Click here to switch to the map view.

The map label for this gene is rpoH [H]

Identifier: 157368473

GI number: 157368473

Start: 252100

End: 252957

Strand: Direct

Name: rpoH [H]

Synonym: Spro_0224

Alternate gene names: 157368473

Gene position: 252100-252957 (Clockwise)

Preceding gene: 157368472

Following gene: 157368477

Centisome position: 4.63

GC content: 54.43

Gene sequence:

>858_bases
ATGACCAAAGAAATGCAAACTTTAGCTTTAGTGCCCCAGGGCAGTTTGGAAGCCTATATCCGGGCCGCCAACACCTATCC
GATGCTGACGGCAGAGGAAGAGCGGGAGCTGGCTGAACGGCTGCATTATCAGGGCGATCTGGAAGCCGCTAAGCAGCTGA
TCCTGTCTCACCTGCGCTTTGTCGCTCATATTGCCCGCAATTACTCTGGTTACGGCCTGCCGCAGGCGGATCTGATCCAG
GAAGGTAATATCGGCTTGATGAAAGCTGTTCGCCGCTTCAACCCAGAGGTTGGGGTGCGCCTGGTTTCCTTTGCGGTGCA
CTGGATCAAGGCAGAAATTCACGAGTACGTACTGCGCAACTGGCGTATCGTCAAAGTTGCTACCACCAAGGCACAGCGCA
AGCTGTTCTTTAACCTGCGTAAAACCAAGCAGCGTCTGGGCTGGTTCAACCAGGACGAGGTGGAATTGGTGGCCCGTGAA
CTGGGCGTGACCAGCAAGGACGTACGCGAGATGGAATCTCGCATGGCGGCACAGGATATGACTTTCGATCCAACGCCGGA
CGACGACGCCCGTGAAGGCCAGTCGATGGCACCAGTGCTGTACCTGCAGGATAAAAGCTCTGACTTTGCTGAAGGTATCG
AAGAAGATAACTGGGAAAGCAACGCCGCAGACAAACTGGCCTACGCGATGGAAGGCCTGGACGAGCGTAGTCAGCATATT
ATTCGTGCCCGTTGGCTGGACGACGATAACAAGTCCACGCTGCAAGAGCTGGCCGATCAATATGGCGTTTCCGCTGAGCG
TGTTCGCCAGTTGGAAAAAAATGCCATGAAGAAACTGAAAATGGCGATCGAAGCCTAA

Upstream 100 bases:

>100_bases
GCGTGTGGACCGCTTTTGCAATAGCAACTGCCGTAGAATCAATTATTTCCCCGCCGTGATAAATCACCTGCATGACCTGT
TTTTTCAAGAGAGGGTTTGA

Downstream 100 bases:

>100_bases
TCCCGCGTAAGCGTTAAAAAGCCCGGCTAGCCGGGCTTTTTTGTGCCTGAAATTCGGCGCTATGGCGTAACGGCAGCGAA
GTCTGGCGCCGGGCAGCTAT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTKEMQTLALVPQGSLEAYIRAANTYPMLTAEEERELAERLHYQGDLEAAKQLILSHLRFVAHIARNYSGYGLPQADLIQ
EGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVARE
LGVTSKDVREMESRMAAQDMTFDPTPDDDAREGQSMAPVLYLQDKSSDFAEGIEEDNWESNAADKLAYAMEGLDERSQHI
IRARWLDDDNKSTLQELADQYGVSAERVRQLEKNAMKKLKMAIEA

Sequences:

>Translated_285_residues
MTKEMQTLALVPQGSLEAYIRAANTYPMLTAEEERELAERLHYQGDLEAAKQLILSHLRFVAHIARNYSGYGLPQADLIQ
EGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVARE
LGVTSKDVREMESRMAAQDMTFDPTPDDDAREGQSMAPVLYLQDKSSDFAEGIEEDNWESNAADKLAYAMEGLDERSQHI
IRARWLDDDNKSTLQELADQYGVSAERVRQLEKNAMKKLKMAIEA
>Mature_284_residues
TKEMQTLALVPQGSLEAYIRAANTYPMLTAEEERELAERLHYQGDLEAAKQLILSHLRFVAHIARNYSGYGLPQADLIQE
GNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVAREL
GVTSKDVREMESRMAAQDMTFDPTPDDDAREGQSMAPVLYLQDKSSDFAEGIEEDNWESNAADKLAYAMEGLDERSQHII
RARWLDDDNKSTLQELADQYGVSAERVRQLEKNAMKKLKMAIEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=285, Percent_Identity=84.9122807017544, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1789098, Length=280, Percent_Identity=27.5, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1789448, Length=239, Percent_Identity=30.1255230125523, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32734; Mature: 32602

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEMQTLALVPQGSLEAYIRAANTYPMLTAEEERELAERLHYQGDLEAAKQLILSHLRF
CCCCCCCEEECCCCHHHHHHHHCCCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAHIARNYSGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVARELGVTSKDVREMESRMAAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
TFDPTPDDDAREGQSMAPVLYLQDKSSDFAEGIEEDNWESNAADKLAYAMEGLDERSQHI
CCCCCCCCHHHCCCCCCCEEEEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IRARWLDDDNKSTLQELADQYGVSAERVRQLEKNAMKKLKMAIEA
HHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKEMQTLALVPQGSLEAYIRAANTYPMLTAEEERELAERLHYQGDLEAAKQLILSHLRF
CCCCCCEEECCCCHHHHHHHHCCCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAHIARNYSGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVELVARELGVTSKDVREMESRMAAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
TFDPTPDDDAREGQSMAPVLYLQDKSSDFAEGIEEDNWESNAADKLAYAMEGLDERSQHI
CCCCCCCCHHHCCCCCCCEEEEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IRARWLDDDNKSTLQELADQYGVSAERVRQLEKNAMKKLKMAIEA
HHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]