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The map label for this gene is nrfB

Identifier: 157163541

GI number: 157163541

Start: 4321011

End: 4321577

Strand: Direct

Name: nrfB

Synonym: EcHS_A4316

Alternate gene names: 157163541

Gene position: 4321011-4321577 (Clockwise)

Preceding gene: 157163540

Following gene: 157163542

Centisome position: 93.05

GC content: 53.97

Gene sequence:

>567_bases
ATGAGCGTATTACGTTCGTTATTAACTGCCGGGGTGCTGGCGTCGGGCCTGTTGTGGAGCCTGAACGGAATTACCGCTAC
CCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATA
AACCAGATACCGAAGGTATGCATGGAAAACATGCTTCCGTTATCAACCCGAATAACAAACTGCCGGTCACCTGCACCAAC
TGTCACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTGATGCGCTTTAACGAGCCGATGTACAAGGTTGG
AGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAA
CCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATT
AAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCA
GCCATGA

Upstream 100 bases:

>100_bases
CGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAACCCCGTTCCGCCTCGCAAGGGGCG
GAAAACACAATGGAGTGAAT

Downstream 100 bases:

>100_bases
CCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAA
ACGTTGAATATCAATGGGGT

Product: cytochrome c nitrite reductase pentaheme subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRNPDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTN
CHGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
KICVDCHSDQRTNPNFNPASVPLLKEQP

Sequences:

>Translated_188_residues
MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRNPDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTN
CHGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
KICVDCHSDQRTNPNFNPASVPLLKEQP
>Mature_187_residues
SVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRNPDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNC
HGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRIK
ICVDCHSDQRTNPNFNPASVPLLKEQP

Specific function: Plays a role in nitrite reduction

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=188, Percent_Identity=100, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFB_ECOL6 (P0ABL2)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_756919.1
- ProteinModelPortal:   P0ABL2
- SMR:   P0ABL2
- EnsemblBacteria:   EBESCT00000044459
- GeneID:   1038752
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c5067
- GeneTree:   EBGT00050000011179
- HOGENOM:   HBG678928
- OMA:   WTHDVHA
- ProtClustDB:   PRK11659
- GO:   GO:0006810
- InterPro:   IPR017564
- InterPro:   IPR011031
- TIGRFAMs:   TIGR03146

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 20715; Mature: 20583

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 49-49 BINDING 52-52 BINDING 78-78 BINDING 81-81 BINDING 113-113 BINDING 116-116 BINDING 138-138 BINDING 141-141 BINDING 163-163 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRNPDAACLDCHKPDTEGM
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCC
HGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPE
CCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCHH
QLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRIKICVDCHSDQRTNPNFNPAS
HHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
VPLLKEQP
CCCCCCCC
>Mature Secondary Structure 
SVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRNPDAACLDCHKPDTEGM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCC
HGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEPMYKVGEQNSVCMSCHLPE
CCCCCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCHH
QLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRIKICVDCHSDQRTNPNFNPAS
HHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
VPLLKEQP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157