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The map label for this gene is ompF [H]

Identifier: 157369701

GI number: 157369701

Start: 1590028

End: 1591125

Strand: Reverse

Name: ompF [H]

Synonym: Spro_1458

Alternate gene names: 157369701

Gene position: 1591125-1590028 (Counterclockwise)

Preceding gene: 157369705

Following gene: 157369700

Centisome position: 29.2

GC content: 50.46

Gene sequence:

>1098_bases
ATGAAACGTAATCTTCTGGCCGTTATTATTCCGGCACTTTTAATTGCGCAGACAGCACAGGCAGCAGAAATCTATAATAA
AGACGGCAACAAACTTGATTTTTACGGCCGCGTAAAAGCACTGCACTATTTCTCTGACGATGCCGGTAATGATGGCGATA
AAACCTATGTGCGTATCGGTTTTAAAGGCGCTACTCAAATTAACGATATGCTGACTGGTTATGGCCAGTGGGAATATCAG
ATTTCAGCTAACCATTCTGAATCCGACAGCGCGAAAGACACCAAGACTCGTCTGGGCTTCGCCGGTCTGAAATATAAAGA
TATCGGTTCATTCGACTACGGCCGCAACTACGGCATCATCTATGACGTCGGCGCCTGGACGGATATGCTGCCTGAGTTCG
GTGATGATGCCTACGTCAAGACCGACAACTTTATGAACGGCCGGACCAACGGCGTAGCCACCTACCGTAACAACGACTTC
TTCGGCCTGGTTGAAGGTCTGAAATTCGCGGTTCAGTACCAGGGCAAAAACGAAAACGACGGCCGCGCCAACAGCAAGGC
GAATGGCGACGGCTGGGGCCTGTCTTCCAGCTATGAAATCATCGACGGCCTGAGCGTCGGTGCTGCCTACGCCTCTTCCA
ACCGTACCGCCGGCCAAAAAGCAGGCACCTTCGGTAAAGGCGATAAAGCGGATATCTGGGCCGGCGGCATGAAATACGAC
AATAACGACGTTTATTTGGCAGCGACCTACTCGCAAAGCCGTAATATCGCGCCTATTTCCGGCACCGCGACCATCAATAA
TACTTCTACCTCCGTTAGCGGCTTTGCCAATAAGGCGGAAGGTCTGGAACTGGTTGCGCAATATCAATTCGACTTCGGTC
TGCGTCCATCGCTGGGTTATGTTCAGCAGAAAGGCAAAGATATTGAAGGCATCGGCGACGCCGACCTGGTGAAATATATC
GACGTTGGCGCTTACTACTATTTCAACAAGAACATGTCGACCTACGTCGATTATAAAATCAACCAACTCGACGGCGATAA
TAAGCTGGGTCTGAAAGACGACAACGTCATCGCCGTCGCCCTGACCTACCAGTTCTGA

Upstream 100 bases:

>100_bases
TACGGTAAAAAACCTGCGCTGCAAACAAAACGGGCGGCACAGTGAAACAGCAGTACAACTGATGATAAGCGTCTTTCAAT
CGAATAAAGCAGGGTAAATC

Downstream 100 bases:

>100_bases
TGTGATGAATAACCACCACTGCAAACCTTAGCTTCACTTGATACCCAGAGTAATTTCGCCGGGCGGCAACGCCCGGTTTT
TTCATTTGGTGCCCTGGCCG

Product: porin

Products: NA

Alternate protein names: Porin ompF [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKRNLLAVIIPALLIAQTAQAAEIYNKDGNKLDFYGRVKALHYFSDDAGNDGDKTYVRIGFKGATQINDMLTGYGQWEYQ
ISANHSESDSAKDTKTRLGFAGLKYKDIGSFDYGRNYGIIYDVGAWTDMLPEFGDDAYVKTDNFMNGRTNGVATYRNNDF
FGLVEGLKFAVQYQGKNENDGRANSKANGDGWGLSSSYEIIDGLSVGAAYASSNRTAGQKAGTFGKGDKADIWAGGMKYD
NNDVYLAATYSQSRNIAPISGTATINNTSTSVSGFANKAEGLELVAQYQFDFGLRPSLGYVQQKGKDIEGIGDADLVKYI
DVGAYYYFNKNMSTYVDYKINQLDGDNKLGLKDDNVIAVALTYQF

Sequences:

>Translated_365_residues
MKRNLLAVIIPALLIAQTAQAAEIYNKDGNKLDFYGRVKALHYFSDDAGNDGDKTYVRIGFKGATQINDMLTGYGQWEYQ
ISANHSESDSAKDTKTRLGFAGLKYKDIGSFDYGRNYGIIYDVGAWTDMLPEFGDDAYVKTDNFMNGRTNGVATYRNNDF
FGLVEGLKFAVQYQGKNENDGRANSKANGDGWGLSSSYEIIDGLSVGAAYASSNRTAGQKAGTFGKGDKADIWAGGMKYD
NNDVYLAATYSQSRNIAPISGTATINNTSTSVSGFANKAEGLELVAQYQFDFGLRPSLGYVQQKGKDIEGIGDADLVKYI
DVGAYYYFNKNMSTYVDYKINQLDGDNKLGLKDDNVIAVALTYQF
>Mature_365_residues
MKRNLLAVIIPALLIAQTAQAAEIYNKDGNKLDFYGRVKALHYFSDDAGNDGDKTYVRIGFKGATQINDMLTGYGQWEYQ
ISANHSESDSAKDTKTRLGFAGLKYKDIGSFDYGRNYGIIYDVGAWTDMLPEFGDDAYVKTDNFMNGRTNGVATYRNNDF
FGLVEGLKFAVQYQGKNENDGRANSKANGDGWGLSSSYEIIDGLSVGAAYASSNRTAGQKAGTFGKGDKADIWAGGMKYD
NNDVYLAATYSQSRNIAPISGTATINNTSTSVSGFANKAEGLELVAQYQFDFGLRPSLGYVQQKGKDIEGIGDADLVKYI
DVGAYYYFNKNMSTYVDYKINQLDGDNKLGLKDDNVIAVALTYQF

Specific function: OmpF is a porin that forms passive diffusion pores which allow small molecular weight hydrophilic materials across the outer membrane. It is also a receptor for the bacteriophage T2 [H]

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1786436, Length=366, Percent_Identity=59.5628415300546, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1787160, Length=371, Percent_Identity=60.1078167115903, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1787641, Length=365, Percent_Identity=57.2602739726027, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1788544, Length=383, Percent_Identity=56.1357702349869, Blast_Score=402, Evalue=1e-113,

Paralogues:

None

Copy number: 794 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 39984; Mature: 39984

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00576 GRAM_NEG_PORIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNLLAVIIPALLIAQTAQAAEIYNKDGNKLDFYGRVKALHYFSDDAGNDGDKTYVRIG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEEE
FKGATQINDMLTGYGQWEYQISANHSESDSAKDTKTRLGFAGLKYKDIGSFDYGRNYGII
ECCCCHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCEEE
YDVGAWTDMLPEFGDDAYVKTDNFMNGRTNGVATYRNNDFFGLVEGLKFAVQYQGKNEND
EECCCHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCEEEHHHCEEEEEEECCCCCCC
GRANSKANGDGWGLSSSYEIIDGLSVGAAYASSNRTAGQKAGTFGKGDKADIWAGGMKYD
CCCCCCCCCCCCCCCCCCCEECCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCEEEC
NNDVYLAATYSQSRNIAPISGTATINNTSTSVSGFANKAEGLELVAQYQFDFGLRPSLGY
CCCEEEEEEECCCCCCCEECCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHH
VQQKGKDIEGIGDADLVKYIDVGAYYYFNKNMSTYVDYKINQLDGDNKLGLKDDNVIAVA
HHHCCCCCCCCCCHHHHHHEECCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCEEEEE
LTYQF
EEECC
>Mature Secondary Structure
MKRNLLAVIIPALLIAQTAQAAEIYNKDGNKLDFYGRVKALHYFSDDAGNDGDKTYVRIG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEEE
FKGATQINDMLTGYGQWEYQISANHSESDSAKDTKTRLGFAGLKYKDIGSFDYGRNYGII
ECCCCHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCEEE
YDVGAWTDMLPEFGDDAYVKTDNFMNGRTNGVATYRNNDFFGLVEGLKFAVQYQGKNEND
EECCCHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCEEEHHHCEEEEEEECCCCCCC
GRANSKANGDGWGLSSSYEIIDGLSVGAAYASSNRTAGQKAGTFGKGDKADIWAGGMKYD
CCCCCCCCCCCCCCCCCCCEECCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCEEEC
NNDVYLAATYSQSRNIAPISGTATINNTSTSVSGFANKAEGLELVAQYQFDFGLRPSLGY
CCCEEEEEEECCCCCCCEECCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHH
VQQKGKDIEGIGDADLVKYIDVGAYYYFNKNMSTYVDYKINQLDGDNKLGLKDDNVIAVA
HHHCCCCCCCCCCHHHHHHEECCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCEEEEE
LTYQF
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9274033 [H]