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The map label for this gene is livJ [H]

Identifier: 157368477

GI number: 157368477

Start: 257074

End: 258189

Strand: Direct

Name: livJ [H]

Synonym: Spro_0228

Alternate gene names: 157368477

Gene position: 257074-258189 (Clockwise)

Preceding gene: 157368473

Following gene: 157368478

Centisome position: 4.72

GC content: 56.45

Gene sequence:

>1116_bases
ATGAAATTAACAAAAGGTAAGGCATTGTTGGCTGGCTGTATCGCGATGGCAATCGGGCATGCGGCAATGGCAAAAGACAT
TAAGGTGGCGATCGTCGGGGCGATGTCCGGCCCGGTCGCACAGTATGGCGATATGGAGTTTACCGGTGCCCGTCAGGCAA
TTGCCGATATCAATGCCAAGGGTGGCATCAAGGGCGATAAGTTGGTTGGTGTGGAATACGACGATGCCTGTGATCCGAAA
CAGGCGGTAGCGGTGGCCAACAAGGTGATCAACGACGGTATCCGTTATGTGATCGGCCACCTGTGCTCGTCCTCGACGCA
GCCGGCTTCCGATATTTATGAAGATGAAGGCGTGATCATGATTACCCCGGCGGCCACCAACGCCGATCTGACCACCCGTG
GTTACAAGATGATCATGCGTACCACCGGCCTGGACTCCGATCAGGGCCCGACCGCAGCCAAGTACATCCTCAGTGAAATC
AAACCCAAGCGCATTGCCGTGATCCACGACAAGCAGCAGTATGGCGAAGGCCTGGCACGCTCGGTACGCGACAGTCTGAA
AAAACAGGGTACCGACGTGGTGATGTTTGAAGGTATCACCGCCGGCGATAAAGATTTCTCCACTCTGGTGGCACGCCTGA
AGAAAGAGAACGTCGATTTTGTTTACTTCGGCGGCTACTACCCGGAAATGGGCCAAATCCTGCGCCAGGCCAAACAGGCC
GGGCTGACCACCCGCTTTATGGGGCCAGAGGGGGTGGGCAACTCCTCACTGTCTAACATCGCCGGTGCGGCTTCCGAAGG
CATGCTGGTGACCTTGCCGAAACGCTACGATCAGGTTCCAGCCAACAAACCTGTGGTCGATGCGCTGAAAGCCAAGAAAC
TGGATCCGACCGGGCCGTTTGTCTGGACCACCTACGCCGCACTGCAATCTCTGACCACCGGGATGGAGCGCAGTGGCAGC
CAAGAGCCGGCCGATATCGTCAAGGATTTGAAAACCGGCAAGCCGGTGGACACCGTGATGGGGCCGCTGAGCTGGGATGA
CAAGGGCGATCTGAAAGGCTTCGAGTTCGGCGTGTTTGAATGGCATGCAGACGGCACATCAACACCAATCAAATAA

Upstream 100 bases:

>100_bases
ACACCTAAATCATTATTACTTATGAATAAAAGCAGTAAATAAGGGTCAGGTGCAGGCAAACACAACAGGTACATTCACAA
CGACAGATGGGGATTTGCGG

Downstream 100 bases:

>100_bases
GCCATAATCATCAGGGTAGGACGCAGTTCTGCTGCGCCCGCGGGCCGGCTTGCCGGCTCCGTATCCCTTTACCTGAATGC
TCTTCGCATTCAGGTTTCGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKLTKGKALLAGCIAMAIGHAAMAKDIKVAIVGAMSGPVAQYGDMEFTGARQAIADINAKGGIKGDKLVGVEYDDACDPK
QAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYILSEI
KPKRIAVIHDKQQYGEGLARSVRDSLKKQGTDVVMFEGITAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
GLTTRFMGPEGVGNSSLSNIAGAASEGMLVTLPKRYDQVPANKPVVDALKAKKLDPTGPFVWTTYAALQSLTTGMERSGS
QEPADIVKDLKTGKPVDTVMGPLSWDDKGDLKGFEFGVFEWHADGTSTPIK

Sequences:

>Translated_371_residues
MKLTKGKALLAGCIAMAIGHAAMAKDIKVAIVGAMSGPVAQYGDMEFTGARQAIADINAKGGIKGDKLVGVEYDDACDPK
QAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYILSEI
KPKRIAVIHDKQQYGEGLARSVRDSLKKQGTDVVMFEGITAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
GLTTRFMGPEGVGNSSLSNIAGAASEGMLVTLPKRYDQVPANKPVVDALKAKKLDPTGPFVWTTYAALQSLTTGMERSGS
QEPADIVKDLKTGKPVDTVMGPLSWDDKGDLKGFEFGVFEWHADGTSTPIK
>Mature_371_residues
MKLTKGKALLAGCIAMAIGHAAMAKDIKVAIVGAMSGPVAQYGDMEFTGARQAIADINAKGGIKGDKLVGVEYDDACDPK
QAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYILSEI
KPKRIAVIHDKQQYGEGLARSVRDSLKKQGTDVVMFEGITAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
GLTTRFMGPEGVGNSSLSNIAGAASEGMLVTLPKRYDQVPANKPVVDALKAKKLDPTGPFVWTTYAALQSLTTGMERSGS
QEPADIVKDLKTGKPVDTVMGPLSWDDKGDLKGFEFGVFEWHADGTSTPIK

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=367, Percent_Identity=76.8392370572207, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1789867, Length=365, Percent_Identity=75.8904109589041, Blast_Score=592, Evalue=1e-171,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39612; Mature: 39612

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKGKALLAGCIAMAIGHAAMAKDIKVAIVGAMSGPVAQYGDMEFTGARQAIADINAK
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHCCCCCCCCHHHHHHHCCCC
GGIKGDKLVGVEYDDACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIM
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEE
ITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYILSEIKPKRIAVIHDKQQYGEGLAR
EECCCCCCCCCHHHHHEEHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
SVRDSLKKQGTDVVMFEGITAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
GLTTRFMGPEGVGNSSLSNIAGAASEGMLVTLPKRYDQVPANKPVVDALKAKKLDPTGPF
CCEEEECCCCCCCCCHHHHHHCCCCCCEEEECCHHHCCCCCCCCHHHHHHHCCCCCCCCC
VWTTYAALQSLTTGMERSGSQEPADIVKDLKTGKPVDTVMGPLSWDDKGDLKGFEFGVFE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEEEE
WHADGTSTPIK
EECCCCCCCCC
>Mature Secondary Structure
MKLTKGKALLAGCIAMAIGHAAMAKDIKVAIVGAMSGPVAQYGDMEFTGARQAIADINAK
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHCCCCCCCCHHHHHHHCCCC
GGIKGDKLVGVEYDDACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIM
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEE
ITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYILSEIKPKRIAVIHDKQQYGEGLAR
EECCCCCCCCCHHHHHEEHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
SVRDSLKKQGTDVVMFEGITAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
GLTTRFMGPEGVGNSSLSNIAGAASEGMLVTLPKRYDQVPANKPVVDALKAKKLDPTGPF
CCEEEECCCCCCCCCHHHHHHCCCCCCEEEECCHHHCCCCCCCCHHHHHHHCCCCCCCCC
VWTTYAALQSLTTGMERSGSQEPADIVKDLKTGKPVDTVMGPLSWDDKGDLKGFEFGVFE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEEEE
WHADGTSTPIK
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA