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The map label for this gene is nrfC

Identifier: 157163542

GI number: 157163542

Start: 4321574

End: 4322245

Strand: Direct

Name: nrfC

Synonym: EcHS_A4317

Alternate gene names: 157163542

Gene position: 4321574-4322245 (Clockwise)

Preceding gene: 157163541

Following gene: 157163543

Centisome position: 93.07

GC content: 57.59

Gene sequence:

>672_bases
ATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGC
GAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGG
ATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCAGGGCGAATTT
CCTGATGTGAAATATCGCTTCTTCCGTAAGTCTTGCCAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGCCCGACCGG
TGCGTCTTTTCGTGATGCCGCTAGCGGGATTGTCGATGTTAATCCTGATCTCTGCGTCGGTTGTCAGTACTGCATCGCCG
CCTGTCCGTACCGCGTGCGCTTTATCCATCCGGTCACGAAGACGGCGGACAAATGCGATTTCTGCCGTAAAACCAATTTG
CAGGCCGGTAAGCTGCCCGCGTGCGTTGAGGCTTGCCCGACGAAGGCGCTGACGTTTGGCAATCTGGACGATCCTAACAG
TGAGATTTCGCAACTGCTGCGCCAGAAGCCTACTTACCGCTACAAGCTGGCGCTGGGAACCAAACCGAAGCTGTACCGCG
TACCGTTTAAATACGGGGAGGTGAGCCAATGA

Upstream 100 bases:

>100_bases
GTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTT
CCGTTGCTTAAGGAGCAGCC

Downstream 100 bases:

>100_bases
CGCAGACTTCCGCATTTCATTTTGAATCGCTGGTGTGGGACTGGCCGATTGCCATCTACCTGTTTTTGATTGGTATTTCT
GCCGGTCTGGTGACGCTGGC

Product: cytochrome c nitrite reductase, Fe-S protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEF
PDVKYRFFRKSCQHCDHAPCVDVCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKYGEVSQ

Sequences:

>Translated_223_residues
MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEF
PDVKYRFFRKSCQHCDHAPCVDVCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKYGEVSQ
>Mature_222_residues
TWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFP
DVKYRFFRKSCQHCDHAPCVDVCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNLQ
AGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKYGEVSQ

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI2367345, Length=223, Percent_Identity=100, Blast_Score=462, Evalue=1e-132,
Organism=Escherichia coli, GI226510944, Length=213, Percent_Identity=50.2347417840376, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1789370, Length=255, Percent_Identity=30.5882352941176, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787872, Length=188, Percent_Identity=33.5106382978723, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787122, Length=188, Percent_Identity=33.5106382978723, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787749, Length=170, Percent_Identity=30.5882352941176, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790326, Length=178, Percent_Identity=29.7752808988764, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082180, Length=167, Percent_Identity=32.3353293413174, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789067, Length=154, Percent_Identity=33.7662337662338, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1788811, Length=161, Percent_Identity=33.5403726708075, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787740, Length=111, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789079, Length=204, Percent_Identity=26.9607843137255, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787478, Length=104, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082179, Length=138, Percent_Identity=31.8840579710145, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI2367245, Length=173, Percent_Identity=31.7919075144509, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787963, Length=175, Percent_Identity=27.4285714285714, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI87082114, Length=199, Percent_Identity=26.6331658291457, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFC_ECO57 (P0AAK8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B86101
- PIR:   F91260
- RefSeq:   NP_290705.1
- RefSeq:   NP_313081.1
- ProteinModelPortal:   P0AAK8
- SMR:   P0AAK8
- EnsemblBacteria:   EBESCT00000026900
- EnsemblBacteria:   EBESCT00000055643
- GeneID:   914288
- GeneID:   960052
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5671
- KEGG:   ecs:ECs5054
- GeneTree:   EBGT00070000031694
- HOGENOM:   HBG726839
- OMA:   AYSMEDG
- ProtClustDB:   CLSK880840
- BioCyc:   ECOL83334:ECS5054-MONOMER
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311
- TIGRFAMs:   TIGR03149
- TIGRFAMs:   TIGR01409

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24568; Mature: 24436

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
7.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVN
CCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHH
KVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVDVCPTGASFRDAASGIVDV
HHHCCHHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEEC
NPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNLQAGKLPACVEACPTKALTFG
CCCHHHHHHHHHHHCCEEEEEECCHHHCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEC
NLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKYGEVSQ
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVN
CCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHH
KVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVDVCPTGASFRDAASGIVDV
HHHCCHHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEEC
NPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNLQAGKLPACVEACPTKALTFG
CCCHHHHHHHHHHHCCEEEEEECCHHHCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEC
NLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKYGEVSQ
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796