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The map label for this gene is hemB [H]

Identifier: 157368504

GI number: 157368504

Start: 284189

End: 285211

Strand: Direct

Name: hemB [H]

Synonym: Spro_0255

Alternate gene names: 157368504

Gene position: 284189-285211 (Clockwise)

Preceding gene: 157368503

Following gene: 157368506

Centisome position: 5.22

GC content: 56.89

Gene sequence:

>1023_bases
ATGAGCTATGCATTTCCGGGCACCTTCCCAGGTCGCCGTATGCGCCGCGTGCGCCGTCATGATTTCAGTCGTCGCCTGGT
GGCGGAGAACCAACTGACGGTTAACGACCTGATCTACCCGGTATTTGTCATGGAAGGCAGTAACCGTCAGGAAGCGGTGG
CATCCATGCCTGGCGTATCGCGCATGAGCATCGACCTGCTGGTTAAAGAAGCGGAAGCCATCGCCAAGCTGGGCGTGCCG
GTGATCTCCCTGTTCCCGGTGATTGAGCCTGGCCTGAAGTCACTGCACGCAGAAGAAGCTTATAACCCGGACGGTCTGGT
GCAGCGTACGGTACGTGCGTTGAAAGACGCGGTGCCAGAGCTGGGGATTTTGACTGATGTGGCGCTCGATCCCTACACCA
CCCACGGTCAGGACGGCATCATTGACGAGCAAGGTTATGTTATTAACGACATCACCAAAGAGATTTTGGTGCGTCAGGCT
CTGTCCCATGCTGAAGCCGGTGCGGAAATCGTGGCGCCGAGCGACATGATGGATGGCCGTATTGGTGCGATCCGCGATCA
ACTGGAGCATCAGGGCCTGATTAACACTCAGATCATGGCTTACTCTGCCAAATATGCGTCTTGCTACTATGGCCCGTTCC
GTGATGCGCTCGGTTCCAGCGGCAATCTGAAAGGTGGCAACAAGAAAACCTACCAGATGGACCCTGCCAACAGCGACGAA
GCGCTGCAGGAAATTGCGCAGGATCTGCAGGAAGGCGCAGATATGGTCATGGTGAAGCCGGGCATGCCGTATCTGGACGT
GGTACGTCGGGTGAAAGACACCTTTGGTGTGCCGACTTTCGCCTATCAGGTGTCCGGCGAATACGCGATGCACATGGCGG
CGATTCAAAACGGTTGGTTGCAGGAACAACCGGCGGTGATGGAGTCGTTGATGTGCTTCAAGCGTGCCGGTGCCGATGGT
GTGCTGACCTACTTCGCCAAGCGGGTAGCTCAGTGGATGCATGACGACGCGATGCAGCGCTAA

Upstream 100 bases:

>100_bases
AGGTGGCCGCCTGGCGACAAGAAGATCCCGAATGGCTGGGGCAAAAAATCGATGAGAACGCCCGCCGGCTATTCCGGCTG
GAATGAGTTAGGAGAAAACT

Downstream 100 bases:

>100_bases
GGCGCGAGTATCCAATGATAAAGGCGCCTTGGGCGCCTTTTTTACATTACAGCTTTTGGAACTGTCGGTTGTCCACGCTT
TGGCTGACCTGTTTGTTGAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MSYAFPGTFPGRRMRRVRRHDFSRRLVAENQLTVNDLIYPVFVMEGSNRQEAVASMPGVSRMSIDLLVKEAEAIAKLGVP
VISLFPVIEPGLKSLHAEEAYNPDGLVQRTVRALKDAVPELGILTDVALDPYTTHGQDGIIDEQGYVINDITKEILVRQA
LSHAEAGAEIVAPSDMMDGRIGAIRDQLEHQGLINTQIMAYSAKYASCYYGPFRDALGSSGNLKGGNKKTYQMDPANSDE
ALQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWLQEQPAVMESLMCFKRAGADG
VLTYFAKRVAQWMHDDAMQR

Sequences:

>Translated_340_residues
MSYAFPGTFPGRRMRRVRRHDFSRRLVAENQLTVNDLIYPVFVMEGSNRQEAVASMPGVSRMSIDLLVKEAEAIAKLGVP
VISLFPVIEPGLKSLHAEEAYNPDGLVQRTVRALKDAVPELGILTDVALDPYTTHGQDGIIDEQGYVINDITKEILVRQA
LSHAEAGAEIVAPSDMMDGRIGAIRDQLEHQGLINTQIMAYSAKYASCYYGPFRDALGSSGNLKGGNKKTYQMDPANSDE
ALQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWLQEQPAVMESLMCFKRAGADG
VLTYFAKRVAQWMHDDAMQR
>Mature_339_residues
SYAFPGTFPGRRMRRVRRHDFSRRLVAENQLTVNDLIYPVFVMEGSNRQEAVASMPGVSRMSIDLLVKEAEAIAKLGVPV
ISLFPVIEPGLKSLHAEEAYNPDGLVQRTVRALKDAVPELGILTDVALDPYTTHGQDGIIDEQGYVINDITKEILVRQAL
SHAEAGAEIVAPSDMMDGRIGAIRDQLEHQGLINTQIMAYSAKYASCYYGPFRDALGSSGNLKGGNKKTYQMDPANSDEA
LQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWLQEQPAVMESLMCFKRAGADGV
LTYFAKRVAQWMHDDAMQR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=312, Percent_Identity=39.1025641025641, Blast_Score=234, Evalue=6e-62,
Organism=Escherichia coli, GI87081728, Length=324, Percent_Identity=43.8271604938272, Blast_Score=238, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6321398, Length=325, Percent_Identity=35.3846153846154, Blast_Score=193, Evalue=3e-50,
Organism=Drosophila melanogaster, GI21358291, Length=316, Percent_Identity=37.6582278481013, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37625; Mature: 37493

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAFPGTFPGRRMRRVRRHDFSRRLVAENQLTVNDLIYPVFVMEGSNRQEAVASMPGVS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEEECCCHHHHHHHCCCCC
RMSIDLLVKEAEAIAKLGVPVISLFPVIEPGLKSLHAEEAYNPDGLVQRTVRALKDAVPE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
LGILTDVALDPYTTHGQDGIIDEQGYVINDITKEILVRQALSHAEAGAEIVAPSDMMDGR
CCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCH
IGAIRDQLEHQGLINTQIMAYSAKYASCYYGPFRDALGSSGNLKGGNKKTYQMDPANSDE
HHHHHHHHHHCCCCHHHHHEEECHHHHHCCCHHHHHCCCCCCCCCCCCCEEEECCCCCHH
ALQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWL
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCC
QEQPAVMESLMCFKRAGADGVLTYFAKRVAQWMHDDAMQR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYAFPGTFPGRRMRRVRRHDFSRRLVAENQLTVNDLIYPVFVMEGSNRQEAVASMPGVS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEEECCCHHHHHHHCCCCC
RMSIDLLVKEAEAIAKLGVPVISLFPVIEPGLKSLHAEEAYNPDGLVQRTVRALKDAVPE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
LGILTDVALDPYTTHGQDGIIDEQGYVINDITKEILVRQALSHAEAGAEIVAPSDMMDGR
CCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCH
IGAIRDQLEHQGLINTQIMAYSAKYASCYYGPFRDALGSSGNLKGGNKKTYQMDPANSDE
HHHHHHHHHHCCCCHHHHHEEECHHHHHCCCHHHHHCCCCCCCCCCCCCEEEECCCCCHH
ALQEIAQDLQEGADMVMVKPGMPYLDVVRRVKDTFGVPTFAYQVSGEYAMHMAAIQNGWL
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCC
QEQPAVMESLMCFKRAGADGVLTYFAKRVAQWMHDDAMQR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]