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The map label for this gene is wcaK [H]

Identifier: 157156059

GI number: 157156059

Start: 2300047

End: 2301327

Strand: Reverse

Name: wcaK [H]

Synonym: EcE24377A_2337

Alternate gene names: 157156059

Gene position: 2301327-2300047 (Counterclockwise)

Preceding gene: 157156699

Following gene: 157158914

Centisome position: 46.21

GC content: 54.02

Gene sequence:

>1281_bases
ATGAAATTACTTATTCTGGGCAACCACACTTGCGGCAACCGCGGCGACAGCGCCATCCTGCGCGGCTTACTTGATGCCAT
CAACATCCTCAATCCACACGCCGAAGTGGACGTGATGAGCCGCTATCCGGTCAGTTCTTCCTGGCTGCTCAACCGCCCGG
TAATGGGCGATCCGCTGTTCCTGCAAATGAAACAACACAACAGCGCGGCGGGCGTTGTCGGGCGCGTTAAAAAAGTCCTC
CGTCGCCGCTATCAACACCAGGTATTGCTCTCACGCGTCACCGACACTGGCAAGCTGCGTAATATCGCCATCGCCCAGGG
ATTCACCGACTTCGTGCGCCTGCTCTCAGGTTACGACGCCATTATTCAGGTCGGCGGATCGTTTTTTGTCGATCTCTACG
GCGTGCCGCAGTTTGAACACGCGCTTTGCACGTTTATGGCGAAAAAGCCGCTGTTTATGATTGGTCACAGCGTCGGTCCC
TTCCAGGATGAGCAATTTAACCAACTGGCGAACTACGTTTTTGGTCACTGTGACGCGCTGATCCTACGCGAATCGGTCAG
CCTTGATTTGATGAAACGCAGCAATATCACTACCGCAAAAGTTGAACATGGCGTTGATACCGCGTGGCTGGTCGATCACC
ACACAGAAGATTTCACCGCCAGCTATGCCGTCCAACACTGGCTGGACGTTGCCGCACAACAGAAAACGGTGGCAATTACC
CTGCGCGAACTGGCACCGTTCGACAAACGTCTCGGCACCACTCAACAAGCGTATGAAAAAGCCTTTGCCGGGGTGGTCAA
TCGCATTCTCGACGAAGGCTATCAGGTCATTGCGCTCTCCACCTGTACGGGTATCGACAGCTATAACAAAGACGACCGCA
TGGTGGCGCTCAATCTTCGCCAGCACATCAGCGATCCTGCCCGTTACCATGTGGTGATGGATGAACTCAACGATCTGGAA
ATGGGCAAAATTCTCGGTGCCTGTGAACTCACCGTCGGTACGCGCCTGCACTCTGCCATTATCTCGATGAATTTTGCCAC
TCCGGCAATTGCCATCAACTACGAACATAAATCCGCCGGGATTATGCAGCAGCTGGGACTACCGGAGATGGCAATTGATA
TCCGTCATTTATTAGACGGCAGCCTGCAAGCGATGGTTGCGGATACCTTAGGCCAGCTTCCGGTGCTGAACGCTCGACTT
AACGAAGCCGTCAGTCGCGAACGTCAGACGGGAATGCAGATGGTGCAATCTGTGCTTGAACGCATCGGGGAGGTGAAATG
A

Upstream 100 bases:

>100_bases
CCTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCAAAAAGCGGCAACGAACAAAACAAACGCAGCCTCACCTGGC
ATCAAATTTATGAGGAAAAA

Downstream 100 bases:

>100_bases
AGGTCGGCTTCTTTTTACTGAAATTTCCGCTGTCGTCGGAAACCTTCGTTCTTAACCAAATTACCGCGTTTATTGATATG
GGATTCGAGGTGGAGATTGT

Product: putative pyruvyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPVSSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVL
RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLE
MGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARL
NEAVSRERQTGMQMVQSVLERIGEVK

Sequences:

>Translated_426_residues
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPVSSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVL
RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLE
MGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARL
NEAVSRERQTGMQMVQSVLERIGEVK
>Mature_426_residues
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPVSSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVL
RRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLE
MGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARL
NEAVSRERQTGMQMVQSVLERIGEVK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2327

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide pyruvyl transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788358, Length=426, Percent_Identity=99.2957746478873, Blast_Score=880, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007345 [H]

Pfam domain/function: PF04230 PS_pyruv_trans [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47365; Mature: 47365

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPVSSSWLLNRPVMGDPLF
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCCCCEECCCCCCCCEE
LQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCHH
IIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGPFQDEQFNQLANYVFGHCDAL
HHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
HHHHHCCHHHHHCCCCCEEEHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLR
HHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEHH
QHISDPARYHVVMDELNDLEMGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAG
HHCCCCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
IMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARLNEAVSRERQTGMQMVQSVLE
HHHHCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RIGEVK
HHCCCC
>Mature Secondary Structure
MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPVSSSWLLNRPVMGDPLF
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCCCCEECCCCCCCCEE
LQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNIAIAQGFTDFVRLLSGYDA
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHCCCHH
IIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGPFQDEQFNQLANYVFGHCDAL
HHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDFTASYAVQHWLDVAAQQKTVAIT
HHHHHCCHHHHHCCCCCEEEHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
LRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQVIALSTCTGIDSYNKDDRMVALNLR
HHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEHH
QHISDPARYHVVMDELNDLEMGKILGACELTVGTRLHSAIISMNFATPAIAINYEHKSAG
HHCCCCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
IMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQLPVLNARLNEAVSRERQTGMQMVQSVLE
HHHHCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RIGEVK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]