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The map label for this gene is ubiF [H]

Identifier: 157369464

GI number: 157369464

Start: 1337990

End: 1339231

Strand: Direct

Name: ubiF [H]

Synonym: Spro_1221

Alternate gene names: 157369464

Gene position: 1337990-1339231 (Clockwise)

Preceding gene: 157369451

Following gene: 157369471

Centisome position: 24.56

GC content: 60.47

Gene sequence:

>1242_bases
ATGCCAAACGCCATGCCGGTGGGGATGGCGATAATTAACCAAGAAAAAATAGCCAACAATATGAACACTTCTCAAAAACG
GTATGACGCGGTGGTGGTAGGCGGTGGCATGGTTGGCGCGGCCGCCGCGGTGGGGCTGGCGCAGGCCGGTTGGTCGGTGG
CATTGCTGGAACATCAGGCGCCGCAAGCGTTTGATGCGCAAAGCCCGCCGGACTTGCGTATTTCCGCGATTGGCTGCACC
TCGGTAGGGTTGCTCAAACAGCTCGGTGCCTGGCCGGCGGTGCAAGCGATGCGCACCGCGCCGTACCGCCGTTTGGAAAC
CTGGGAGTGGGCATCGGCACGGGTGGCGTTTGACGCCGCTTCACTCGGCCTGCCGGAGCTGGGCTTTATGGTGGAAAACC
GCATTTTGCAACTGGCGCTGTGGCAGCAACTCGAGCAGTGCGACAATCTGACGCTCTATTGCCCCGCCAGGCTGCGTTCG
CTGCAACGGGGCGATGACCATTGGCAAGTGACACTGGACTCTTCTGAGACGCTTCAGGCGCGTCTGGTGGTCGGGGCTGA
TGGTGCCAACTCGCAGGTGCGCAATTTGGCGGCTATCGGCACCAGCGGCTGGCAGTACCGTCAGTCCTGCATGCTGATCA
CCGTTGAGACCGGTGCGCCGCAGCAGGATGTAACCTGGCAGCAATTTTTCCCTTCGGGCCCACGCGCTTTCCTGCCGCTG
TATGATCAATGGGCCTCGCTGGTGTGGTACGACAGCCCGCAGCGTATTCGTCAGTTGCAGGCGATGCCGCCGGCGCAGCT
TGAGCGCGAAATCGCTGAGGCGTTTCCTGCACGTTTGGGAACGGTGAAAGTGCATGCCGCCGGATCTTTCCCGCTGGCGC
GTCGCCACGCACAGCGCTATGTGTTACCGGGGCTGGCGTTGCTCGGTGACGCGGCGCACACCATTAATCCGTTAGCCGGG
CAGGGCGTGAATCTGGGGTATCGCGATGTGGACGCCTTGCTGGCGGTGTTGAGTGAGGCGCGGGAACAGGGTGAGGACTG
GAGCAGTGAAGCGGTGCTGATGCGCTATCAGCGTCGTCGCCGTACCGATAATTTGCTGATGCAAAGCGGCATGGATCTGT
TTTACACCGCCTTTAGCAATAATTTGGCACCGTTAAGCGTGGCACGCAATCTTGCGTTGATGGCGGCACAACGGGCGGGC
AAGCTAAAGGAAAATGCGTTGAAGTACGCGCTGGGTTTATAA

Upstream 100 bases:

>100_bases
CGCGACATGCAGGGCGCTATTGTAAACATTTGACGCCGTTGTGACCAGCGCGGGGAAAACGGCATGCGACCTGAAGCCAA
ATTAAAAATCCGTTACACTG

Downstream 100 bases:

>100_bases
CCTGAACGGGTTCAGCTTGCTGGGCCCGGTCATTTGCCGAATGACAGACAGCAAAAAGCCCGCCGAAGCGGGCTTTTCTA
AATGTGGCTGGGGTGCCAGG

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MPNAMPVGMAIINQEKIANNMNTSQKRYDAVVVGGGMVGAAAAVGLAQAGWSVALLEHQAPQAFDAQSPPDLRISAIGCT
SVGLLKQLGAWPAVQAMRTAPYRRLETWEWASARVAFDAASLGLPELGFMVENRILQLALWQQLEQCDNLTLYCPARLRS
LQRGDDHWQVTLDSSETLQARLVVGADGANSQVRNLAAIGTSGWQYRQSCMLITVETGAPQQDVTWQQFFPSGPRAFLPL
YDQWASLVWYDSPQRIRQLQAMPPAQLEREIAEAFPARLGTVKVHAAGSFPLARRHAQRYVLPGLALLGDAAHTINPLAG
QGVNLGYRDVDALLAVLSEAREQGEDWSSEAVLMRYQRRRRTDNLLMQSGMDLFYTAFSNNLAPLSVARNLALMAAQRAG
KLKENALKYALGL

Sequences:

>Translated_413_residues
MPNAMPVGMAIINQEKIANNMNTSQKRYDAVVVGGGMVGAAAAVGLAQAGWSVALLEHQAPQAFDAQSPPDLRISAIGCT
SVGLLKQLGAWPAVQAMRTAPYRRLETWEWASARVAFDAASLGLPELGFMVENRILQLALWQQLEQCDNLTLYCPARLRS
LQRGDDHWQVTLDSSETLQARLVVGADGANSQVRNLAAIGTSGWQYRQSCMLITVETGAPQQDVTWQQFFPSGPRAFLPL
YDQWASLVWYDSPQRIRQLQAMPPAQLEREIAEAFPARLGTVKVHAAGSFPLARRHAQRYVLPGLALLGDAAHTINPLAG
QGVNLGYRDVDALLAVLSEAREQGEDWSSEAVLMRYQRRRRTDNLLMQSGMDLFYTAFSNNLAPLSVARNLALMAAQRAG
KLKENALKYALGL
>Mature_412_residues
PNAMPVGMAIINQEKIANNMNTSQKRYDAVVVGGGMVGAAAAVGLAQAGWSVALLEHQAPQAFDAQSPPDLRISAIGCTS
VGLLKQLGAWPAVQAMRTAPYRRLETWEWASARVAFDAASLGLPELGFMVENRILQLALWQQLEQCDNLTLYCPARLRSL
QRGDDHWQVTLDSSETLQARLVVGADGANSQVRNLAAIGTSGWQYRQSCMLITVETGAPQQDVTWQQFFPSGPRAFLPLY
DQWASLVWYDSPQRIRQLQAMPPAQLEREIAEAFPARLGTVKVHAAGSFPLARRHAQRYVLPGLALLGDAAHTINPLAGQ
GVNLGYRDVDALLAVLSEAREQGEDWSSEAVLMRYQRRRRTDNLLMQSGMDLFYTAFSNNLAPLSVARNLALMAAQRAGK
LKENALKYALGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=383, Percent_Identity=32.8981723237598, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI32967307, Length=348, Percent_Identity=32.4712643678161, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1786883, Length=385, Percent_Identity=64.9350649350649, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1789273, Length=388, Percent_Identity=39.6907216494845, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789274, Length=372, Percent_Identity=33.6021505376344, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25146631, Length=431, Percent_Identity=28.538283062645, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321694, Length=459, Percent_Identity=25.2723311546841, Blast_Score=135, Evalue=9e-33,
Organism=Drosophila melanogaster, GI19920760, Length=436, Percent_Identity=29.5871559633028, Blast_Score=190, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 45282; Mature: 45151

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNAMPVGMAIINQEKIANNMNTSQKRYDAVVVGGGMVGAAAAVGLAQAGWSVALLEHQA
CCCCCCCCHHHHHHHHHHCCCCCCHHHCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
PQAFDAQSPPDLRISAIGCTSVGLLKQLGAWPAVQAMRTAPYRRLETWEWASARVAFDAA
CCCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCEEEEEHH
SLGLPELGFMVENRILQLALWQQLEQCDNLTLYCPARLRSLQRGDDHWQVTLDSSETLQA
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCCEEEEECCCCCEEE
RLVVGADGANSQVRNLAAIGTSGWQYRQSCMLITVETGAPQQDVTWQQFFPSGPRAFLPL
EEEEECCCCCHHHHHHHHCCCCCHHCCCCEEEEEEECCCCCCCCCHHHHCCCCCCEECCH
YDQWASLVWYDSPQRIRQLQAMPPAQLEREIAEAFPARLGTVKVHAAGSFPLARRHAQRY
HHHHHHEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
VLPGLALLGDAAHTINPLAGQGVNLGYRDVDALLAVLSEAREQGEDWSSEAVLMRYQRRR
HCCCHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RTDNLLMQSGMDLFYTAFSNNLAPLSVARNLALMAAQRAGKLKENALKYALGL
HHHHHHHHCCCHHEEHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
PNAMPVGMAIINQEKIANNMNTSQKRYDAVVVGGGMVGAAAAVGLAQAGWSVALLEHQA
CCCCCCCHHHHHHHHHHCCCCCCHHHCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
PQAFDAQSPPDLRISAIGCTSVGLLKQLGAWPAVQAMRTAPYRRLETWEWASARVAFDAA
CCCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCEEEEEHH
SLGLPELGFMVENRILQLALWQQLEQCDNLTLYCPARLRSLQRGDDHWQVTLDSSETLQA
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCCEEEEECCCCCEEE
RLVVGADGANSQVRNLAAIGTSGWQYRQSCMLITVETGAPQQDVTWQQFFPSGPRAFLPL
EEEEECCCCCHHHHHHHHCCCCCHHCCCCEEEEEEECCCCCCCCCHHHHCCCCCCEECCH
YDQWASLVWYDSPQRIRQLQAMPPAQLEREIAEAFPARLGTVKVHAAGSFPLARRHAQRY
HHHHHHEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
VLPGLALLGDAAHTINPLAGQGVNLGYRDVDALLAVLSEAREQGEDWSSEAVLMRYQRRR
HCCCHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RTDNLLMQSGMDLFYTAFSNNLAPLSVARNLALMAAQRAGKLKENALKYALGL
HHHHHHHHCCCHHEEHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]