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The map label for this gene is nac [H]

Identifier: 157158368

GI number: 157158368

Start: 2238843

End: 2239760

Strand: Reverse

Name: nac [H]

Synonym: EcE24377A_2270

Alternate gene names: 157158368

Gene position: 2239760-2238843 (Counterclockwise)

Preceding gene: 157155230

Following gene: 157158758

Centisome position: 44.98

GC content: 48.15

Gene sequence:

>918_bases
ATGAACTTCAGACGCCTGAAATACTTCGTAAAAATTGTAGATATTGGTAGCCTGACCCAGGCTGCTGAAGTATTGCATAT
CGCACAACCAGCGCTCAGCCAGCAGGTTGCCACACTGGAAGGTGAGTTAAATCAACAACTTTTGATCCGTACAAAGCGGG
GCGTTACGCCAACAGACGCCGGAAAAATTCTCTATACCCATGCGCGGGCCATTTTACGTCAGTGTGAACAGGCCCAACTG
GCGGTGCATAACGTTGGTCAGGCATTATCGGGGCAAGTCTCGATTGGCTTTGCACCAGGAACCGCTGCGTCATCCATCAC
CATGCCCTTATTACAGGCGGTTCGCGCTGAATTTCCGGAGATCGTTATCTATCTTCATGAAAATAGTGGTGCAGTGCTTA
ACGAAAAATTGATAAATCACCAACTCGATATGGCGGTGATTTATGAGCATTCCCCTGTGGCTGGTGTATCCAGTCAGGCT
TTGCTGAAAGAAGATCTTTTTCTGGTAGGTACTCAAGATTGCCCGGGGCAAAGCGTTGATGTGAATGCTATTGCGAAAAT
GAACCTCTTTCTCCCCAGTGATTACAGTGCTATTAGACTTCGTGTTGATGAGGCTTTTTCCCTACGGCGACTCACGGCAA
AAGTTATTGGTGAAATTGAGTCTATTGCCACGCTTACCGCAGCGATTGCCAGCGGCATGGGCGTTGCAGTATTACCCGAA
TCGGCCGCGCGTTCGTTATGTGGCGCAGTAAATGGGTGGATGTCACGCATTACCACTCCTTCCATGAGTCTCTCTTTGTC
ATTAAATTTACCCGCCAGAGCGAACTTATCGCCACAGGCACAGGCAGTGAAAGAGTTGTTAATGTCAGTGATTAGTTCTC
CAGTGATGGAAAAAAGGCAGTGGCAATTGGTGAGCTAA

Upstream 100 bases:

>100_bases
TAGCTTGTACATCAACGCCAAAATAAAACTGGCAAGCATCTTGCAATCTGGTTGTAAGTAATGGCGGCACTTGGGCCGAT
TCTTAAAAACCGGAGGCAAC

Downstream 100 bases:

>100_bases
GTGTTATATCGGAATGGAATAAGATGCGGGTTTTTATTATTTGTTATGCCGGGCATTAGACTTTAACAATAACGGGAAAT
CTGAACTGCCCGGAGTTTAC

Product: nitrogen assimilation transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS

Sequences:

>Translated_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS
>Mature_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=305, Percent_Identity=99.672131147541, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=30.6122448979592, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=28.2786885245902, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=27.8884462151394, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790208, Length=245, Percent_Identity=27.7551020408163, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788887, Length=175, Percent_Identity=33.1428571428571, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787601, Length=257, Percent_Identity=25.2918287937743, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI145693105, Length=238, Percent_Identity=23.109243697479, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789204, Length=278, Percent_Identity=24.4604316546763, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367136, Length=254, Percent_Identity=25.9842519685039, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786202, Length=248, Percent_Identity=24.5967741935484, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=258, Percent_Identity=24.4186046511628, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787589, Length=253, Percent_Identity=28.0632411067194, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=30.0813008130081, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32836; Mature: 32836

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDA
CCHHHEEHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCC
GKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCE
IVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQSVD
EEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCCC
VNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
HHEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
WQLVS
CCCCC
>Mature Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELNQQLLIRTKRGVTPTDA
CCHHHEEHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCC
GKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCE
IVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQSVD
EEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCCC
VNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
HHEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
WQLVS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]