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The map label for this gene is leuB [H]

Identifier: 157368989

GI number: 157368989

Start: 827765

End: 828856

Strand: Reverse

Name: leuB [H]

Synonym: Spro_0744

Alternate gene names: 157368989

Gene position: 828856-827765 (Counterclockwise)

Preceding gene: 157368990

Following gene: 157368988

Centisome position: 15.21

GC content: 59.89

Gene sequence:

>1092_bases
ATGACGAAGACCTACCACATTGCCGTCTTGCCCGGAGACGGAATCGGCCCGGAAGTAATGGCCCAGGCCCGTAAGGTGTT
GAACGCAGTGCGTCAGCGCTTTGATATCCGCATCACTGAAGCCGAGTATGACGTCGGTGGCATCGCCATCGATCGCCATG
GCAGCCCGCTGCCTCCGGCCACCCTCGCCGGTTGCGAGCAGGCCGACGCCATCCTGTTCGGCTCGGTCGGTGGCCCGAAG
TGGGAACACCTGCCGCCGGCAGAGCAGCCTGAACGTGGCGCTTTGCTGCCGTTGCGTAAACACTTCAAACTGTTCAGCAA
CCTGCGCCCTGCCCGTTTGTATCAGGGGCTGGAAGAGTTCTGCCCACTGCGAGCCGATATCGCCGCACGTGGTTTCGATA
TTTTGTGCGTGCGTGAACTGACCGGCGGTATTTACTTTGGCCAGCCTAAAGGCCGCGAAGGCCAGGGCATGCAGGAACGC
GCCTTCGATACCGAGGTGTATCACCGCTTCGAGATCGAGCGCATTGCACGCATCGCCTTTGAGTCTGCCCGCAAGCGTCG
TAACAAAGTGACCTCGATTGATAAGGCCAACGTACTGCAAAGCTCCATTTTGTGGCGCGAAGTGGTTAATCAGGTCGCCC
AGGATTACCCGGACGTGTCGCTGTCACATATGTACATCGACAACGCCACCATGCAGTTGATCAAGGACCCGTCCCAGTTC
GACGTGCTGCTGTGCTCCAACCTGTTCGGCGACATTCTGTCCGACGAATGTGCGATGATCACCGGTTCGATGGGCATGCT
GCCTTCCGCCAGCCTGAACGAACAGGGCTTTGGTCTGTATGAGCCGGCCGGTGGCTCAGCGCCGGATATCGCGGGTAAAG
GCATTGCCAACCCAATCGCCCAGATCCTGTCTGCCACCCTGCTGCTGCGTTACAGCCTGGGCGCCACAGAGGCGGCCGAC
GCCGTTGAACACGCCATCAATCAGGCACTGGAACAAGGTTACCGCACCGCCGATCTGGCCGGTGTCGGCCCAGCCATCAG
CACCGATGAAATGGGTGACATCATCGCCCGCTTTGTCGCTCAGGGGGCATAA

Upstream 100 bases:

>100_bases
TTCACGTATTAAATAATATTTGGCGCTCCCAGCAGGTAGAAAAAGAAAAACAGCGTCTGCAGCAAAGCAAACATCAAAAT
AATCAGGAAACGGTGTGAAC

Downstream 100 bases:

>100_bases
CATGTCTAAAACTTTATATCAGAAATTATACGATGCCCACGTGGTGTACTCAGCGCCGGAAGAAACCCCGCTGTTATATA
TCGATCGCCACCTGGTACAC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTKTYHIAVLPGDGIGPEVMAQARKVLNAVRQRFDIRITEAEYDVGGIAIDRHGSPLPPATLAGCEQADAILFGSVGGPK
WEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEEFCPLRADIAARGFDILCVRELTGGIYFGQPKGREGQGMQER
AFDTEVYHRFEIERIARIAFESARKRRNKVTSIDKANVLQSSILWREVVNQVAQDYPDVSLSHMYIDNATMQLIKDPSQF
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKGIANPIAQILSATLLLRYSLGATEAAD
AVEHAINQALEQGYRTADLAGVGPAISTDEMGDIIARFVAQGA

Sequences:

>Translated_363_residues
MTKTYHIAVLPGDGIGPEVMAQARKVLNAVRQRFDIRITEAEYDVGGIAIDRHGSPLPPATLAGCEQADAILFGSVGGPK
WEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEEFCPLRADIAARGFDILCVRELTGGIYFGQPKGREGQGMQER
AFDTEVYHRFEIERIARIAFESARKRRNKVTSIDKANVLQSSILWREVVNQVAQDYPDVSLSHMYIDNATMQLIKDPSQF
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKGIANPIAQILSATLLLRYSLGATEAAD
AVEHAINQALEQGYRTADLAGVGPAISTDEMGDIIARFVAQGA
>Mature_362_residues
TKTYHIAVLPGDGIGPEVMAQARKVLNAVRQRFDIRITEAEYDVGGIAIDRHGSPLPPATLAGCEQADAILFGSVGGPKW
EHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEEFCPLRADIAARGFDILCVRELTGGIYFGQPKGREGQGMQERA
FDTEVYHRFEIERIARIAFESARKRRNKVTSIDKANVLQSSILWREVVNQVAQDYPDVSLSHMYIDNATMQLIKDPSQFD
VLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKGIANPIAQILSATLLLRYSLGATEAADA
VEHAINQALEQGYRTADLAGVGPAISTDEMGDIIARFVAQGA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=338, Percent_Identity=33.4319526627219, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI28178821, Length=365, Percent_Identity=26.3013698630137, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=28.1150159744409, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4758582, Length=355, Percent_Identity=26.1971830985915, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI28178838, Length=310, Percent_Identity=27.0967741935484, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI28178819, Length=203, Percent_Identity=31.5270935960591, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI87081683, Length=362, Percent_Identity=85.0828729281768, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1788101, Length=369, Percent_Identity=36.5853658536585, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=25.3768844221106, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=33.6158192090396, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17505779, Length=352, Percent_Identity=30.1136363636364, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25144293, Length=348, Percent_Identity=28.1609195402299, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=365, Percent_Identity=27.3972602739726, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=372, Percent_Identity=42.4731182795699, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=369, Percent_Identity=33.0623306233062, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=30.8988764044944, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=362, Percent_Identity=29.0055248618785, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=33.4310850439883, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=33.4310850439883, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24661184, Length=344, Percent_Identity=29.9418604651163, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161078633, Length=361, Percent_Identity=30.1939058171745, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24650122, Length=361, Percent_Identity=30.1939058171745, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI161078637, Length=361, Percent_Identity=30.1939058171745, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI161078635, Length=361, Percent_Identity=30.1939058171745, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI161078639, Length=359, Percent_Identity=30.3621169916435, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281362242, Length=342, Percent_Identity=26.6081871345029, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648872, Length=342, Percent_Identity=26.6081871345029, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI20130355, Length=343, Percent_Identity=23.6151603498542, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39455; Mature: 39324

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTYHIAVLPGDGIGPEVMAQARKVLNAVRQRFDIRITEAEYDVGGIAIDRHGSPLPPA
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEECCCCCCCCH
TLAGCEQADAILFGSVGGPKWEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEEF
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
CPLRADIAARGFDILCVRELTGGIYFGQPKGREGQGMQERAFDTEVYHRFEIERIARIAF
CCCHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ESARKRRNKVTSIDKANVLQSSILWREVVNQVAQDYPDVSLSHMYIDNATMQLIKDPSQF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCHH
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKGIANPIA
HHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHH
QILSATLLLRYSLGATEAADAVEHAINQALEQGYRTADLAGVGPAISTDEMGDIIARFVA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QGA
CCC
>Mature Secondary Structure 
TKTYHIAVLPGDGIGPEVMAQARKVLNAVRQRFDIRITEAEYDVGGIAIDRHGSPLPPA
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEECCCCCCCCH
TLAGCEQADAILFGSVGGPKWEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEEF
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
CPLRADIAARGFDILCVRELTGGIYFGQPKGREGQGMQERAFDTEVYHRFEIERIARIAF
CCCHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ESARKRRNKVTSIDKANVLQSSILWREVVNQVAQDYPDVSLSHMYIDNATMQLIKDPSQF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCHH
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKGIANPIA
HHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHH
QILSATLLLRYSLGATEAADAVEHAINQALEQGYRTADLAGVGPAISTDEMGDIIARFVA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QGA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]