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The map label for this gene is pyrB

Identifier: 157368790

GI number: 157368790

Start: 603055

End: 603990

Strand: Reverse

Name: pyrB

Synonym: Spro_0545

Alternate gene names: 157368790

Gene position: 603990-603055 (Counterclockwise)

Preceding gene: 157368791

Following gene: 157368789

Centisome position: 11.08

GC content: 59.72

Gene sequence:

>936_bases
ATGGCCAACCCGCTGTATCACAAACATATCATCTCTATTAACGATCTCAGCCGCGAGGATCTGGAGCTGGTGCTGCGCAC
CGCCGCCAGCCTGAAAGCCAATCCGCAGCCCGAGCTGCTAAAGCACAAGGTGATCGCCAGCTGCTTCTTTGAAGCCTCCA
CCCGTACCCGCCTGTCGTTTGAAACCTCGATGCACCGTCTGGGCGCTTCGGTCGTGGGCTTTGCCGACGGCAGCAATACC
TCGCTCGGCAAGAAAGGCGAAACCCTGGCCGACACCATCTCGGTGATTGGTACCTACGTCGATGCCATCGTGATGCGTCA
CCCGCAGGAAGGCGCGGCGCGGCTGGCGACCGAGTTCTCCGGCGGCATTCCGGTGCTCAACGCCGGTGACGGTGCCAACC
AGCACCCGACCCAGACGCTGCTCGACCTGTTCACCATTCAGGAAACCCAAGGCCGCCTGAACAACATCAATATCGCCATG
GTCGGCGACCTGAAATACGGCCGCACCGTACACTCGCTGGCGCAGGCGCTGGCCAAGTTTGAAGGCAACCGCTTCTTCTT
CATCGCCCCGGACGCGCTGGCGATGCCGGCCTACATCCTGCAGATGCTGGAAGAGAAAGGTATTCAATACAGCCTGCACA
GCAGCATTGAAGAAGTGGTGCCGGAGCTGGACATTCTCTACATGACCCGGGTGCAGAAAGAGCGCCTGGATCCTTCCGAA
TACGCCAACGTGAAGGCGCAGTTCGTACTGCGCGCGGCAGATCTGGCCGGGGCACGTGACAACCTCAAGGTGCTGCACCC
GCTGCCGCGCATTGATGAGATCACCACCGACGTGGATAAAACGCCGTACGCCTACTACTTCCAGCAGGCGGGCAACGGTA
TTTTCGCCCGTCAGGCGCTGTTGGCGCTGGTATTAAACGCAGATTTGGCTCTTTAA

Upstream 100 bases:

>100_bases
TTCTCCTGTTGCTAAACCGATCGTTTCAAGCCCCTCGTTGAGGGGCTTTTTTTTGCCTGAAAAGTCCCGCGCCACGGGCA
CGAGTCGAGGAGAGTGAAAA

Downstream 100 bases:

>100_bases
GGGGGAACCGTCATGACTCACGATAACAAACTGCAGGTTGAAGCGATCAAGTGCGGTACGGTGATTGACCATATTCCGGC
GCAGATAGGTTTTAAGCTGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MANPLYHKHIISINDLSREDLELVLRTAASLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFADGSNT
SLGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFSGGIPVLNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAM
VGDLKYGRTVHSLAQALAKFEGNRFFFIAPDALAMPAYILQMLEEKGIQYSLHSSIEEVVPELDILYMTRVQKERLDPSE
YANVKAQFVLRAADLAGARDNLKVLHPLPRIDEITTDVDKTPYAYYFQQAGNGIFARQALLALVLNADLAL

Sequences:

>Translated_311_residues
MANPLYHKHIISINDLSREDLELVLRTAASLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFADGSNT
SLGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFSGGIPVLNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAM
VGDLKYGRTVHSLAQALAKFEGNRFFFIAPDALAMPAYILQMLEEKGIQYSLHSSIEEVVPELDILYMTRVQKERLDPSE
YANVKAQFVLRAADLAGARDNLKVLHPLPRIDEITTDVDKTPYAYYFQQAGNGIFARQALLALVLNADLAL
>Mature_310_residues
ANPLYHKHIISINDLSREDLELVLRTAASLKANPQPELLKHKVIASCFFEASTRTRLSFETSMHRLGASVVGFADGSNTS
LGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFSGGIPVLNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAMV
GDLKYGRTVHSLAQALAKFEGNRFFFIAPDALAMPAYILQMLEEKGIQYSLHSSIEEVVPELDILYMTRVQKERLDPSEY
ANVKAQFVLRAADLAGARDNLKVLHPLPRIDEITTDVDKTPYAYYFQQAGNGIFARQALLALVLNADLAL

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=43.4504792332268, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI38788445, Length=313, Percent_Identity=26.8370607028754, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=87.459807073955, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1786469, Length=298, Percent_Identity=30.5369127516779, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790703, Length=234, Percent_Identity=32.0512820512821, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=324, Percent_Identity=38.8888888888889, Blast_Score=207, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=41.2698412698413, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24642586, Length=316, Percent_Identity=42.4050632911392, Blast_Score=249, Evalue=1e-66,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_SERP5 (A8G961)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476779.1
- ProteinModelPortal:   A8G961
- SMR:   A8G961
- STRING:   A8G961
- GeneID:   5606811
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0545
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- ProtClustDB:   PRK00856
- BioCyc:   SPRO399741:SPRO_0545-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34210; Mature: 34078

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00097 CARBAMOYLTRANSFERASE; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPLYHKHIISINDLSREDLELVLRTAASLKANPQPELLKHKVIASCFFEASTRTRLSF
CCCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEHH
ETSMHRLGASVVGFADGSNTSLGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFS
HHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GGIPVLNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAMVGDLKYGRTVHSLAQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
EGNRFFFIAPDALAMPAYILQMLEEKGIQYSLHSSIEEVVPELDILYMTRVQKERLDPSE
CCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
YANVKAQFVLRAADLAGARDNLKVLHPLPRIDEITTDVDKTPYAYYFQQAGNGIFARQAL
HHCCHHHHHEEHHHHCCCCCCCCEECCCCCHHHHHCCCCCCCHHHHHHHCCCCHHHHHHH
LALVLNADLAL
HHHHHHHCCCC
>Mature Secondary Structure 
ANPLYHKHIISINDLSREDLELVLRTAASLKANPQPELLKHKVIASCFFEASTRTRLSF
CCCCCHHEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEHH
ETSMHRLGASVVGFADGSNTSLGKKGETLADTISVIGTYVDAIVMRHPQEGAARLATEFS
HHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GGIPVLNAGDGANQHPTQTLLDLFTIQETQGRLNNINIAMVGDLKYGRTVHSLAQALAKF
CCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
EGNRFFFIAPDALAMPAYILQMLEEKGIQYSLHSSIEEVVPELDILYMTRVQKERLDPSE
CCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
YANVKAQFVLRAADLAGARDNLKVLHPLPRIDEITTDVDKTPYAYYFQQAGNGIFARQAL
HHCCHHHHHEEHHHHCCCCCCCCEECCCCCHHHHHCCCCCCCHHHHHHHCCCCHHHHHHH
LALVLNADLAL
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA