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The map label for this gene is pyrI [H]

Identifier: 157368789

GI number: 157368789

Start: 602578

End: 603042

Strand: Reverse

Name: pyrI [H]

Synonym: Spro_0544

Alternate gene names: 157368789

Gene position: 603042-602578 (Counterclockwise)

Preceding gene: 157368790

Following gene: 157368788

Centisome position: 11.07

GC content: 55.05

Gene sequence:

>465_bases
ATGACTCACGATAACAAACTGCAGGTTGAAGCGATCAAGTGCGGTACGGTGATTGACCATATTCCGGCGCAGATAGGTTT
TAAGCTGCTGACGCTGTTTAAGCTGACCGCCACCGACCAGCGTATTACCATCGGCCTGAACCTGCCTTCAGGCGAGCTGG
GCCGCAAAGACCTGATCAAAATCGAGAACACTTTCCTGACCGAACAGCAGGCCAACCAACTGGCGATGTATGCGCCCAAA
GCCACGGTCAACCGTATCGACAATTACGAAGTGGTGCGCAAACTGACCCTCAGCCTGCCGGACCATATCGACGGCGTGCT
GACCTGCCCGAACAGCAACTGCATCAGCCGCAGTGAGCCGGTGTCCTCCAGCTTTAGCGTGAAATCCCGCGACGGCGACG
TACATCTGAAATGCCGCTACTGTGAGAAAGAGTTCGAACATCAGGTGGTGTTGCAGGCAGACTGA

Upstream 100 bases:

>100_bases
CGCCTACTACTTCCAGCAGGCGGGCAACGGTATTTTCGCCCGTCAGGCGCTGTTGGCGCTGGTATTAAACGCAGATTTGG
CTCTTTAAGGGGGAACCGTC

Downstream 100 bases:

>100_bases
GTCTGCGGTTTTCGCCGGGAGACGGCGCTGTTATTCTCCCGGCGACGCCCTATAATGGGGTTTCTGGATAGAACAACCCT
CGATTATCAGGAGAAAAAAT

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTHDNKLQVEAIKCGTVIDHIPAQIGFKLLTLFKLTATDQRITIGLNLPSGELGRKDLIKIENTFLTEQQANQLAMYAPK
ATVNRIDNYEVVRKLTLSLPDHIDGVLTCPNSNCISRSEPVSSSFSVKSRDGDVHLKCRYCEKEFEHQVVLQAD

Sequences:

>Translated_154_residues
MTHDNKLQVEAIKCGTVIDHIPAQIGFKLLTLFKLTATDQRITIGLNLPSGELGRKDLIKIENTFLTEQQANQLAMYAPK
ATVNRIDNYEVVRKLTLSLPDHIDGVLTCPNSNCISRSEPVSSSFSVKSRDGDVHLKCRYCEKEFEHQVVLQAD
>Mature_153_residues
THDNKLQVEAIKCGTVIDHIPAQIGFKLLTLFKLTATDQRITIGLNLPSGELGRKDLIKIENTFLTEQQANQLAMYAPKA
TVNRIDNYEVVRKLTLSLPDHIDGVLTCPNSNCISRSEPVSSSFSVKSRDGDVHLKCRYCEKEFEHQVVLQAD

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase [H]

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family [H]

Homologues:

Organism=Escherichia coli, GI1790692, Length=151, Percent_Identity=80.1324503311258, Blast_Score=254, Evalue=2e-69,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- ProDom:   PD006194 [H]

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C [H]

EC number: 2.1.3.2

Molecular weight: Translated: 17254; Mature: 17123

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDNKLQVEAIKCGTVIDHIPAQIGFKLLTLFKLTATDQRITIGLNLPSGELGRKDLIK
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCCCEEE
IENTFLTEQQANQLAMYAPKATVNRIDNYEVVRKLTLSLPDHIDGVLTCPNSNCISRSEP
EHHHEECCHHCCEEEEECCCHHHHCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
VSSSFSVKSRDGDVHLKCRYCEKEFEHQVVLQAD
CCCCCEEECCCCCEEEEEEECCCCCCCEEEEEEC
>Mature Secondary Structure 
THDNKLQVEAIKCGTVIDHIPAQIGFKLLTLFKLTATDQRITIGLNLPSGELGRKDLIK
CCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCCCEEE
IENTFLTEQQANQLAMYAPKATVNRIDNYEVVRKLTLSLPDHIDGVLTCPNSNCISRSEP
EHHHEECCHHCCEEEEECCCHHHHCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
VSSSFSVKSRDGDVHLKCRYCEKEFEHQVVLQAD
CCCCCEEECCCCCEEEEEEECCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2674139 [H]