Click here to switch to the map view.

The map label for this gene is cobS

Identifier: 157157942

GI number: 157157942

Start: 2242306

End: 2243049

Strand: Reverse

Name: cobS

Synonym: EcE24377A_2274

Alternate gene names: 157157942

Gene position: 2243049-2242306 (Counterclockwise)

Preceding gene: 157156453

Following gene: 157157289

Centisome position: 45.04

GC content: 53.9

Gene sequence:

>744_bases
ATGAGTAAATTATTTTGGGCGATGCTCTCTTTTATTACGCGTCTGCCTGTACCGCGTCGCTGGTCCCAGGGACTGGATTT
CGAGCATTATTCTCGCGGTATTATTACTTTTCCTTTAATTGGATTATTGCTTGGCGCGATTAGCGGGCTGGTCTTCATGG
TGCTGCAGGCATGGTGTGGCGTACCACTGGCGGCACTGTTTAGCGTGCTTGTGCTGGCGCTGATGACCGGGGGATTCCAC
CTGGACGGCCTCGCCGATACCTGTGATGGCGTATTTTCTGCACGTAGCCGCGATCGCATGCTGGAAATCATGCGTGATAG
CCGTTTAGGCACCCACGGCGGTCTGGCATTGATTTTTGTGGTACTGGCAAAGATTCTGGTGTTAAGCGAGTTGGCTCTGC
GTGGCGAACCGATCCTTGCATCGCTGGCGGCAGCATGTGCGGTCAGCCGCGGGACTGCCGCATTATTGATGTATCGTCAT
CGTTATGCCCGGGAAGAAGGGCTTGGCAATGTGTTCATTGGCAAAATTGATGGGCGACAAACCTGCGTCACACTCGGCTT
AGCCGCTATTTTTGCTGCCGTATTATTGCCGGGTATGCATGGTGTGGCTGCTATGGTGGTGACGATGGTGGCGATTTTCA
TCCTCGGTCAGCTTCTCAAACGTACGCTGGGCGGGCAAACCGGCGATACGCTGGGAGCTGCTATAGAGCTTGGTGAACTG
GTCTTCCTGCTGGCACTGCTATAA

Upstream 100 bases:

>100_bases
CACGACATTTTCGTGATATTGCCGGGCGGGTAAATCAGCAGCTGGCCGCTGCGGCAAATGAAGTATGGTTGGTGGTTTCG
GGTATTGGAGTAAAAATCAA

Downstream 100 bases:

>100_bases
AGAGACGTGACATGCAAACACTTGCCGATTTACTGAATACGATCCCTGCCATCGATCCTGCCGCGATGTCGCGTGCACAA
CGGCATATTGACGGGTTACT

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLLGAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFH
LDGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGEL
VFLLALL

Sequences:

>Translated_247_residues
MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLLGAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFH
LDGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGEL
VFLLALL
>Mature_246_residues
SKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLLGAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHL
DGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRHR
YAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGELV
FLLALL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=98.7854251012146, Blast_Score=475, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_ECO24 (A7ZNF6)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463335.1
- STRING:   A7ZNF6
- EnsemblBacteria:   EBESCT00000021994
- GeneID:   5586672
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2274
- eggNOG:   COG0368
- GeneTree:   EBGT00050000010502
- HOGENOM:   HBG729392
- OMA:   HDSRIGA
- ProtClustDB:   PRK00235
- BioCyc:   ECOL331111:ECE24377A_2274-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26396; Mature: 26265

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e96c3d8)-; HASH(0x1ec3b834)-; HASH(0x1bf7cad4)-; HASH(0x1ebb7164)-; HASH(0x1e68c120)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLLGAISGLVFMVLQAWCG
CHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFV
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDGRQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCHH
TCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGEL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VFLLALL
HHHHHHH
>Mature Secondary Structure 
SKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLLGAISGLVFMVLQAWCG
HHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFV
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDGRQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCHH
TCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGEL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VFLLALL
HHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA