Click here to switch to the map view.

The map label for this gene is leuA

Identifier: 157368990

GI number: 157368990

Start: 828859

End: 830433

Strand: Reverse

Name: leuA

Synonym: Spro_0745

Alternate gene names: 157368990

Gene position: 830433-828859 (Counterclockwise)

Preceding gene: 157368996

Following gene: 157368989

Centisome position: 15.24

GC content: 54.6

Gene sequence:

>1575_bases
ATGAGCCAACAAGTCATTATTTTCGATACAACGCTGCGTGATGGTGAACAAGCGCTGCAAGCCAGCCTGAGCGTGAAAGA
GAAGATTCAGATCGCGATGGCGCTGGAAAGAATGGGTGTCGACGTCATGGAAGTCGGCTTCCCGGTCTCCTCGCCGGGCG
ATTTTGAATCTGTGCAAACCATCGCGCGCCAGATTAAAAACAGCCGCGTATGTGGCCTGGCCCGTTGCGTGGATAAAGAT
ATCGACGTCGCAGCCGAAGCGTTGCGCGTAGCCGAAGCCTTCCGTATTCACGTGTTCCTGGCCACCTCAACCCTGCATAT
TGAATCAAAACTGAAGCGCTCGTTCGACGAAGTGCTGGAGATGGCGATCCGCTCGGTGAAACGCGCCCGTAATTACACCG
ACGACGTAGAATTCTCCTGTGAAGACGCAGGCCGTACGCCAATCGATAACCTGTGTCGCGTGGTAGAAGCCGCGATCAAC
GCCGGTGCCACCACCATTAACATTCCGGATACCGTGGGCTACACCACGCCGAACCAGTTTGGTGGCATTATCACTACCCT
GTATGACCGCGTACCGAATATCGATAAGGCCATTATCTCCGTCCACTGCCACGACGACCTGGGTATGGCGGTCGGCAACT
CCATCGCCGCGGTGCAGGCGGGTGCTCGCCAGGTCGAAGGCACCTTGAACGGCATTGGTGAACGCGCAGGCAATACCTCG
CTGGAAGAAGTGATCATGGCGATCAAAGTGCGCCAGGACATCATGAACGTGCACACCAATATCAATCATCAGGAAATTTT
CCGCACCAGCCAAATCGTCAGCCAGCTGTGCAACATGCCGATCCCGGCCAACAAGGCTATCGTCGGTTCCAATGCCTTCG
CCCACTCTTCTGGCATTCATCAGGACGGCGTGCTGAAAAATCGTGAAAACTACGAAATCATGTCACCGCAGACCATAGGT
CTGAAAGATGTGCAGTTGAACCTGACCTCTCGTTCCGGCCGCGCCGCAGTGAAACACCGTATGGAAGGGATGGGCTACAA
AGAACAGGACTACAACCTGGACAACCTGTACGCCGCCTTCCTGAAGCTGGCCGATAAGAAAGGTCAGGTGTTCGATTACG
ATCTGGAAGCTCTGGCCTTCATTAATAAGCAGCAGGAAGAACCCGAGCATTTCAGCCTGGACTACTTCAGCGTGCAGTCA
GGCAGCAGCATCATGGCCACCGCATCGGTGAAATTGATCTGCGGTGAAGAAGAAAAAGCCGAAGCGGCCACCGGCAACGG
GCCGGTTGACGCGGTCTATCAGGCCATCAACCGCATTACCGGATACCCGATCGAACTGGTGAAATACCAGTTAACCGCCA
AAGGCCAGGGCCGTGACGCACTGGGCCAGGTGGATATCGTGGTGTCCTACAACGGCCGCCGCTTCCACGGCGTGGGTCTG
GCGACCGACATTGTGGAATCCTCTGCCAAGGCGATGGTTCACGTATTAAATAATATTTGGCGCTCCCAGCAGGTAGAAAA
AGAAAAACAGCGTCTGCAGCAAAGCAAACATCAAAATAATCAGGAAACGGTGTGA

Upstream 100 bases:

>100_bases
TTCAGAGCTGATTCAGCCCACCTCATAACAAACCCGCGCTACTGCGCGGGTTTTTTTTTACCCGCTTAGCGTGAAATAAA
CACGATAAGGAACCACCCTG

Downstream 100 bases:

>100_bases
ACATGACGAAGACCTACCACATTGCCGTCTTGCCCGGAGACGGAATCGGCCCGGAAGTAATGGCCCAGGCCCGTAAGGTG
TTGAACGCAGTGCGTCAGCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKIQIAMALERMGVDVMEVGFPVSSPGDFESVQTIARQIKNSRVCGLARCVDKD
IDVAAEALRVAEAFRIHVFLATSTLHIESKLKRSFDEVLEMAIRSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAIN
AGATTINIPDTVGYTTPNQFGGIITTLYDRVPNIDKAIISVHCHDDLGMAVGNSIAAVQAGARQVEGTLNGIGERAGNTS
LEEVIMAIKVRQDIMNVHTNINHQEIFRTSQIVSQLCNMPIPANKAIVGSNAFAHSSGIHQDGVLKNRENYEIMSPQTIG
LKDVQLNLTSRSGRAAVKHRMEGMGYKEQDYNLDNLYAAFLKLADKKGQVFDYDLEALAFINKQQEEPEHFSLDYFSVQS
GSSIMATASVKLICGEEEKAEAATGNGPVDAVYQAINRITGYPIELVKYQLTAKGQGRDALGQVDIVVSYNGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRSQQVEKEKQRLQQSKHQNNQETV

