Click here to switch to the map view.

The map label for this gene is leuC [H]

Identifier: 157368988

GI number: 157368988

Start: 826363

End: 827763

Strand: Reverse

Name: leuC [H]

Synonym: Spro_0743

Alternate gene names: 157368988

Gene position: 827763-826363 (Counterclockwise)

Preceding gene: 157368989

Following gene: 157368987

Centisome position: 15.19

GC content: 59.1

Gene sequence:

>1401_bases
ATGTCTAAAACTTTATATCAGAAATTATACGATGCCCACGTGGTGTACTCAGCGCCGGAAGAAACCCCGCTGTTATATAT
CGATCGCCACCTGGTACACGAAGTGACTTCGCCTCAGGCATTCGATGGCCTGCGCGCCATGGGCCGCAAAGTGCGCCAGC
CGGGCAAGACTTTTGCCACCATGGACCACAACGTCTCTACCCAAACCAAAGACATCAACGCCAGCGGCGAGATGGCACGC
ATTCAGATGCAGGAACTGATCAAGAACTGCGAAGAGTTTGGCGTATTGCTGTATGACCTGAACCACCCATACCAGGGCAT
CGTACACGTGATCGGCCCAGAGCAGGGCATGACACTGCCGGGTATGACCATCGTCTGCGGCGACTCCCACACCGCGACTC
ACGGGGCTTTCGGTTCGCTGGCGTTTGGTATCGGCACCTCCGAAGTGGAGCACGTGTTGGCGACCCAAACCCTGAAGCAG
GGCCGCGCCAAGACCATGAAAATTGAAGTGACCGGCGACGCCGCGGAAGGCATCACGGCGAAAGACATCGTACTGGCGGT
GATCGGCAAAACCGGCAGCGCCGGCGGCACCGGCCACGTAGTGGAATTCTGCGGCAAGGCCATTCAGGCGTTGAGCATGG
AAGGCCGCATGACGCTGTGCAACATGGCGATCGAAATGGGTGCCAAAGCCGGGCTGGTTGCGCCGGACGACACCACCTTT
GATTACCTGAAGGGCCGTCAGTTTGCGCCAACCGGCAGCAACTGGGAACAGGCCGTTGCCTACTGGCGCACGCTGAAATC
CGATGACGATGCCAAATTCGATACCGTTGTCACCCTGCGTGCTGAAGACATCGCGCCACAGGTGACCTGGGGAACCAACC
CTGGTCAGGTTATCGCCGTCAACCAGACCATCCCGGCCCCTGAGTCGTTCAGCGATCCGGGTGAGCGTGCCTCGGCCGAA
AAAGCCCTGGCCTATATGGACCTGAAACCGGGCATCAAACTGACAGAAGTGCCGATCGACAAAGTCTTTATCGGTTCCTG
CACCAACTCACGCATTGAAGACTTGCGTGCAGCGGCTGCCATCGCCAAGGGCCGCAAGGTTGCCAGCGGTGTTCAGGCCA
TCGTGGTACCCGGCTCCGGCCCGGTAAAAGCGCAGGCGGAAGCCGAAGGCCTGGATAAAATCTTTATCGATGCCGGTTTT
GAATGGCGCCTGCCGGGCTGCTCAATGTGTCTGGCGATGAACAACGACCGCCTGAACCCCGGCGAACGCTGTGCCTCCAC
CAGCAACCGTAACTTTGAAGGGCGTCAGGGCCGCGGTGGGCGTACTCACCTGGTCAGCCCGGCAATGGCCGCTGCGGCCG
CTATTGCCGGCCATTTCGCCGATATCCGTGATATTCACTAA

Upstream 100 bases:

>100_bases
CAAGGTTACCGCACCGCCGATCTGGCCGGTGTCGGCCCAGCCATCAGCACCGATGAAATGGGTGACATCATCGCCCGCTT
TGTCGCTCAGGGGGCATAAC

Downstream 100 bases:

>100_bases
GGAAGGCTACCGTGGCTAAATTTACTCAACATACCGGCTTAGTGGTGCCTTTGGATGCGGCGAACGTCGATACCGATGCC
ATTATTCCAAAACAGTTTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSKTLYQKLYDAHVVYSAPEETPLLYIDRHLVHEVTSPQAFDGLRAMGRKVRQPGKTFATMDHNVSTQTKDINASGEMAR
IQMQELIKNCEEFGVLLYDLNHPYQGIVHVIGPEQGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVTGDAAEGITAKDIVLAVIGKTGSAGGTGHVVEFCGKAIQALSMEGRMTLCNMAIEMGAKAGLVAPDDTTF
DYLKGRQFAPTGSNWEQAVAYWRTLKSDDDAKFDTVVTLRAEDIAPQVTWGTNPGQVIAVNQTIPAPESFSDPGERASAE
KALAYMDLKPGIKLTEVPIDKVFIGSCTNSRIEDLRAAAAIAKGRKVASGVQAIVVPGSGPVKAQAEAEGLDKIFIDAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIAGHFADIRDIH

Sequences:

>Translated_466_residues
MSKTLYQKLYDAHVVYSAPEETPLLYIDRHLVHEVTSPQAFDGLRAMGRKVRQPGKTFATMDHNVSTQTKDINASGEMAR
IQMQELIKNCEEFGVLLYDLNHPYQGIVHVIGPEQGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQ
GRAKTMKIEVTGDAAEGITAKDIVLAVIGKTGSAGGTGHVVEFCGKAIQALSMEGRMTLCNMAIEMGAKAGLVAPDDTTF
DYLKGRQFAPTGSNWEQAVAYWRTLKSDDDAKFDTVVTLRAEDIAPQVTWGTNPGQVIAVNQTIPAPESFSDPGERASAE
KALAYMDLKPGIKLTEVPIDKVFIGSCTNSRIEDLRAAAAIAKGRKVASGVQAIVVPGSGPVKAQAEAEGLDKIFIDAGF
EWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIAGHFADIRDIH
>Mature_465_residues
SKTLYQKLYDAHVVYSAPEETPLLYIDRHLVHEVTSPQAFDGLRAMGRKVRQPGKTFATMDHNVSTQTKDINASGEMARI
QMQELIKNCEEFGVLLYDLNHPYQGIVHVIGPEQGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQG
RAKTMKIEVTGDAAEGITAKDIVLAVIGKTGSAGGTGHVVEFCGKAIQALSMEGRMTLCNMAIEMGAKAGLVAPDDTTFD
YLKGRQFAPTGSNWEQAVAYWRTLKSDDDAKFDTVVTLRAEDIAPQVTWGTNPGQVIAVNQTIPAPESFSDPGERASAEK
ALAYMDLKPGIKLTEVPIDKVFIGSCTNSRIEDLRAAAAIAKGRKVASGVQAIVVPGSGPVKAQAEAEGLDKIFIDAGFE
WRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIAGHFADIRDIH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=410, Percent_Identity=26.8292682926829, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI8659555, Length=448, Percent_Identity=25.8928571428571, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI41352693, Length=381, Percent_Identity=25.9842519685039, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=87.5268817204301, Blast_Score=837, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=487, Percent_Identity=26.8993839835729, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI87081781, Length=371, Percent_Identity=24.7978436657682, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=412, Percent_Identity=28.8834951456311, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=412, Percent_Identity=28.8834951456311, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=30.2931596091205, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=448, Percent_Identity=25.8928571428571, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=59.6602972399151, Blast_Score=574, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6320440, Length=424, Percent_Identity=27.5943396226415, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=367, Percent_Identity=29.700272479564, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=405, Percent_Identity=26.1728395061728, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI281365315, Length=447, Percent_Identity=26.8456375838926, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17864292, Length=447, Percent_Identity=26.8456375838926, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161076999, Length=409, Percent_Identity=27.1393643031785, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571643, Length=411, Percent_Identity=26.7639902676399, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645686, Length=450, Percent_Identity=26, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17137564, Length=476, Percent_Identity=24.5798319327731, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49888; Mature: 49757

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTLYQKLYDAHVVYSAPEETPLLYIDRHLVHEVTSPQAFDGLRAMGRKVRQPGKTFAT
CCHHHHHHHHCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
MDHNVSTQTKDINASGEMARIQMQELIKNCEEFGVLLYDLNHPYQGIVHVIGPEQGMTLP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGDAAEGITA
CEEEEECCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAVIGKTGSAGGTGHVVEFCGKAIQALSMEGRMTLCNMAIEMGAKAGLVAPDDTTF
HHEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCH
DYLKGRQFAPTGSNWEQAVAYWRTLKSDDDAKFDTVVTLRAEDIAPQVTWGTNPGQVIAV
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCEEEECCCCCEEEEE
NQTIPAPESFSDPGERASAEKALAYMDLKPGIKLTEVPIDKVFIGSCTNSRIEDLRAAAA
ECCCCCCCCCCCCCCHHHHHHHEEEEECCCCCEEEECCCCEEEEECCCCHHHHHHHHHHH
IAKGRKVASGVQAIVVPGSGPVKAQAEAEGLDKIFIDAGFEWRLPGCSMCLAMNNDRLNP
HHCCCHHHCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIAGHFADIRDIH
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKTLYQKLYDAHVVYSAPEETPLLYIDRHLVHEVTSPQAFDGLRAMGRKVRQPGKTFAT
CHHHHHHHHCCEEEEECCCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
MDHNVSTQTKDINASGEMARIQMQELIKNCEEFGVLLYDLNHPYQGIVHVIGPEQGMTLP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGDAAEGITA
CEEEEECCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCH
KDIVLAVIGKTGSAGGTGHVVEFCGKAIQALSMEGRMTLCNMAIEMGAKAGLVAPDDTTF
HHEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCH
DYLKGRQFAPTGSNWEQAVAYWRTLKSDDDAKFDTVVTLRAEDIAPQVTWGTNPGQVIAV
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCEEEECCCCCEEEEE
NQTIPAPESFSDPGERASAEKALAYMDLKPGIKLTEVPIDKVFIGSCTNSRIEDLRAAAA
ECCCCCCCCCCCCCCHHHHHHHEEEEECCCCCEEEECCCCEEEEECCCCHHHHHHHHHHH
IAKGRKVASGVQAIVVPGSGPVKAQAEAEGLDKIFIDAGFEWRLPGCSMCLAMNNDRLNP
HHCCCHHHCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCH
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIAGHFADIRDIH
HHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA