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The map label for this gene is yeeY

Identifier: 157156076

GI number: 157156076

Start: 2269992

End: 2270921

Strand: Reverse

Name: yeeY

Synonym: EcE24377A_2306

Alternate gene names: 157156076

Gene position: 2270921-2269992 (Counterclockwise)

Preceding gene: 157155443

Following gene: 157156766

Centisome position: 45.6

GC content: 50.65

Gene sequence:

>930_bases
ATGAAACCACTGCTGGATGTGTTGATGATCCTCGATGCCTTAGAAAAAGAAGGCAGTTTTGCGGCGGCGTCGGCCAAACT
CTATAAGACGCCGTCCGCTCTGAGTTACACCGTTCACAAGCTGGAAAGCGACCTTAATATTCAATTGCTTGATCGTAGCG
GTCACCGCGCTAAATTCACCCGTACCGGAAAAATGTTATTAGAGAAAGGGCGGGAAGTTCTGCATACCGTGCGAGAACTG
GAAAAGCAGGCGATCAAACTGCATGAAGGCTGGGAAAACGAGCTGGTGATTGGCGTGGACGACACGTTTCCTTTTTCTCT
GCTTGCGCCACTGATTGAAGCTTTTTATCAACATCATAGCGTGACGCGCCTGAAGTTTATCAATGGCGTACTCGGTGGTT
CCTGGGATGCCCTGACTCAGGGGCGAGCGGATATTATTGTTGGCGCGATGCATGAGCCACCATCTTCCAGTGAGTTTGGT
TTTTCACGGTTGGGCGATCTGGAACAAGTCTTTGCGGTCGCGCCTCATCATCCGTTAGCCCTGGAAGAAGAGCCGTTAAA
TCGTCGTATTATTAAGCGATATCGGGCGATTGTGGTGGGGGATACTGCGCAGGCAGGCGCTTCTACGGCCTCACAACTTC
TCGACGAACAAGAAGCCATTACCGTTTTCGATTTTAAAACCAAGCTGGAGCTGCAAATTAGCGGCCTCGGCTGCGGCTAT
TTACCCCGTTATCTGGCGCAACGGTTTCTCGATAGTGGCGCGTTAATCGAGAAGAAAGTGGTCGCCCAAACTCTCTTTGA
ACCCGTCTGGATTGGCTGGAATGAACAGACCGCAGGGCTTGCCAGCGGCTGGTGGCGGGATGAAATTTTAGCAAATAGTG
CGATCGCCGGTGTTTATGCAAAATCTGATGACGGAAAATCAGCCATTTAA

Upstream 100 bases:

>100_bases
TGAACTGGGATTTGAATACCAGTATCCCGATCCGCTGGTAATGCCGCTGGAGTAAACGATCACGCCAGCAGGACGCCGCA
TGCACTGCAAGGGGGCGTGT

Downstream 100 bases:

>100_bases
AGAAAAATTATTATGACAAGCCTCTCATTCTCTTGTCATTTCCCCCCCATTTAGGCACAATGCGCCGCTGTCAAAAAATG
ACTAAAAACCGACGTTTCAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESDLNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVREL
EKQAIKLHEGWENELVIGVDDTFPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQEAITVFDFKTKLELQISGLGCGY
LPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQTAGLASGWWRDEILANSAIAGVYAKSDDGKSAI

Sequences:

>Translated_309_residues
MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESDLNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVREL
EKQAIKLHEGWENELVIGVDDTFPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQEAITVFDFKTKLELQISGLGCGY
LPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQTAGLASGWWRDEILANSAIAGVYAKSDDGKSAI
>Mature_309_residues
MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESDLNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVREL
EKQAIKLHEGWENELVIGVDDTFPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQEAITVFDFKTKLELQISGLGCGY
LPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQTAGLASGWWRDEILANSAIAGVYAKSDDGKSAI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI87082024, Length=309, Percent_Identity=100, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1789492, Length=286, Percent_Identity=38.4615384615385, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1787949, Length=249, Percent_Identity=31.3253012048193, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786508, Length=293, Percent_Identity=25.938566552901, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786713, Length=277, Percent_Identity=24.9097472924188, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790262, Length=274, Percent_Identity=28.4671532846715, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787601, Length=165, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082132, Length=271, Percent_Identity=25.830258302583, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YEEY_ECOLI (P76369)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F64966
- RefSeq:   AP_002615.1
- RefSeq:   NP_416519.4
- ProteinModelPortal:   P76369
- STRING:   P76369
- EnsemblBacteria:   EBESCT00000001631
- EnsemblBacteria:   EBESCT00000014877
- GeneID:   946532
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5834
- KEGG:   eco:b2015
- EchoBASE:   EB3173
- EcoGene:   EG13392
- eggNOG:   COG0583
- GeneTree:   EBGT00050000008332
- HOGENOM:   HBG685425
- OMA:   FVFALAP
- ProtClustDB:   CLSK880269
- BioCyc:   EcoCyc:G7088-MONOMER
- Genevestigator:   P76369
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34169; Mature: 34169

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESDLNIQLLDRSGHRAKFT
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCEEEHH
RTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDTFPFSLLAPLIEAFYQHHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
VTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFGFSRLGDLEQVFAVAPHHPLA
HHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHCCCCCCC
LEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQEAITVFDFKTKLELQISGLGCGY
CCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCCCCH
LPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQTAGLASGWWRDEILANSAIAGVYA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHCCHHHEEEE
KSDDGKSAI
ECCCCCCCC
>Mature Secondary Structure
MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESDLNIQLLDRSGHRAKFT
CCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCCCEEEHH
RTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDTFPFSLLAPLIEAFYQHHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
VTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFGFSRLGDLEQVFAVAPHHPLA
HHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHCCCCCCC
LEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQEAITVFDFKTKLELQISGLGCGY
CCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCCCCH
LPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQTAGLASGWWRDEILANSAIAGVYA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHCCHHHEEEE
KSDDGKSAI
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503