYMFVRDSFNAYRKLHPSVNIHIDLGNHLRLMDLLLGGDIDLFIGHEIQGLNPKAGIS
FLPLFGTSDTMFVRRGHPLLGQPVAPDALLDYPCVELTPDESRYHHMVEDMQPKKLARNRLHLAERVICSTNSMMSAVDM
INDSDAVLLAYPACMAGYFADYGIEPLQLTQPGQRCTVGIYLMREKADAPHLVQMQRLMQQHLEPIRPLL

Sequences:

>Translated_310_residues
MIVDKILRQFIEVAMFKNVSHAANKLCLSQPTLTHNMKKLEENLGVQLLERTSTGVKTTEYGDLLLEQAQMMQRIYDNTL
IKLAFIKARQEQSLRMGTGHAWWYMFVRDSFNAYRKLHPSVNIHIDLGNHLRLMDLLLGGDIDLFIGHEIQGLNPKAGIS
FLPLFGTSDTMFVRRGHPLLGQPVAPDALLDYPCVELTPDESRYHHMVEDMQPKKLARNRLHLAERVICSTNSMMSAVDM
INDSDAVLLAYPACMAGYFADYGIEPLQLTQPGQRCTVGIYLMREKADAPHLVQMQRLMQQHLEPIRPLL
>Mature_310_residues
MIVDKILRQFIEVAMFKNVSHAANKLCLSQPTLTHNMKKLEENLGVQLLERTSTGVKTTEYGDLLLEQAQMMQRIYDNTL
IKLAFIKARQEQSLRMGTGHAWWYMFVRDSFNAYRKLHPSVNIHIDLGNHLRLMDLLLGGDIDLFIGHEIQGLNPKAGIS
FLPLFGTSDTMFVRRGHPLLGQPVAPDALLDYPCVELTPDESRYHHMVEDMQPKKLARNRLHLAERVICSTNSMMSAVDM
INDSDAVLLAYPACMAGYFADYGIEPLQLTQPGQRCTVGIYLMREKADAPHLVQMQRLMQQHLEPIRPLL

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787601, Length=206, Percent_Identity=28.6407766990291, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI145693105, Length=173, Percent_Identity=27.1676300578035, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35313; Mature: 35313

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVDKILRQFIEVAMFKNVSHAANKLCLSQPTLTHNMKKLEENLGVQLLERTSTGVKTTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCCH
YGDLLLEQAQMMQRIYDNTLIKLAFIKARQEQSLRMGTGHAWWYMFVRDSFNAYRKLHPS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCC
VNIHIDLGNHLRLMDLLLGGDIDLFIGHEIQGLNPKAGISFLPLFGTSDTMFVRRGHPLL
EEEEEECCCCHHHHHHHHCCCEEEEECCEECCCCCCCCCEEEEEECCCCEEEEECCCCCC
GQPVAPDALLDYPCVELTPDESRYHHMVEDMQPKKLARNRLHLAERVICSTNSMMSAVDM
CCCCCCHHHHCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
INDSDAVLLAYPACMAGYFADYGIEPLQLTQPGQRCTVGIYLMREKADAPHLVQMQRLMQ
CCCCCCEEEECHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
QHLEPIRPLL
HHHHHHCCCC
>Mature Secondary Structure
MIVDKILRQFIEVAMFKNVSHAANKLCLSQPTLTHNMKKLEENLGVQLLERTSTGVKTTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCCH
YGDLLLEQAQMMQRIYDNTLIKLAFIKARQEQSLRMGTGHAWWYMFVRDSFNAYRKLHPS
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHCCCC
VNIHIDLGNHLRLMDLLLGGDIDLFIGHEIQGLNPKAGISFLPLFGTSDTMFVRRGHPLL
EEEEEECCCCHHHHHHHHCCCEEEEECCEECCCCCCCCCEEEEEECCCCEEEEECCCCCC
GQPVAPDALLDYPCVELTPDESRYHHMVEDMQPKKLARNRLHLAERVICSTNSMMSAVDM
CCCCCCHHHHCCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
INDSDAVLLAYPACMAGYFADYGIEPLQLTQPGQRCTVGIYLMREKADAPHLVQMQRLMQ
CCCCCCEEEECHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
QHLEPIRPLL
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]