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The map label for this gene is cysS

Identifier: 157369409

GI number: 157369409

Start: 1283158

End: 1284543

Strand: Direct

Name: cysS

Synonym: Spro_1166

Alternate gene names: 157369409

Gene position: 1283158-1284543 (Clockwise)

Preceding gene: 157369404

Following gene: 157369412

Centisome position: 23.55

GC content: 56.28

Gene sequence:

>1386_bases
ATGCTAAAGATTTTTAATACCCTGAGTCGTCAAAAAGAGGAATTTAAGCCCATTCATGCCGGTAAAGTTGGCATGTACGT
GTGCGGGGTAACCATCTATGACCTGTGTCATATCGGCCACGGGCGTACCTTTGTTGCTTTCGACGTCGTCGCGCGTTATT
TGCGTTACCTCGGTTATTCGCTGAACTACGTGCGTAACGTTACCGACGTTGACGATAAAATTATCCGCCGTGCGACAGAG
AATGGCGAAAGCTGCGATCAACTGACCACGCGCATGCTGGCAGAGATGCACGCCGACTTCGATTCTCTGCTGATTGAACG
CCCGGACATCGAACCGCGCGCCACTCAGCATATCGCCGAGATCGTTGAGATCACCCAGCGCCTGATCGATCGCGGTCATG
CCTACGTGGCCAGCAACGGCGACGTGATGTTCTCTATCGACAGCGATCCGCAATACGGCTTGTTGTCACGCCAGGATCTG
GATCAGCTGCAGGCCGGTGCGCGGGTAGAAATCGATGATGTAAAACGCAACCCGATGGATTTCGTGCTGTGGAAGATGTC
CAAGCCGGGCGAACCGAGCTGGGAATCGCCATGGGGCGCAGGCCGTCCGGGCTGGCACATTGAATGTTCTGCGATGAACT
GCAAGCAGTTGGGCACCCATTTTGACATCCACGGCGGCGGTTCCGATCTGATGTTCCCGCATCACGAGAACGAAATTGCC
CAGTCCAGCTGTGCTCACGACGGCCCGTACGTCAACTACTGGATGCACTCCGGTATGGTGATGATCGACAAAGAAAAAAT
GTCGAAATCGCTGGATAACTTCTTCACCATCCGCGACGTGCTGGGTTACTACGACGCCGAAACCGTGCGTTACTTCCTGA
TGTCTGGCCACTATCGCAGCCAACTGAACTACAGCGAAGAGAACCTGAAACAGGCGCGTACCGCACTGGAGCGTTTGTAC
ACCGCGCTGCGCGGTACCGACGTTAACGCTCAACCGGCCGGTGGCGAAGTGTTTGAAGCTCGCTTCCGCGAAGCCATGGA
TGATGACTTCAATACCCCGGAAGCTTACTCGGCGCTGTTTGACTTGGCACGCGAGGTTAACCGCCTGAAAGCGGAAGATC
TGACTGCAGCCAACGGCCTGGCGGCGGAACTGCGCAAGCTGGCGAAGGTGCTGGGCTTGTTGCAGCAAGAACCGGAACTG
TTCCTGCAGGGCGGCGCACAGGCGGACGACGGTGAAGTGGCGGAGATCGAGGCACTGATCAAACAGCGTAATGACGCGCG
CAAAGCCAAAGATTGGGCGCTGGCGGATGCCGCGCGCGACCGTTTGAACGAGATGAACATTGTGCTGGAAGATGGTCCGC
AGGGAACCACCTGGCGCCGTAAGTAA

Upstream 100 bases:

>100_bases
GCCTTAACCTTGCATTAGATCAGGCTTCGGGTTTCAATACGCTGCTGGGTGAATCGCCTAATTACTATTTTTGTTATCAC
GCACACCACGGAATGTCCCG

Downstream 100 bases:

>100_bases
TTCGCTCTAGGCTGAAAAGCAAAAAAGGCGCTGAGGGTTAACTCAGCGCCTTTTTCTATGGAGCACGGCTTATGGTTTAA
CTACAACCTTGCCGCCGTTA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLKIFNTLSRQKEEFKPIHAGKVGMYVCGVTIYDLCHIGHGRTFVAFDVVARYLRYLGYSLNYVRNVTDVDDKIIRRATE
NGESCDQLTTRMLAEMHADFDSLLIERPDIEPRATQHIAEIVEITQRLIDRGHAYVASNGDVMFSIDSDPQYGLLSRQDL
DQLQAGARVEIDDVKRNPMDFVLWKMSKPGEPSWESPWGAGRPGWHIECSAMNCKQLGTHFDIHGGGSDLMFPHHENEIA
QSSCAHDGPYVNYWMHSGMVMIDKEKMSKSLDNFFTIRDVLGYYDAETVRYFLMSGHYRSQLNYSEENLKQARTALERLY
TALRGTDVNAQPAGGEVFEARFREAMDDDFNTPEAYSALFDLAREVNRLKAEDLTAANGLAAELRKLAKVLGLLQQEPEL
FLQGGAQADDGEVAEIEALIKQRNDARKAKDWALADAARDRLNEMNIVLEDGPQGTTWRRK

Sequences:

>Translated_461_residues
MLKIFNTLSRQKEEFKPIHAGKVGMYVCGVTIYDLCHIGHGRTFVAFDVVARYLRYLGYSLNYVRNVTDVDDKIIRRATE
NGESCDQLTTRMLAEMHADFDSLLIERPDIEPRATQHIAEIVEITQRLIDRGHAYVASNGDVMFSIDSDPQYGLLSRQDL
DQLQAGARVEIDDVKRNPMDFVLWKMSKPGEPSWESPWGAGRPGWHIECSAMNCKQLGTHFDIHGGGSDLMFPHHENEIA
QSSCAHDGPYVNYWMHSGMVMIDKEKMSKSLDNFFTIRDVLGYYDAETVRYFLMSGHYRSQLNYSEENLKQARTALERLY
TALRGTDVNAQPAGGEVFEARFREAMDDDFNTPEAYSALFDLAREVNRLKAEDLTAANGLAAELRKLAKVLGLLQQEPEL
FLQGGAQADDGEVAEIEALIKQRNDARKAKDWALADAARDRLNEMNIVLEDGPQGTTWRRK
>Mature_461_residues
MLKIFNTLSRQKEEFKPIHAGKVGMYVCGVTIYDLCHIGHGRTFVAFDVVARYLRYLGYSLNYVRNVTDVDDKIIRRATE
NGESCDQLTTRMLAEMHADFDSLLIERPDIEPRATQHIAEIVEITQRLIDRGHAYVASNGDVMFSIDSDPQYGLLSRQDL
DQLQAGARVEIDDVKRNPMDFVLWKMSKPGEPSWESPWGAGRPGWHIECSAMNCKQLGTHFDIHGGGSDLMFPHHENEIA
QSSCAHDGPYVNYWMHSGMVMIDKEKMSKSLDNFFTIRDVLGYYDAETVRYFLMSGHYRSQLNYSEENLKQARTALERLY
TALRGTDVNAQPAGGEVFEARFREAMDDDFNTPEAYSALFDLAREVNRLKAEDLTAANGLAAELRKLAKVLGLLQQEPEL
FLQGGAQADDGEVAEIEALIKQRNDARKAKDWALADAARDRLNEMNIVLEDGPQGTTWRRK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=479, Percent_Identity=34.446764091858, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI10835051, Length=233, Percent_Identity=42.9184549356223, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI21269877, Length=233, Percent_Identity=42.9184549356223, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI62240992, Length=233, Percent_Identity=42.9184549356223, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI303304987, Length=232, Percent_Identity=43.1034482758621, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1786737, Length=461, Percent_Identity=83.5140997830803, Blast_Score=796, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17509797, Length=246, Percent_Identity=39.4308943089431, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17509795, Length=246, Percent_Identity=39.4308943089431, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6324082, Length=397, Percent_Identity=31.9899244332494, Blast_Score=202, Evalue=7e-53,
Organism=Drosophila melanogaster, GI19922218, Length=502, Percent_Identity=33.2669322709163, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19922364, Length=232, Percent_Identity=42.2413793103448, Blast_Score=178, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_SERP5 (A8GAY0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477398.1
- ProteinModelPortal:   A8GAY0
- SMR:   A8GAY0
- STRING:   A8GAY0
- GeneID:   5602723
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1166
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   SPRO399741:SPRO_1166-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52272; Mature: 52272

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIFNTLSRQKEEFKPIHAGKVGMYVCGVTIYDLCHIGHGRTFVAFDVVARYLRYLGYS
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCH
LNYVRNVTDVDDKIIRRATENGESCDQLTTRMLAEMHADFDSLLIERPDIEPRATQHIAE
HHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHH
IVEITQRLIDRGHAYVASNGDVMFSIDSDPQYGLLSRQDLDQLQAGARVEIDDVKRNPMD
HHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEHHCCCCCCC
FVLWKMSKPGEPSWESPWGAGRPGWHIECSAMNCKQLGTHFDIHGGGSDLMFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCEEEECCCCCCCCCCCCCCHHH
QSSCAHDGPYVNYWMHSGMVMIDKEKMSKSLDNFFTIRDVLGYYDAETVRYFLMSGHYRS
HHHCCCCCCEEEEEHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QLNYSEENLKQARTALERLYTALRGTDVNAQPAGGEVFEARFREAMDDDFNTPEAYSALF
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
DLAREVNRLKAEDLTAANGLAAELRKLAKVLGLLQQEPELFLQGGAQADDGEVAEIEALI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
KQRNDARKAKDWALADAARDRLNEMNIVLEDGPQGTTWRRK
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLKIFNTLSRQKEEFKPIHAGKVGMYVCGVTIYDLCHIGHGRTFVAFDVVARYLRYLGYS
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCH
LNYVRNVTDVDDKIIRRATENGESCDQLTTRMLAEMHADFDSLLIERPDIEPRATQHIAE
HHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHH
IVEITQRLIDRGHAYVASNGDVMFSIDSDPQYGLLSRQDLDQLQAGARVEIDDVKRNPMD
HHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEHHCCCCCCC
FVLWKMSKPGEPSWESPWGAGRPGWHIECSAMNCKQLGTHFDIHGGGSDLMFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHCCCEEEECCCCCCCCCCCCCCHHH
QSSCAHDGPYVNYWMHSGMVMIDKEKMSKSLDNFFTIRDVLGYYDAETVRYFLMSGHYRS
HHHCCCCCCEEEEEHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
QLNYSEENLKQARTALERLYTALRGTDVNAQPAGGEVFEARFREAMDDDFNTPEAYSALF
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
DLAREVNRLKAEDLTAANGLAAELRKLAKVLGLLQQEPELFLQGGAQADDGEVAEIEALI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
KQRNDARKAKDWALADAARDRLNEMNIVLEDGPQGTTWRRK
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA