Click here to switch to the map view.

The map label for this gene is nfi

Identifier: 157368537

GI number: 157368537

Start: 330397

End: 331083

Strand: Direct

Name: nfi

Synonym: Spro_0288

Alternate gene names: 157368537

Gene position: 330397-331083 (Clockwise)

Preceding gene: 157368536

Following gene: 157368538

Centisome position: 6.06

GC content: 62.59

Gene sequence:

>687_bases
GTGATAGATACTCAAGCCCTGCGTGCAGAACAACGGCTGCGCGCCTCCGAAGTCATTCGCCAGGATGATTTCCTGGTCGT
TCCACCGGCCTTTATCGCCGGTGCCGACGTGGGCTTTGAGCAGGAGGGCGCGGTGACTCGCGCCGCTATCGCCATCCTGC
GCTATCCGTCGCTGGAGCTGGTGGAATACCAGGTGGCACGAGTGGCCACCACCATGCCCTATATCCCCGGTTTCTTATCG
TTCCGTGAATACCCAGCGTTGCTCGCAGCCTGGGAGCAACTGCATCAAAAGCCGGATCTGGTGCTGGTTGACGGCCATGG
CATTTCGCATCCGCGCCGTCTGGGCGTAGCCAGCCATTTTGGCCTGCTGGTCAACGTGCCGACCATTGGCGTAGCCAAAA
AGCGCCTGTGCGGTAAGTTCGCCCCGCTGGACGAGGCTACAGATGCGCTGGCACCGCTGGAGGACAAAGGCGAGCAACTG
GGTTGGGTATGGCGCAGTAAGGCGCGCTGTAATCCGTTGTTTATCTCCACCGGCCATCGCGTTGGCGCCGACAGTGCACT
GGCGTGGGTGCAACGTTGCATGGCGGGCTACCGTTTGCCGGAGCCTACACGCTGGGCCGACGCCATCGCCTCGCGCCGCC
CGGCCTTCCAGCGCTGGCTGCAGCAGCATCCAGAGGTGTCGCAATGA

Upstream 100 bases:

>100_bases
GGGCCACGGTATTCACCCGGACGTGCCGCCGGAAAACGCCGGTGCCTTTGTTGAGGCAGTCCACGCGCAGTCGGGGAAAT
ACCATCGTTAAGGATTGCAT

Downstream 100 bases:

>100_bases
CCGATTTCGGGTACACTGCGGCGCAGATAACGCGTAAGAGAGTGAATAATGCTACGTAACCCGATTCATTTACGTCTGGA
AAAGCTTGAAAGCTGGCAAC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MIDTQALRAEQRLRASEVIRQDDFLVVPPAFIAGADVGFEQEGAVTRAAIAILRYPSLELVEYQVARVATTMPYIPGFLS
FREYPALLAAWEQLHQKPDLVLVDGHGISHPRRLGVASHFGLLVNVPTIGVAKKRLCGKFAPLDEATDALAPLEDKGEQL
GWVWRSKARCNPLFISTGHRVGADSALAWVQRCMAGYRLPEPTRWADAIASRRPAFQRWLQQHPEVSQ

Sequences:

>Translated_228_residues
MIDTQALRAEQRLRASEVIRQDDFLVVPPAFIAGADVGFEQEGAVTRAAIAILRYPSLELVEYQVARVATTMPYIPGFLS
FREYPALLAAWEQLHQKPDLVLVDGHGISHPRRLGVASHFGLLVNVPTIGVAKKRLCGKFAPLDEATDALAPLEDKGEQL
GWVWRSKARCNPLFISTGHRVGADSALAWVQRCMAGYRLPEPTRWADAIASRRPAFQRWLQQHPEVSQ
>Mature_228_residues
MIDTQALRAEQRLRASEVIRQDDFLVVPPAFIAGADVGFEQEGAVTRAAIAILRYPSLELVEYQVARVATTMPYIPGFLS
FREYPALLAAWEQLHQKPDLVLVDGHGISHPRRLGVASHFGLLVNVPTIGVAKKRLCGKFAPLDEATDALAPLEDKGEQL
GWVWRSKARCNPLFISTGHRVGADSALAWVQRCMAGYRLPEPTRWADAIASRRPAFQRWLQQHPEVSQ

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=228, Percent_Identity=38.1578947368421, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI257467548, Length=223, Percent_Identity=30.9417040358744, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI257467550, Length=223, Percent_Identity=30.9417040358744, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI87082357, Length=223, Percent_Identity=78.0269058295964, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_SERP5 (A8G8F8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476526.1
- ProteinModelPortal:   A8G8F8
- STRING:   A8G8F8
- GeneID:   5607064
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0288
- eggNOG:   COG1515
- HOGENOM:   HBG716360
- OMA:   PTRRAHE
- ProtClustDB:   PRK11617
- BioCyc:   SPRO399741:SPRO_0288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 25386; Mature: 25386

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTQALRAEQRLRASEVIRQDDFLVVPPAFIAGADVGFEQEGAVTRAAIAILRYPSLEL
CCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHH
VEYQVARVATTMPYIPGFLSFREYPALLAAWEQLHQKPDLVLVDGHGISHPRRLGVASHF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCHHHHC
GLLVNVPTIGVAKKRLCGKFAPLDEATDALAPLEDKGEQLGWVWRSKARCNPLFISTGHR
CEEEECCCCHHHHHHHHHCCCCCHHHHHHHCCCHHCCCHHHHHHHCCCCCCEEEEECCCC
VGADSALAWVQRCMAGYRLPEPTRWADAIASRRPAFQRWLQQHPEVSQ
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIDTQALRAEQRLRASEVIRQDDFLVVPPAFIAGADVGFEQEGAVTRAAIAILRYPSLEL
CCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHH
VEYQVARVATTMPYIPGFLSFREYPALLAAWEQLHQKPDLVLVDGHGISHPRRLGVASHF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCHHHHC
GLLVNVPTIGVAKKRLCGKFAPLDEATDALAPLEDKGEQLGWVWRSKARCNPLFISTGHR
CEEEECCCCHHHHHHHHHCCCCCHHHHHHHCCCHHCCCHHHHHHHCCCCCCEEEEECCCC
VGADSALAWVQRCMAGYRLPEPTRWADAIASRRPAFQRWLQQHPEVSQ
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA