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The map label for this gene is istB [H]

Identifier: 157158336

GI number: 157158336

Start: 2190088

End: 2190882

Strand: Reverse

Name: istB [H]

Synonym: EcE24377A_2227

Alternate gene names: 157158336

Gene position: 2190882-2190088 (Counterclockwise)

Preceding gene: 157157074

Following gene: 157157447

Centisome position: 44.0

GC content: 54.97

Gene sequence:

>795_bases
ATGCATGAACTGGAGACACTGCTGAGTCGTCTTAAAATGGAACATCTGGGCTACCACGTTGAAAGCCTGCTGGAGCAGGC
GGCCAAAAAAGAACTGAACTACCGCGAGTTCCTGTGCATGGCGTTGCAGCAGGAATGGAACGGGAGGCATCAGCGCGGTA
TGGAGTCCCGGCTGAAACAGGCCCGCTTCCCGTGGGTCAAAACGCTGGAGCAGTTCGACTTCGGCTTCCAGCCAGGTATC
GATCGTAAGGTCGTCCGTGAGCTGGCCGGGCTGGCGTTCGTGGAGCGTAGCGAGAACGTGATCCTGCTGGGTCCGCCCGG
TGTGGGTAAAACGCACCTGGCAGTCGCTCTCGGGGTAAAAGCGGCGGACGCTGGTCATCGGGTACTGTTCATGCCACTGG
ATAAGCTGATCGCCACACTCATGAAGGCAAAACAGGAAAACCGGCTGGAAAAACAGCTACAGCAGCTTGGTTATGCCCGG
GTACTGATACTGGATGAGATAGGCTATCTGCCGATGACCCGCGAGGAAGCCAGTCTGTTCTTCCGGTTGCTGAACCGTCG
ATATGAAAAAGCGAGCATCGTGCTGACATCAAATAAAGGGTTCGCCGACTGGGGAGAGATGTTCGGCGATAACGTGCTGG
CAACGGCAATCCTGGATCGACTGCTCCACCACTCAACCACACTGAACATCAAAGGAGAAAGCTACCGGTTAAAGGAAAAA
CGCAAAGCGGGAGTGCTGGCAAAAAACGCCACGCCAATCAGTGATGATGAAATGGCGGAAAGCGGACAACATTAA

Upstream 100 bases:

>100_bases
CATCCAGCTGGCAAACCGTGCCGGAGCATCACGCCCCACTCTGGCAGCAGGTCAGTCAGGTGGAACACAGACCGTTGAGT
ACGTATGAGGAGCTGTTGTG

Downstream 100 bases:

>100_bases
TGACCAATAGCGGACATTAAAAATGGCGAAAAACGGCCAATAATCTTGGCGTTGACAGAGTTGGTAAATGCCGGCGCATA
CCAGCAAAACTCAGCAGCGG

Product: IS21 family transposition helper protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARFPWVKTLEQFDFGFQPGI
DRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVKAADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYAR
VLILDEIGYLPMTREEASLFFRLLNRRYEKASIVLTSNKGFADWGEMFGDNVLATAILDRLLHHSTTLNIKGESYRLKEK
RKAGVLAKNATPISDDEMAESGQH

Sequences:

>Translated_264_residues
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARFPWVKTLEQFDFGFQPGI
DRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVKAADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYAR
VLILDEIGYLPMTREEASLFFRLLNRRYEKASIVLTSNKGFADWGEMFGDNVLATAILDRLLHHSTTLNIKGESYRLKEK
RKAGVLAKNATPISDDEMAESGQH
>Mature_264_residues
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARFPWVKTLEQFDFGFQPGI
DRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVKAADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYAR
VLILDEIGYLPMTREEASLFFRLLNRRYEKASIVLTSNKGFADWGEMFGDNVLATAILDRLLHHSTTLNIKGESYRLKEK
RKAGVLAKNATPISDDEMAESGQH

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 30097; Mature: 30097

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
ARFPWVKTLEQFDFGFQPGIDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVK
HCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCE
AADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYARVLILDEIGYLPMTREEASLF
ECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHCCCCCCCHHHHHHH
FRLLNRRYEKASIVLTSNKGFADWGEMFGDNVLATAILDRLLHHSTTLNIKGESYRLKEK
HHHHHHHHCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
RKAGVLAKNATPISDDEMAESGQH
HHCCCEECCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MHELETLLSRLKMEHLGYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
ARFPWVKTLEQFDFGFQPGIDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAVALGVK
HCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCE
AADAGHRVLFMPLDKLIATLMKAKQENRLEKQLQQLGYARVLILDEIGYLPMTREEASLF
ECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHCCCCCCCHHHHHHH
FRLLNRRYEKASIVLTSNKGFADWGEMFGDNVLATAILDRLLHHSTTLNIKGESYRLKEK
HHHHHHHHCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
RKAGVLAKNATPISDDEMAESGQH
HHCCCEECCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]