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The map label for this gene is brnQ [H]

Identifier: 157369288

GI number: 157369288

Start: 1149404

End: 1150723

Strand: Direct

Name: brnQ [H]

Synonym: Spro_1045

Alternate gene names: 157369288

Gene position: 1149404-1150723 (Clockwise)

Preceding gene: 157369287

Following gene: 157369289

Centisome position: 21.09

GC content: 58.79

Gene sequence:

>1320_bases
ATGAGTCATCGTTTAACGTCAAAAGATATTATTGCACTGGGTTTTATGACCTTTGCCTTATTCGTCGGCGCCGGGAATAT
CATTTTCCCGCCGATGGTCGGTTTACAGTCCGGTGAACACGTCTGGACTGCAGCTATCGGCTTCCTGATCACTGCCGTCG
GTCTACCGGTGATGACGGTGATCGCGTTGGCTCGTGTCGGTGGCGGCATTGACGCCCTGAGCACGCCAATTGGTCGTCGC
GCCGGCCTGCTGCTGGCGACCGTCTGCTATCTGGCCGTCGGCCCACTATTCGCCACCCCGCGCACCGCGACCGTCTCCTT
CGAAGTGGGCATTGCACCGCTGACCGGTGACGGTGCCATGCCGCTGTTTATTTACAGCCTGGTGTATTTCGCACTGGTGA
TCGGCATCTCTCTGTATCCGGGCCGCTTGCTCGATACCGTCGGCCACGTACTGGCGCCGCTGAAAATTGTTGCGCTGGGC
GCATTGGGTATTGCCGCGCTGGCCTGGCCTGCGGGCACGCCAATTCCGGCCACCGAGGTCTATCAGAACGTTCCATTCTC
TACCGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCGCTGGTCTTCGGCATCGTCATCGTTAACGCCGCGC
GTTCCCGTGGCGTGAAGGATGCCAAATTGCTGACGCGTTACACCATTCTGGCGGGTCTGATCGCCGGTGTCGGCCTGACG
CTGGTTTACCTGAGCCTGTTCAAGCTGGGTTCCGGCAGCGGTGTGCTGGTGCCGGACGCCACCAACGGCGCGGTGATCTT
GCATGCTTACGTACAGAACACTTTCGGTGGATTGGGCAGCTTCTTCCTGGCCGCGCTGATCTTCATTGCCTGTATGGTGA
CTGCGGTTGGCCTGACCTGCGCCTGTGCCGAGTTCTTTGCCCAATACCTGCCGTTCTCTTACAAGACGCTGGTGTTCATT
TTGGGTCTGTTCTCGATGCTGGTTTCCAACCTGGGCCTGAGCCATCTGATCCAGATCTCTATCCCGGTGCTGACCGCGAT
TTATCCGCCGTGTATCGTGCTGGTGGTGCTGAGTTTCACTCAGCGCTGGTGGAACAGCGCGCCGCGTATCATCGCGCCGG
TGATGTTAGTCAGCCTGTTGTTTGGTATCCTTGACGCGGTGAAAGCGTCCACCTTCCAGCACTTCCTGCCGGAGTGGACC
AATCACCTGCCATTGGCGGATCAAGGGCTGGCATGGTTGCCGCCGTCGCTGCTGATGCTGGTGCTGGTCGCAATTTATGA
CCGGGTTTGTTCGCGCTCCGAAGTGACCGCGCACTCTTAA

Upstream 100 bases:

>100_bases
TTTGGTCTCGCGATGAAATGGCAAGCCGGCAACCGGGTGGGGCATAACGCCACCACGTATCGGACACTTTTTTTCATTTG
AACAGGCAACACGACATCGT

Downstream 100 bases:

>100_bases
TTCCCCGGCTAAATTTTGGGCCACGGACTTGTCCGTGGCTTTTTTACGTTTAAAAAACACGGGTTATCTTCATGCAACAA
CAGTCACCCGCCGCTCCTGC

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSHRLTSKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHVWTAAIGFLITAVGLPVMTVIALARVGGGIDALSTPIGRR
AGLLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDGAMPLFIYSLVYFALVIGISLYPGRLLDTVGHVLAPLKIVALG
ALGIAALAWPAGTPIPATEVYQNVPFSTGFVNGYLTMDTLGALVFGIVIVNAARSRGVKDAKLLTRYTILAGLIAGVGLT
LVYLSLFKLGSGSGVLVPDATNGAVILHAYVQNTFGGLGSFFLAALIFIACMVTAVGLTCACAEFFAQYLPFSYKTLVFI
LGLFSMLVSNLGLSHLIQISIPVLTAIYPPCIVLVVLSFTQRWWNSAPRIIAPVMLVSLLFGILDAVKASTFQHFLPEWT
NHLPLADQGLAWLPPSLLMLVLVAIYDRVCSRSEVTAHS

Sequences:

>Translated_439_residues
MSHRLTSKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHVWTAAIGFLITAVGLPVMTVIALARVGGGIDALSTPIGRR
AGLLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDGAMPLFIYSLVYFALVIGISLYPGRLLDTVGHVLAPLKIVALG
ALGIAALAWPAGTPIPATEVYQNVPFSTGFVNGYLTMDTLGALVFGIVIVNAARSRGVKDAKLLTRYTILAGLIAGVGLT
LVYLSLFKLGSGSGVLVPDATNGAVILHAYVQNTFGGLGSFFLAALIFIACMVTAVGLTCACAEFFAQYLPFSYKTLVFI
LGLFSMLVSNLGLSHLIQISIPVLTAIYPPCIVLVVLSFTQRWWNSAPRIIAPVMLVSLLFGILDAVKASTFQHFLPEWT
NHLPLADQGLAWLPPSLLMLVLVAIYDRVCSRSEVTAHS
>Mature_438_residues
SHRLTSKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHVWTAAIGFLITAVGLPVMTVIALARVGGGIDALSTPIGRRA
GLLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDGAMPLFIYSLVYFALVIGISLYPGRLLDTVGHVLAPLKIVALGA
LGIAALAWPAGTPIPATEVYQNVPFSTGFVNGYLTMDTLGALVFGIVIVNAARSRGVKDAKLLTRYTILAGLIAGVGLTL
VYLSLFKLGSGSGVLVPDATNGAVILHAYVQNTFGGLGSFFLAALIFIACMVTAVGLTCACAEFFAQYLPFSYKTLVFIL
GLFSMLVSNLGLSHLIQISIPVLTAIYPPCIVLVVLSFTQRWWNSAPRIIAPVMLVSLLFGILDAVKASTFQHFLPEWTN
HLPLADQGLAWLPPSLLMLVLVAIYDRVCSRSEVTAHS

Specific function: Component of the LIV-II transport system for branched- chain amino acids. This LIV-II transport system may be H(+)- coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=439, Percent_Identity=76.3097949886105, Blast_Score=672, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46370; Mature: 46239

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRLTSKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHVWTAAIGFLITAVGLPVMTV
CCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IALARVGGGIDALSTPIGRRAGLLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDGAM
HHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCH
PLFIYSLVYFALVIGISLYPGRLLDTVGHVLAPLKIVALGALGIAALAWPAGTPIPATEV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
YQNVPFSTGFVNGYLTMDTLGALVFGIVIVNAARSRGVKDAKLLTRYTILAGLIAGVGLT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LVYLSLFKLGSGSGVLVPDATNGAVILHAYVQNTFGGLGSFFLAALIFIACMVTAVGLTC
HHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ACAEFFAQYLPFSYKTLVFILGLFSMLVSNLGLSHLIQISIPVLTAIYPPCIVLVVLSFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRWWNSAPRIIAPVMLVSLLFGILDAVKASTFQHFLPEWTNHLPLADQGLAWLPPSLLML
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
VLVAIYDRVCSRSEVTAHS
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SHRLTSKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHVWTAAIGFLITAVGLPVMTV
CCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IALARVGGGIDALSTPIGRRAGLLLATVCYLAVGPLFATPRTATVSFEVGIAPLTGDGAM
HHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCH
PLFIYSLVYFALVIGISLYPGRLLDTVGHVLAPLKIVALGALGIAALAWPAGTPIPATEV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
YQNVPFSTGFVNGYLTMDTLGALVFGIVIVNAARSRGVKDAKLLTRYTILAGLIAGVGLT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LVYLSLFKLGSGSGVLVPDATNGAVILHAYVQNTFGGLGSFFLAALIFIACMVTAVGLTC
HHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ACAEFFAQYLPFSYKTLVFILGLFSMLVSNLGLSHLIQISIPVLTAIYPPCIVLVVLSFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRWWNSAPRIIAPVMLVSLLFGILDAVKASTFQHFLPEWTNHLPLADQGLAWLPPSLLML
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
VLVAIYDRVCSRSEVTAHS
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]