Sequences:

>Translated_524_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKIQIAMALERMGVDVMEVGFPVSSPGDFESVQTIARQIKNSRVCGLARCVDKD
IDVAAEALRVAEAFRIHVFLATSTLHIESKLKRSFDEVLEMAIRSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAIN
AGATTINIPDTVGYTTPNQFGGIITTLYDRVPNIDKAIISVHCHDDLGMAVGNSIAAVQAGARQVEGTLNGIGERAGNTS
LEEVIMAIKVRQDIMNVHTNINHQEIFRTSQIVSQLCNMPIPANKAIVGSNAFAHSSGIHQDGVLKNRENYEIMSPQTIG
LKDVQLNLTSRSGRAAVKHRMEGMGYKEQDYNLDNLYAAFLKLADKKGQVFDYDLEALAFINKQQEEPEHFSLDYFSVQS
GSSIMATASVKLICGEEEKAEAATGNGPVDAVYQAINRITGYPIELVKYQLTAKGQGRDALGQVDIVVSYNGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRSQQVEKEKQRLQQSKHQNNQETV
>Mature_523_residues
SQQVIIFDTTLRDGEQALQASLSVKEKIQIAMALERMGVDVMEVGFPVSSPGDFESVQTIARQIKNSRVCGLARCVDKDI
DVAAEALRVAEAFRIHVFLATSTLHIESKLKRSFDEVLEMAIRSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAINA
GATTINIPDTVGYTTPNQFGGIITTLYDRVPNIDKAIISVHCHDDLGMAVGNSIAAVQAGARQVEGTLNGIGERAGNTSL
EEVIMAIKVRQDIMNVHTNINHQEIFRTSQIVSQLCNMPIPANKAIVGSNAFAHSSGIHQDGVLKNRENYEIMSPQTIGL
KDVQLNLTSRSGRAAVKHRMEGMGYKEQDYNLDNLYAAFLKLADKKGQVFDYDLEALAFINKQQEEPEHFSLDYFSVQSG
SSIMATASVKLICGEEEKAEAATGNGPVDAVYQAINRITGYPIELVKYQLTAKGQGRDALGQVDIVVSYNGRRFHGVGLA
TDIVESSAKAMVHVLNNIWRSQQVEKEKQRLQQSKHQNNQETV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=299, Percent_Identity=23.7458193979933, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1786261, Length=524, Percent_Identity=83.969465648855, Blast_Score=917, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320071, Length=359, Percent_Identity=28.4122562674095, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320019, Length=359, Percent_Identity=28.4122562674095, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=545, Percent_Identity=26.2385321100917, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=548, Percent_Identity=25.9124087591241, Blast_Score=130, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24582381, Length=290, Percent_Identity=24.1379310344828, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_SERP5 (A8G9R1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476979.1
- ProteinModelPortal:   A8G9R1
- SMR:   A8G9R1
- STRING:   A8G9R1
- GeneID:   5604888
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0745
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   SPRO399741:SPRO_0745-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 57647; Mature: 57516

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQVIIFDTTLRDGEQALQASLSVKEKIQIAMALERMGVDVMEVGFPVSSPGDFESVQT
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHH
IARQIKNSRVCGLARCVDKDIDVAAEALRVAEAFRIHVFLATSTLHIESKLKRSFDEVLE
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHH
MAIRSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAINAGATTINIPDTVGYTTPNQF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
GGIITTLYDRVPNIDKAIISVHCHDDLGMAVGNSIAAVQAGARQVEGTLNGIGERAGNTS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LEEVIMAIKVRQDIMNVHTNINHQEIFRTSQIVSQLCNMPIPANKAIVGSNAFAHSSGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGVLKNRENYEIMSPQTIGLKDVQLNLTSRSGRAAVKHRMEGMGYKEQDYNLDNLYAAF
CCCCCCCCCCCEEECCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFINKQQEEPEHFSLDYFSVQSGSSIMATASVKLICGEEEKA
HHHHCCCCCEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEEEEEECCCHHC
EAATGNGPVDAVYQAINRITGYPIELVKYQLTAKGQGRDALGQVDIVVSYNGRRFHGVGL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEEEECCCEEECCCH
ATDIVESSAKAMVHVLNNIWRSQQVEKEKQRLQQSKHQNNQETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SQQVIIFDTTLRDGEQALQASLSVKEKIQIAMALERMGVDVMEVGFPVSSPGDFESVQT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHH
IARQIKNSRVCGLARCVDKDIDVAAEALRVAEAFRIHVFLATSTLHIESKLKRSFDEVLE
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEHHHHHHHHHHHHHHHH
MAIRSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAINAGATTINIPDTVGYTTPNQF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
GGIITTLYDRVPNIDKAIISVHCHDDLGMAVGNSIAAVQAGARQVEGTLNGIGERAGNTS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LEEVIMAIKVRQDIMNVHTNINHQEIFRTSQIVSQLCNMPIPANKAIVGSNAFAHSSGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGVLKNRENYEIMSPQTIGLKDVQLNLTSRSGRAAVKHRMEGMGYKEQDYNLDNLYAAF
CCCCCCCCCCCEEECCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFINKQQEEPEHFSLDYFSVQSGSSIMATASVKLICGEEEKA
HHHHCCCCCEEECCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEEEEEECCCHHC
EAATGNGPVDAVYQAINRITGYPIELVKYQLTAKGQGRDALGQVDIVVSYNGRRFHGVGL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEEEECCCEEECCCH
ATDIVESSAKAMVHVLNNIWRSQQVEKEKQRLQQSKHQNNQETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA