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The map label for this gene is miaA

Identifier: 157368677

GI number: 157368677

Start: 482113

End: 483054

Strand: Direct

Name: miaA

Synonym: Spro_0430

Alternate gene names: 157368677

Gene position: 482113-483054 (Clockwise)

Preceding gene: 157368676

Following gene: 157368678

Centisome position: 8.85

GC content: 54.56

Gene sequence:

>942_bases
ATGATTGAAACTGAAATGACACCACGTCCCCCGGCTATCTTTATCATGGGGCCGACCGCCTCGGGCAAAACCGCATTGGC
GATCGCGCTGCGTGAACGCCTGCCTGTGGAATTAATCAGCGTGGATTCCGCACTGATTTATCGCGGCATGGATATCGGCA
CGGCCAAACCGAGCGCTGAAGAACTGGCGCAGGCACCGCATCGGCTGATTGATATTCGCGATCCCGCCGAAGCCTACTCC
GCCGCGGACTTTCGCGCCGACGCACTGAAAGAGATGGCCGCCATTACCGCCGCCGGGCGTATCCCGCTGCTGGTGGGGGG
AACCATGCTTTATTTCAAGGCGTTGCTGGAAGGGTTATCGCCGTTGCCACCTGCGGATCCTGCGGTGCGCGAACGCATCG
AACAGCAGGCGGCGGAGCAGGGTTGGGACGTTTTACACCGTCAGTTGCAAGAGATTGACCCGGTTGCGGCATTGAGAATT
CATCCGAATGATCCGCAGAGACTGTCCAGAGCACTGGAAGTTTTTTTTATTTCGGGTAAAACTTTAACGGAACTGACTAA
AATTTCGGGTGAATCGTTGCCGTATCATGTGCACCAATTTGCGATAGCGCCGACCAGCCGTGAGTTGATCCATCAACGCA
TCGAGCTGCGGTACCATCAAATGTTGGCGGCAGGTTTTGAGACGGAAGCGCGTGCACTTTTTGCACGGGGTGATTTGCAT
ACGGACTTGCCTTCCATTCGCTGTGTCGGTTATCGCCAGATGTGGTCATATTTGTCTGGTGAAATTAGTTACGATGAGAT
GGTTTATCGTGGTATTTGCGCAACGCGTCAATTGGCCAAGCGCCAAATGACCTGGTTACGAGGCTGGGACTCGGTACATT
GGCTGGACAGCGAGAAGCCGGGAGAGGCTTTGGACTCGGTAATACAGGTTGTTAGTGCATAG

Upstream 100 bases:

>100_bases
CTGACCGACGTTGAACGACTTTGCCCGCAGCTGGTCAAATCACCACCCAGCGGACTTTTACAACCTGTTGATTTACAGGC
TGCACTGACAGCACTTAGGC

Downstream 100 bases:

>100_bases
GTTGAGTGATTGTGTACAATTGATGAGTATTCAGCGCGCAAATTTTTTAGGTCGTTTATTTTAGAGCCGACAGGTTCTTA
GTTAAAAACAACAAGCAAAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIETEMTPRPPAIFIMGPTASGKTALAIALRERLPVELISVDSALIYRGMDIGTAKPSAEELAQAPHRLIDIRDPAEAYS
AADFRADALKEMAAITAAGRIPLLVGGTMLYFKALLEGLSPLPPADPAVRERIEQQAAEQGWDVLHRQLQEIDPVAALRI
HPNDPQRLSRALEVFFISGKTLTELTKISGESLPYHVHQFAIAPTSRELIHQRIELRYHQMLAAGFETEARALFARGDLH
TDLPSIRCVGYRQMWSYLSGEISYDEMVYRGICATRQLAKRQMTWLRGWDSVHWLDSEKPGEALDSVIQVVSA

Sequences:

>Translated_313_residues
MIETEMTPRPPAIFIMGPTASGKTALAIALRERLPVELISVDSALIYRGMDIGTAKPSAEELAQAPHRLIDIRDPAEAYS
AADFRADALKEMAAITAAGRIPLLVGGTMLYFKALLEGLSPLPPADPAVRERIEQQAAEQGWDVLHRQLQEIDPVAALRI
HPNDPQRLSRALEVFFISGKTLTELTKISGESLPYHVHQFAIAPTSRELIHQRIELRYHQMLAAGFETEARALFARGDLH
TDLPSIRCVGYRQMWSYLSGEISYDEMVYRGICATRQLAKRQMTWLRGWDSVHWLDSEKPGEALDSVIQVVSA
>Mature_313_residues
MIETEMTPRPPAIFIMGPTASGKTALAIALRERLPVELISVDSALIYRGMDIGTAKPSAEELAQAPHRLIDIRDPAEAYS
AADFRADALKEMAAITAAGRIPLLVGGTMLYFKALLEGLSPLPPADPAVRERIEQQAAEQGWDVLHRQLQEIDPVAALRI
HPNDPQRLSRALEVFFISGKTLTELTKISGESLPYHVHQFAIAPTSRELIHQRIELRYHQMLAAGFETEARALFARGDLH
TDLPSIRCVGYRQMWSYLSGEISYDEMVYRGICATRQLAKRQMTWLRGWDSVHWLDSEKPGEALDSVIQVVSA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=316, Percent_Identity=28.4810126582279, Blast_Score=122, Evalue=3e-28,
Organism=Escherichia coli, GI1790613, Length=304, Percent_Identity=85.1973684210526, Blast_Score=539, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25144712, Length=304, Percent_Identity=25.9868421052632, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=26.3888888888889, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24649830, Length=285, Percent_Identity=30.1754385964912, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SERP5 (A8G8U8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476666.1
- ProteinModelPortal:   A8G8U8
- SMR:   A8G8U8
- STRING:   A8G8U8
- GeneID:   5603684
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0430
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   LPYTVAN
- ProtClustDB:   PRK00091
- BioCyc:   SPRO399741:SPRO_0430-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34877; Mature: 34877

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETEMTPRPPAIFIMGPTASGKTALAIALRERLPVELISVDSALIYRGMDIGTAKPSAE
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCCCHH
ELAQAPHRLIDIRDPAEAYSAADFRADALKEMAAITAAGRIPLLVGGTMLYFKALLEGLS
HHHHCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCC
PLPPADPAVRERIEQQAAEQGWDVLHRQLQEIDPVAALRIHPNDPQRLSRALEVFFISGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHEECCC
TLTELTKISGESLPYHVHQFAIAPTSRELIHQRIELRYHQMLAAGFETEARALFARGDLH
HHHHHHHHCCCCCCEEHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
TDLPSIRCVGYRQMWSYLSGEISYDEMVYRGICATRQLAKRQMTWLRGWDSVHWLDSEKP
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
GEALDSVIQVVSA
HHHHHHHHHHHCC
>Mature Secondary Structure
MIETEMTPRPPAIFIMGPTASGKTALAIALRERLPVELISVDSALIYRGMDIGTAKPSAE
CCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCCCHH
ELAQAPHRLIDIRDPAEAYSAADFRADALKEMAAITAAGRIPLLVGGTMLYFKALLEGLS
HHHHCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCC
PLPPADPAVRERIEQQAAEQGWDVLHRQLQEIDPVAALRIHPNDPQRLSRALEVFFISGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHEECCC
TLTELTKISGESLPYHVHQFAIAPTSRELIHQRIELRYHQMLAAGFETEARALFARGDLH
HHHHHHHHCCCCCCEEHHHEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
TDLPSIRCVGYRQMWSYLSGEISYDEMVYRGICATRQLAKRQMTWLRGWDSVHWLDSEKP
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
GEALDSVIQVVSA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA