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The map label for this gene is maiA [H]

Identifier: 157156465

GI number: 157156465

Start: 2414076

End: 2414720

Strand: Reverse

Name: maiA [H]

Synonym: EcE24377A_2431

Alternate gene names: 157156465

Gene position: 2414720-2414076 (Counterclockwise)

Preceding gene: 157155625

Following gene: 157157351

Centisome position: 48.49

GC content: 55.66

Gene sequence:

>645_bases
ATGAAGCTGTACAGTTTTTTTAATAGTTCAGCATCGTACCGGGTACGCATTGCGCTGGCGCTGAAAGGCATTGATTACCA
GACCGAAGGGGTGAATATCCGCATTGGTCAGCAAAATGAACTGGCCTACCGGCGGATGAATCCGGTCGGGCTGGTGCCGA
CGCTGCTGACTGACGAGGGCGAATCACTTGGACAATCGCTGGCGATTATTGACTGGATGGATCGTCACTACCCCCAAGTT
CCGCTGGTGCCGCAGGAAGAGCCGGCCCGCAACAAGGTGCTGGAAATTGTCTATGCCATCGCCTGTGATATTCATCCACT
GAATAACCTGCGCGTGCTGCGCTACCTGACCGAAGAGCTGAACGTCAGTGAAGAAGATAAAAAACGCTGGTATGCGCACT
GGATCCAGCAAGGGTTAAGCGCGGTGGAACAGCTGTTGCGCCAGAGCCAGTCGGGGCAATTTTGCGTGGGCGAGACGCCG
ACGCTGGCAGATTGCTGCCTTGTCCCGCAGTGGGCGAACGCGCTACGCATGAACTGCGATCTGAGTGGGTATCCGCGCTG
CAAAGCGGTGTACGACGCCTGCACGCAGCTGCCAGCGTTCATTGCCGCAGCGCCAGAAAATCAACAAGATAAAATTTCAG
CCTGA

Upstream 100 bases:

>100_bases
GTACGCCGGAAGGGGTTGGCCCGGTAGTGAAAGGTGATGTGATCACCGGCAACGTCGAGGGCTTAACGCCTATCGCGGTG
AAAATTGTCTGAGGTGCATG

Downstream 100 bases:

>100_bases
CCAGGAGAACCATAATGACTAAAGTGACACGCGCAGTAATTGTGGGAGGCGGGATCGGCGGTGCGGCAACTGCGTTGTCA
CTGGCTCGCCTGGGGATCAA

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEGESLGQSLAIIDWMDRHYPQV
PLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA

Sequences:

>Translated_214_residues
MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEGESLGQSLAIIDWMDRHYPQV
PLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA
>Mature_214_residues
MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEGESLGQSLAIIDWMDRHYPQV
PLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=210, Percent_Identity=43.8095238095238, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI194440732, Length=210, Percent_Identity=36.1904761904762, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI22202622, Length=159, Percent_Identity=40.8805031446541, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17551302, Length=209, Percent_Identity=39.2344497607655, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17556142, Length=209, Percent_Identity=37.799043062201, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17510461, Length=209, Percent_Identity=35.4066985645933, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24645375, Length=210, Percent_Identity=44.2857142857143, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI21355859, Length=210, Percent_Identity=44.2857142857143, Blast_Score=156, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45553325, Length=210, Percent_Identity=44.2857142857143, Blast_Score=156, Evalue=8e-39,
Organism=Drosophila melanogaster, GI21355857, Length=211, Percent_Identity=38.8625592417062, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24205; Mature: 24205

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEG
CCCCEECCCCCCEEEEEEEEEECCCEEECCEEEEECCCCCHHHHHCCCCCCCHHHCCCCC
ESLGQSLAIIDWMDRHYPQVPLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEEL
HHHCCEEHEEEHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
NVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETPTLADCCLVPQWANALRMNCD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCHHHHHHEEECCC
LSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA
CCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCC
>Mature Secondary Structure
MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEG
CCCCEECCCCCCEEEEEEEEEECCCEEECCEEEEECCCCCHHHHHCCCCCCCHHHCCCCC
ESLGQSLAIIDWMDRHYPQVPLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEEL
HHHCCEEHEEEHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
NVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETPTLADCCLVPQWANALRMNCD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCHHHHHHEEECCC
LSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA
CCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]