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The map label for this gene is phsB [H]

Identifier: 157369310

GI number: 157369310

Start: 1174824

End: 1175558

Strand: Direct

Name: phsB [H]

Synonym: Spro_1067

Alternate gene names: 157369310

Gene position: 1174824-1175558 (Clockwise)

Preceding gene: 157369307

Following gene: 157369311

Centisome position: 21.56

GC content: 59.59

Gene sequence:

>735_bases
ATGGACAGTGGGAAACGGCAGTTTTTACAACGACTCGGTGCATTGACTGCCGGCGCAGCTCTGGTGCCGCTGGCGCAGTC
AGGCTGGCAGTTGCAGCCGGCTCGGCGGGAAGGTGACAGCAAAAAGCGCATGGCGATGCTGATCGACCTGCGCCGCTGCA
TTGGCTGTCAGGCCTGTACCGTTAGCTGTGCGGTGGAAAATCGGCTACCGCAGGGGCAATTTCGCACCAGCGTACTGCAA
TATCAGGTCAGCCTGACGGGGCAGAACTCGACCACCAACGTGCTGCTGCCACGGTTGTGCAACCACTGTGACAATCCGCC
TTGCGTGCCGGTCTGCCCGGTGCAGGCGACTTTCCAGCGTCAGGACGGCATTGTGGTTGTCGACAATACCCGCTGCGTCG
GCTGTGCCTATTGCGTGCAGGCCTGCCCTTATGAGGCTAGGTTTATCAACCATGAAACCCAGACCGCCGACAAGTGTACT
TTCTGCGTGCATCGGCTGGATGCCGGGCTGTTGCCCGCCTGCGTCGAGTCCTGTGTCGGTGGTGCGCGGATGATTGGCGA
TCTTAACGATCGGCAAGGTGCATTAAGGCGGGCTCTGGATGAGCATCAGGCACAGCTCAAGGTACTGAAACCCGAACAGG
GGACACATCCTCAGGTGTTCTATCTGGGGCTTGATGAGGCATTTGTCAGTCACGTCCGGGGACAACCGGCACTGTGGCAG
GAGGTGCATCCATGA

Upstream 100 bases:

>100_bases
CCGCCGTGGGGAATGTGGTAAACCACAATATTGGCGGTCGGTCGCTGTTTTTACACTCCTTTGTATCAGTTCCCCGGCGG
ATACTTATTGGGAGAAATCA

Downstream 100 bases:

>100_bases
TCCGCGAAATTATGGCTCGCCCGCAGGAGATCGCCTGGTTGCCCTGGGCGGTGCAGTATTTTTTCTTTATTGGCCTGGCA
TGCGGCAGCATCATGATTGC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MDSGKRQFLQRLGALTAGAALVPLAQSGWQLQPARREGDSKKRMAMLIDLRRCIGCQACTVSCAVENRLPQGQFRTSVLQ
YQVSLTGQNSTTNVLLPRLCNHCDNPPCVPVCPVQATFQRQDGIVVVDNTRCVGCAYCVQACPYEARFINHETQTADKCT
FCVHRLDAGLLPACVESCVGGARMIGDLNDRQGALRRALDEHQAQLKVLKPEQGTHPQVFYLGLDEAFVSHVRGQPALWQ
EVHP

Sequences:

>Translated_244_residues
MDSGKRQFLQRLGALTAGAALVPLAQSGWQLQPARREGDSKKRMAMLIDLRRCIGCQACTVSCAVENRLPQGQFRTSVLQ
YQVSLTGQNSTTNVLLPRLCNHCDNPPCVPVCPVQATFQRQDGIVVVDNTRCVGCAYCVQACPYEARFINHETQTADKCT
FCVHRLDAGLLPACVESCVGGARMIGDLNDRQGALRRALDEHQAQLKVLKPEQGTHPQVFYLGLDEAFVSHVRGQPALWQ
EVHP
>Mature_244_residues
MDSGKRQFLQRLGALTAGAALVPLAQSGWQLQPARREGDSKKRMAMLIDLRRCIGCQACTVSCAVENRLPQGQFRTSVLQ
YQVSLTGQNSTTNVLLPRLCNHCDNPPCVPVCPVQATFQRQDGIVVVDNTRCVGCAYCVQACPYEARFINHETQTADKCT
FCVHRLDAGLLPACVESCVGGARMIGDLNDRQGALRRALDEHQAQLKVLKPEQGTHPQVFYLGLDEAFVSHVRGQPALWQ
EVHP

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=236, Percent_Identity=36.0169491525424, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI226510944, Length=180, Percent_Identity=35.5555555555556, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787749, Length=230, Percent_Identity=30.4347826086957, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790326, Length=226, Percent_Identity=30.5309734513274, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787478, Length=127, Percent_Identity=38.5826771653543, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787872, Length=189, Percent_Identity=35.4497354497354, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787740, Length=136, Percent_Identity=36.0294117647059, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787122, Length=189, Percent_Identity=33.8624338624339, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789370, Length=222, Percent_Identity=32.4324324324324, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082180, Length=198, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI2367245, Length=151, Percent_Identity=33.7748344370861, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787963, Length=189, Percent_Identity=28.042328042328, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789067, Length=162, Percent_Identity=31.4814814814815, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082179, Length=137, Percent_Identity=32.8467153284672, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788811, Length=177, Percent_Identity=30.5084745762712, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 26871; Mature: 26871

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
6.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGKRQFLQRLGALTAGAALVPLAQSGWQLQPARREGDSKKRMAMLIDLRRCIGCQACT
CCCHHHHHHHHHHHHHCCHHHHHHHCCCCEECCHHHCCCHHHHHHHHHHHHHHHCCHHHE
VSCAVENRLPQGQFRTSVLQYQVSLTGQNSTTNVLLPRLCNHCDNPPCVPVCPVQATFQR
EEEHHHCCCCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHCC
QDGIVVVDNTRCVGCAYCVQACPYEARFINHETQTADKCTFCVHRLDAGLLPACVESCVG
CCCEEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCC
GARMIGDLNDRQGALRRALDEHQAQLKVLKPEQGTHPQVFYLGLDEAFVSHVRGQPALWQ
CHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEECHHHHHHHHCCCCCHHH
EVHP
CCCC
>Mature Secondary Structure
MDSGKRQFLQRLGALTAGAALVPLAQSGWQLQPARREGDSKKRMAMLIDLRRCIGCQACT
CCCHHHHHHHHHHHHHCCHHHHHHHCCCCEECCHHHCCCHHHHHHHHHHHHHHHCCHHHE
VSCAVENRLPQGQFRTSVLQYQVSLTGQNSTTNVLLPRLCNHCDNPPCVPVCPVQATFQR
EEEHHHCCCCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHCC
QDGIVVVDNTRCVGCAYCVQACPYEARFINHETQTADKCTFCVHRLDAGLLPACVESCVG
CCCEEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCC
GARMIGDLNDRQGALRRALDEHQAQLKVLKPEQGTHPQVFYLGLDEAFVSHVRGQPALWQ
CHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEECHHHHHHHHCCCCCHHH
EVHP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]