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The map label for this gene is gcvA [H]

Identifier: 157370723

GI number: 157370723

Start: 2724002

End: 2724865

Strand: Direct

Name: gcvA [H]

Synonym: Spro_2483

Alternate gene names: 157370723

Gene position: 2724002-2724865 (Clockwise)

Preceding gene: 157370721

Following gene: 157370724

Centisome position: 49.99

GC content: 59.49

Gene sequence:

>864_bases
ATGCTAAAGCACTGGCCCCCGATGAACGCGTTACGTGGTTTTGAAGCCGCTGCCCGCCTGGGCAGTTTCCACCAGGCCGC
CGATGAGTTGCACCTCACCCAGTCGGCGATCAGTCAGCAAATACGCAGCCTGGAAGCCTATCTGGAACAACCGTTGTTTT
TCCGCAGCGGCCGCAGCGTCAGCCTGACCGACGCCGGGCACGATCTGTACAGCACCGCCCAGGTGATGCTGCAACAACTG
GCAGTCGGCATCCGCCGTCTGGATCAATACCGCAAACCCAACCAACTGATCGTCAATACCACTCCGGCGTTTGCCCGCCA
CTGGCTGGTGCCGAACCTGAAGAGTTTTCATCGGCAGCACCCGGATATCGACCTTTGGCTGTTCACCACCTTCGCCCCAC
CGGATATGGCGACGGAAACCGTCGATCTGACGATCCGTGACGATCTCAGCGCACAAGCCGAATGCAGCCTCCGGGTATTA
CATCAGGACCGACTCTATCCGGCTGGCCATCCTGACGTACTGAAACTGGAGGCTGAGCAACGAACCACTCTGCACGGCGA
ACGTGAGATGGACTGGAGCCATTGGGCCGTTCAGGGCGGCGTCAATGTCGGGCAAAGCAGTCACGGGCTGAATTTTTCAG
ATCCTGGCCTGTTACTGGATGCCGCCTGCGATGGCCTTGGGATCGCGCTGGTCAGCCAATTGCTGGCCCGACAGGCGCTG
GCAAAAGGCGTGTTGCAACCCCTGACGGAACAAAGCGTACGCGGACCCCACTGGGCCTGGCTTGTCCACCGCGACAGCGA
ACATAGTCCTCATACCCGGCAGTTTTGTGAGTGGTTGCTAACCCAGTTGCCGTCCACCGTTTAA

Upstream 100 bases:

>100_bases
GAAAGACGGCGTGGTTTCGATGTAATCATGTTAAAGCTTCCAACGCTGCCATAACAAAGGATACATTTAGCAGACACCTG
ATTAGATAGGCTTATCAATA

Downstream 100 bases:

>100_bases
CGCTCCAGTTCACGGATCCATGCAGCGGATAAAGCATGGCGGATTGAGCGATGCTACCTTCAATCAGGCGCCCGAAAACC
TGCACAGAATGGCGCTAAGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLKHWPPMNALRGFEAAARLGSFHQAADELHLTQSAISQQIRSLEAYLEQPLFFRSGRSVSLTDAGHDLYSTAQVMLQQL
AVGIRRLDQYRKPNQLIVNTTPAFARHWLVPNLKSFHRQHPDIDLWLFTTFAPPDMATETVDLTIRDDLSAQAECSLRVL
HQDRLYPAGHPDVLKLEAEQRTTLHGEREMDWSHWAVQGGVNVGQSSHGLNFSDPGLLLDAACDGLGIALVSQLLARQAL
AKGVLQPLTEQSVRGPHWAWLVHRDSEHSPHTRQFCEWLLTQLPSTV

Sequences:

>Translated_287_residues
MLKHWPPMNALRGFEAAARLGSFHQAADELHLTQSAISQQIRSLEAYLEQPLFFRSGRSVSLTDAGHDLYSTAQVMLQQL
AVGIRRLDQYRKPNQLIVNTTPAFARHWLVPNLKSFHRQHPDIDLWLFTTFAPPDMATETVDLTIRDDLSAQAECSLRVL
HQDRLYPAGHPDVLKLEAEQRTTLHGEREMDWSHWAVQGGVNVGQSSHGLNFSDPGLLLDAACDGLGIALVSQLLARQAL
AKGVLQPLTEQSVRGPHWAWLVHRDSEHSPHTRQFCEWLLTQLPSTV
>Mature_287_residues
MLKHWPPMNALRGFEAAARLGSFHQAADELHLTQSAISQQIRSLEAYLEQPLFFRSGRSVSLTDAGHDLYSTAQVMLQQL
AVGIRRLDQYRKPNQLIVNTTPAFARHWLVPNLKSFHRQHPDIDLWLFTTFAPPDMATETVDLTIRDDLSAQAECSLRVL
HQDRLYPAGHPDVLKLEAEQRTTLHGEREMDWSHWAVQGGVNVGQSSHGLNFSDPGLLLDAACDGLGIALVSQLLARQAL
AKGVLQPLTEQSVRGPHWAWLVHRDSEHSPHTRQFCEWLLTQLPSTV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=31.9587628865979, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=32.2916666666667, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=28.8079470198675, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI157672245, Length=141, Percent_Identity=39.7163120567376, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787128, Length=141, Percent_Identity=35.4609929078014, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=28.4722222222222, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786401, Length=281, Percent_Identity=24.1992882562278, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787589, Length=238, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=123, Percent_Identity=30.8943089430894, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=25.7731958762887, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788297, Length=121, Percent_Identity=33.0578512396694, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32302; Mature: 32302

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHWPPMNALRGFEAAARLGSFHQAADELHLTQSAISQQIRSLEAYLEQPLFFRSGRSV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEE
SLTDAGHDLYSTAQVMLQQLAVGIRRLDQYRKPNQLIVNTTPAFARHWLVPNLKSFHRQH
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHHHHCC
PDIDLWLFTTFAPPDMATETVDLTIRDDLSAQAECSLRVLHQDRLYPAGHPDVLKLEAEQ
CCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECHH
RTTLHGEREMDWSHWAVQGGVNVGQSSHGLNFSDPGLLLDAACDGLGIALVSQLLARQAL
HHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCEEEEHHCCCHHHHHHHHHHHHHHH
AKGVLQPLTEQSVRGPHWAWLVHRDSEHSPHTRQFCEWLLTQLPSTV
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKHWPPMNALRGFEAAARLGSFHQAADELHLTQSAISQQIRSLEAYLEQPLFFRSGRSV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEE
SLTDAGHDLYSTAQVMLQQLAVGIRRLDQYRKPNQLIVNTTPAFARHWLVPNLKSFHRQH
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHHHHCC
PDIDLWLFTTFAPPDMATETVDLTIRDDLSAQAECSLRVLHQDRLYPAGHPDVLKLEAEQ
CCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECHH
RTTLHGEREMDWSHWAVQGGVNVGQSSHGLNFSDPGLLLDAACDGLGIALVSQLLARQAL
HHHCCCCCCCCHHHHEEECCCCCCCCCCCCCCCCCCEEEEHHCCCHHHHHHHHHHHHHHH
AKGVLQPLTEQSVRGPHWAWLVHRDSEHSPHTRQFCEWLLTQLPSTV
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]