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The map label for this gene is dusC [H]

Identifier: 157157351

GI number: 157157351

Start: 2411860

End: 2412807

Strand: Reverse

Name: dusC [H]

Synonym: EcE24377A_2429

Alternate gene names: 157157351

Gene position: 2412807-2411860 (Counterclockwise)

Preceding gene: 157156465

Following gene: 157159177

Centisome position: 48.45

GC content: 54.11

Gene sequence:

>948_bases
ATGCGTGTGTTACTGGCGCCGATGGAGGGTGTGCTTGACTCTCTGGTGCGTGAATTGCTGACCGAAGTTAACGACTACGA
TCTGTGCATCACCGAGTTTGTCCGCGTGGTGGATCAACTGCTGCCGGTAAAAGTCTTTCATCGCATTTGCCCTGAGCTAC
AAAACGCCAGCCGGACACCATCCGGTACGCTGGTGCGCGTTCAGTTGTTAGGTCAGTTCCCACAATGGCTGGCAGAGAAC
GCCGCCCGTGCGGTGGAATTAGGTTCCTGGGGCGTGGATCTCAATTGCGGCTGCCCGTCGAAAACGGTTAACGGCAGCGG
CGGCGGGGCGACGTTACTCAAAGATCCTGAACTCATCTACCAGGGTGCAAAAGCGACGCGTGAAGCTGTACCAGCGCATT
TGCCCGTCAGCGTAAAAGTGCGTCTGGGCTGGGACAGCGGTGAGAAGAAATTTGAAATCGCCGATGCGGTTCAACAGGCT
GGCGCTACGGAGCTGGTGGTGCATGGGCGGACAAAAGAGCAGGGTTACCGCGCGGAGCATATTGACTGGCAGGCGATTGG
CGAGATTCGCCAGCGGCTGAATATTCCGGTGATTGCCAACGGTGAAATCTGGGACTGGCAGAGTGCGCAACAATGCATGG
CGATCAGCGGCTGCGACGCAGTGATGATTGGTCGCGGGGCGCTCAATATTCCCAACCTGAGCCGGGTAGTAAAATATAAC
GAACCGCGAATGCCGTGGCCGGAGGTGGTTGCTTTGCTGCAAAAATATACCCGTCTGGAAAAGCAGGGCGATACCGGGTT
ATATCACGTTGCGCGGATTAAACAGTGGTTGAGTTATTTGCGTAAAGAATACGATGAAGCAACGGGATTATTTCAGCATG
TTCGGGTGTTGAATAATTCCCCTGATATTGCAAGGGCTATTCAGGCAATTGATATCGAGAAACTCTAA

Upstream 100 bases:

>100_bases
TACGGCTGGAAAGAAGATAACTGTCTGCAACCACGCTGAGTACTTTAATCCGCTGACCGGAGGCGCTAACATAGCGCCTC
ATTTTTTTGCGGGTGATGAT

Downstream 100 bases:

>100_bases
AAAACCATAGTGATGTTTTTTGACAATGTGTTAGCTCTTTATCAATAGGCCGCACGGTTGTTTGGTGGCGTCAAAATTGC
CGAGGCGTTCCCTGAAGGCC

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKATREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL

Sequences:

>Translated_315_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKATREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL
>Mature_315_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKATREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=31.1688311688312, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI8923374, Length=278, Percent_Identity=29.4964028776978, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI239788483, Length=225, Percent_Identity=31.5555555555556, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=28.8702928870293, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI239788462, Length=225, Percent_Identity=31.5555555555556, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788462, Length=315, Percent_Identity=99.0476190476191, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1789660, Length=324, Percent_Identity=28.7037037037037, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI145693211, Length=251, Percent_Identity=25.4980079681275, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25144369, Length=249, Percent_Identity=33.7349397590361, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=35.0649350649351, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=218, Percent_Identity=27.0642201834862, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=201, Percent_Identity=31.3432835820896, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585320, Length=217, Percent_Identity=30.8755760368664, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24580595, Length=179, Percent_Identity=29.608938547486, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920448, Length=179, Percent_Identity=29.608938547486, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35112; Mature: 35112

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTP
CEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIY
CCCEEEHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHH
QGAKATREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEH
HCHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
IDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATGLFQHVRVLNNS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIARAIQAIDIEKL
CHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTP
CEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIY
CCCEEEHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHH
QGAKATREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEH
HCHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
IDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATGLFQHVRVLNNS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIARAIQAIDIEKL
CHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]