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The map label for this gene is yeeF

Identifier: 157156766

GI number: 157156766

Start: 2268367

End: 2269725

Strand: Reverse

Name: yeeF

Synonym: EcE24377A_2305

Alternate gene names: 157156766

Gene position: 2269725-2268367 (Counterclockwise)

Preceding gene: 157156076

Following gene: 157159262

Centisome position: 45.58

GC content: 52.69

Gene sequence:

>1359_bases
ATGTCGCATAACGTTACTCCAAACACCTCTCGCGTGGAATTGCGTAAAACGCTTACGTTAGTTCCGGTTGTAATGATGGG
TCTTGCCTATATGCAGCCGATGACGCTGTTTGATACTTTTGGTATCGTTTCAGGCCTCACGGATGGTCATGTGCCGACAG
CCTATGCGTTCGCATTGATTGCGATCCTGTTTACGGCTCTGAGCTACGGGAAGCTGGTTCGCCGCTATCCTTCTGCTGGC
TCTGCATACACTTACGCCCAGAAATCCATTAGCCCGACTGTCGGCTTTATGGTGGGTTGGTCTTCTCTGCTCGACTATCT
GTTCGCGCCGATGATCAACATTCTGCTGGCGAAAATCTATTTTGAAGCTCTAGTGCCTTCCATCCCATCGTGGATGTTTG
TGGTGGCGCTGGTGGCCTTTATGACCGCCTTTAACCTGCGTAGTCTGAAATCCGTAGCGAACTTCAACACTGTAATCGTC
GTGTTGCAGGTAGTGCTGATCGCGGTGATTCTGGGCATGGTTGTTTATGGCGTATTTGAAGGTGAAGGCGCTGGTACGCT
GGCGAGCACTCGTCCGTTCTGGTCTGGCGATGCGCATGTTATCCCGATGATTACCGGGGCGACAATCCTGTGCTTCTCCT
TTACCGGCTTTGACGGCATCAGCAACCTGTCGGAAGAAACCAAAGATGCAGAGCGCGTGATCCCGCGTGCGATTTTCCTG
ACCGCGCTGATTGGCGGCATGATCTTCATCTTTGCAACTTACTTCCTGCAGCTGTACTTCCCGGATATCTCTCGCTTTAA
AGATCCGGATGCGTCACAGCCTGAAATCATGCTGTACGTTGCGGGTAAAGCGTTCCAGGTTGGCGCGCTGATCTTCTCCA
CCATTACCGTACTGGCGTCCGGTATGGCGGCGCATGCAGGCGTAGCGCGTCTGATGTACGTAATGGGTCGTGACGGCGTA
TTCCCGAAAAGCTTCTTCGGTTATGTACACCCGAAATGGCGTACTCCGGCGATGAACATCATCCTGGTTGGCGCGATTGC
CTTGCTGGCAATCAACTTCGACCTGGTAATGGCTACAGCGCTGATCAACTTTGGTGCGTTGGTGGCGTTCACCTTCGTTA
ACCTGTCGGTCATCTCGCAGTTCTGGATCCGTGAGAAGCGTAACAAGACGCTGAAAGATCACTTCCAGTATTTGTTCCTG
CCGATGTGTGGTGCGCTGACCGTTGGTGCGCTGTGGGTTAACCTGGAAGAAAGCTCAATGGTTCTGGGTCTGATCTGGGC
GGCTATCGGTCTGATTTACCTGGCTTGCGTCACCAAGAGTTTCCGTAACCCGGTTCCGCAGTACGAAGACGTAGCGTAA

Upstream 100 bases:

>100_bases
GATATTTACTAAAAATCGACAGTTGTTGTCGCTGAGGAATCCAAAAAAATGGGGCAATTTTTTGCTTACGCGACGGTTAT
CACCGTAAAGGAGAATGACC

Downstream 100 bases:

>100_bases
TCATCTTTTGTAATAATAAACCGGAGTCGAATGAATATTGGGCTCCGGTTTTTTTATTCTTTTTTATCGACATCCTCATT
AATAACCATTTGTTCTAATT

Product: amino acid permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVSGLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAG
SAYTYAQKSISPTVGFMVGWSSLLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFDGISNLSEETKDAERVIPRAIFL
TALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEIMLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGV
FPKSFFGYVHPKWRTPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLKDHFQYLFL
PMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYEDVA

Sequences:

>Translated_452_residues
MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVSGLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAG
SAYTYAQKSISPTVGFMVGWSSLLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFDGISNLSEETKDAERVIPRAIFL
TALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEIMLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGV
FPKSFFGYVHPKWRTPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLKDHFQYLFL
PMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYEDVA
>Mature_451_residues
SHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVSGLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGS
AYTYAQKSISPTVGFMVGWSSLLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIVV
LQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFDGISNLSEETKDAERVIPRAIFLT
ALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEIMLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVF
PKSFFGYVHPKWRTPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLKDHFQYLFLP
MCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYEDVA

Specific function: Probable amino-acid or metabolite transport protein

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily

Homologues:

Organism=Homo sapiens, GI4507047, Length=422, Percent_Identity=22.7488151658768, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI181337167, Length=401, Percent_Identity=23.4413965087282, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI186910308, Length=415, Percent_Identity=23.6144578313253, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI186910306, Length=415, Percent_Identity=23.6144578313253, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI186910304, Length=415, Percent_Identity=23.6144578313253, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI87082023, Length=452, Percent_Identity=100, Blast_Score=915, Evalue=0.0,
Organism=Escherichia coli, GI87081869, Length=446, Percent_Identity=63.677130044843, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI87082250, Length=328, Percent_Identity=24.390243902439, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790653, Length=351, Percent_Identity=21.9373219373219, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786789, Length=363, Percent_Identity=23.4159779614325, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786602, Length=271, Percent_Identity=23.9852398523985, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788480, Length=335, Percent_Identity=23.5820895522388, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17532491, Length=401, Percent_Identity=26.1845386533666, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17533459, Length=399, Percent_Identity=27.3182957393484, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17531343, Length=404, Percent_Identity=26.980198019802, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24667468, Length=408, Percent_Identity=26.9607843137255, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24666159, Length=422, Percent_Identity=23.4597156398104, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI221512776, Length=422, Percent_Identity=23.4597156398104, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24668806, Length=420, Percent_Identity=26.4285714285714, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356285, Length=420, Percent_Identity=26.4285714285714, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24668802, Length=420, Percent_Identity=26.4285714285714, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281366235, Length=352, Percent_Identity=24.7159090909091, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI116007820, Length=352, Percent_Identity=24.7159090909091, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI116007818, Length=352, Percent_Identity=24.7159090909091, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI85725152, Length=352, Percent_Identity=24.7159090909091, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YEEF_ECO57 (P0AA48)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- RefSeq:   NP_288519.1
- RefSeq:   NP_310843.1
- ProteinModelPortal:   P0AA48
- EnsemblBacteria:   EBESCT00000027926
- EnsemblBacteria:   EBESCT00000059659
- GeneID:   912852
- GeneID:   962072
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3176
- KEGG:   ecs:ECs2816
- GeneTree:   EBGT00050000009005
- HOGENOM:   HBG634400
- OMA:   WINLEES
- ProtClustDB:   CLSK866970
- BioCyc:   ECOL83334:ECS2816-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 49538; Mature: 49407

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c83cd18)-; HASH(0x1eaae9f8)-; HASH(0x1e6dc504)-; HASH(0x1d1d65e8)-; HASH(0x1eafaa1c)-; HASH(0x1ebc9adc)-; HASH(0x1db12c74)-; HASH(0x1e8582cc)-; HASH(0x1eaf6e60)-; HASH(0x1e31679c)-; HASH(0x1eb48e14)-; HASH(0x1e305000)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVSGLTDGHVPTAYAFALI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSSLLDYLFAPMINILLAKIY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIVVLQVVLIAVILGMVVYGVFE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFDGISNLSEETKDAERVIPRAIFL
CCCCCCCCCCCCCCCCCCEEEEEHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
TALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEIMLYVAGKAFQVGALIFSTITVLAS
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
GMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWRTPAMNIILVGAIALLAINFDLVMATA
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LINFGALVAFTFVNLSVISQFWIREKRNKTLKDHFQYLFLPMCGALTVGALWVNLEESSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCHH
VLGLIWAAIGLIYLACVTKSFRNPVPQYEDVA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure 
SHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVSGLTDGHVPTAYAFALI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSSLLDYLFAPMINILLAKIY
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIVVLQVVLIAVILGMVVYGVFE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFDGISNLSEETKDAERVIPRAIFL
CCCCCCCCCCCCCCCCCCEEEEEHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
TALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEIMLYVAGKAFQVGALIFSTITVLAS
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
GMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWRTPAMNIILVGAIALLAINFDLVMATA
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LINFGALVAFTFVNLSVISQFWIREKRNKTLKDHFQYLFLPMCGALTVGALWVNLEESSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCHH
VLGLIWAAIGLIYLACVTKSFRNPVPQYEDVA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796