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The map label for this gene is sppA [H]

Identifier: 157157447

GI number: 157157447

Start: 2187783

End: 2189093

Strand: Reverse

Name: sppA [H]

Synonym: EcE24377A_2225

Alternate gene names: 157157447

Gene position: 2189093-2187783 (Counterclockwise)

Preceding gene: 157158336

Following gene: 157158086

Centisome position: 43.96

GC content: 58.96

Gene sequence:

>1311_bases
ATGCCGTGGAACAATTTTCCGCACCTCGCCGCTAGGGCGTTCAATCAACCGCTTTTGCTGGAGCCCGCCTACGCGCGGGT
ATTTTTTTCTGCGCTGAGCGACCGGTTCGGTACCGGGCGACTGATTGATACAGCGTCAGGAGAGGTAATGAACAGCGACG
AAATGAACGCGCTCGCGATGGGGTGGGACAGCAGCGAGCGAACACGCCAGAAATCGTATCGCGTGGAGCGTGGTATAGCC
GTTCTGCCGGTTACCGGGACGCTGGTTCATAAATTGGGTTATATCAATCCGGTCAGCGGGATGAGTGGTTACGACGGAAT
CGCAAAACGCCTGCAGCAGGCGATTTCTGATCCCGATGTTAAGGGGATCCTGCTGGATATTGATTCCCCTGGCGGTGAGG
TCGCCGGCGCGTTTGATACCGCTGATTTAATCGCCCGGGCGCGAGAGCAAAAACCGGTGTGGGCGCTGGCCAGCGATACG
GCCTGCAGCGCCGCATATTTGCTGGCGTCAGCGTGTTCGCGCCGGCTGATAACGCAGACCGGCACGGTTGGTTCAATCGG
TGTCCTGATGGCTCACCGCTGCGTCGAAAAGGCGCTGGAGATTGCCGGCGTTGACGTGACGCTGATTTACGCCGGCGCGC
ACAAAGTCGACGGGAACCCGTATTCCCAGCTGCCCGACGACGTTCGCGACGAATTCCAGCTGAGTATTAACAGCACACGC
GAGCAGTTCGCGCAAAAAGTCTCGGATTATACCGGGCTGAAAAAATCCAGGGTGCTGGCCACAGAGGCCGCAGTATTTAT
CGGCGCGGACGCGATTAAATCTGGTCTCGCTGATCAACTCGTTAATTACGCGGACGCTATCGCAGTGATGGCCGACGCAC
TGAAACCAAAAACGGAGCGATTTATGCCAGGTACAACAGAAACCACGGCGGAGACCACGACCACAGAACAAACCGCGGCT
ACGACTACGGTCGCGCCGGTTGAGTCCAACGCGGAGCAGATTCGCGCGGACGCCGCATCGAGCGAACTGGCACGCGTGAT
GGCCATCATCAACTGTCCCGAAGCTGTTGGGCGCGAGGCGCAGGCAAAAGCGCTCGCTGCCGTCCCCGGGATGACGGTCG
GGCAGGCGCAGGCTGTCCTCGCGGCAGCACCGCAAACAGCGCAGGCGCGGACAGAAACGGCGCTCGATACACTCATGAGC
ACTGAATCACCGGAAACTATTCAGGATGCCGGCAGCACCACGGCAACAGGAACAACCGCAAACGTCTCGATGCTGGTCGC
GGCAGGGCGTTCAATTTTAGGGGATGAATAA

Upstream 100 bases:

>100_bases
AATACGAAATTCAGCGCCGGCGCGAAATGGGGCTGAGCGAACCATCCTGGTCAGTATCTCACCCCTCAAATACCAACGAT
AACGGCTGGGGAGGTAACTG

Downstream 100 bases:

>100_bases
TGACCACAGAAACGTATAGCCCGGATGATTTTATTCTGGGACCCGATCTTGTTGTGACGACCGTCGGTCATTTTCAGGGC
GGGATCAACGTACCCCGGCT

Product: S49 family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPWNNFPHLAARAFNQPLLLEPAYARVFFSALSDRFGTGRLIDTASGEVMNSDEMNALAMGWDSSERTRQKSYRVERGIA
VLPVTGTLVHKLGYINPVSGMSGYDGIAKRLQQAISDPDVKGILLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDT
ACSAAYLLASACSRRLITQTGTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDGNPYSQLPDDVRDEFQLSINSTR
EQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGLADQLVNYADAIAVMADALKPKTERFMPGTTETTAETTTTEQTAA
TTTVAPVESNAEQIRADAASSELARVMAIINCPEAVGREAQAKALAAVPGMTVGQAQAVLAAAPQTAQARTETALDTLMS
TESPETIQDAGSTTATGTTANVSMLVAAGRSILGDE

Sequences:

>Translated_436_residues
MPWNNFPHLAARAFNQPLLLEPAYARVFFSALSDRFGTGRLIDTASGEVMNSDEMNALAMGWDSSERTRQKSYRVERGIA
VLPVTGTLVHKLGYINPVSGMSGYDGIAKRLQQAISDPDVKGILLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDT
ACSAAYLLASACSRRLITQTGTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDGNPYSQLPDDVRDEFQLSINSTR
EQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGLADQLVNYADAIAVMADALKPKTERFMPGTTETTAETTTTEQTAA
TTTVAPVESNAEQIRADAASSELARVMAIINCPEAVGREAQAKALAAVPGMTVGQAQAVLAAAPQTAQARTETALDTLMS
TESPETIQDAGSTTATGTTANVSMLVAAGRSILGDE
>Mature_435_residues
PWNNFPHLAARAFNQPLLLEPAYARVFFSALSDRFGTGRLIDTASGEVMNSDEMNALAMGWDSSERTRQKSYRVERGIAV
LPVTGTLVHKLGYINPVSGMSGYDGIAKRLQQAISDPDVKGILLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDTA
CSAAYLLASACSRRLITQTGTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDGNPYSQLPDDVRDEFQLSINSTRE
QFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGLADQLVNYADAIAVMADALKPKTERFMPGTTETTAETTTTEQTAAT
TTVAPVESNAEQIRADAASSELARVMAIINCPEAVGREAQAKALAAVPGMTVGQAQAVLAAAPQTAQARTETALDTLMST
ESPETIQDAGSTTATGTTANVSMLVAAGRSILGDE

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.- [C]

Molecular weight: Translated: 45921; Mature: 45790

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWNNFPHLAARAFNQPLLLEPAYARVFFSALSDRFGTGRLIDTASGEVMNSDEMNALAM
CCCCCCCHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCEEECCCCCEECCCCCCEEEE
GWDSSERTRQKSYRVERGIAVLPVTGTLVHKLGYINPVSGMSGYDGIAKRLQQAISDPDV
CCCCHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCC
KGILLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDTACSAAYLLASACSRRLITQT
CEEEEEECCCCCCEECCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
GTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDGNPYSQLPDDVRDEFQLSINSTR
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCEECCCCCHHHCCHHHHHHHEEHHHHHH
EQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGLADQLVNYADAIAVMADALKPKTER
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FMPGTTETTAETTTTEQTAATTTVAPVESNAEQIRADAASSELARVMAIINCPEAVGREA
CCCCCCCCCCCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCHH
QAKALAAVPGMTVGQAQAVLAAAPQTAQARTETALDTLMSTESPETIQDAGSTTATGTTA
HHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
NVSMLVAAGRSILGDE
HHHHHHHHCCHHCCCC
>Mature Secondary Structure 
PWNNFPHLAARAFNQPLLLEPAYARVFFSALSDRFGTGRLIDTASGEVMNSDEMNALAM
CCCCCCHHHHHHCCCCEEECHHHHHHHHHHHHHCCCCCCEEECCCCCEECCCCCCEEEE
GWDSSERTRQKSYRVERGIAVLPVTGTLVHKLGYINPVSGMSGYDGIAKRLQQAISDPDV
CCCCHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCC
KGILLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDTACSAAYLLASACSRRLITQT
CEEEEEECCCCCCEECCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
GTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDGNPYSQLPDDVRDEFQLSINSTR
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCEECCCCCHHHCCHHHHHHHEEHHHHHH
EQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGLADQLVNYADAIAVMADALKPKTER
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
FMPGTTETTAETTTTEQTAATTTVAPVESNAEQIRADAASSELARVMAIINCPEAVGREA
CCCCCCCCCCCCCCCHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCHH
QAKALAAVPGMTVGQAQAVLAAAPQTAQARTETALDTLMSTESPETIQDAGSTTATGTTA
HHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCC
NVSMLVAAGRSILGDE
HHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]