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The map label for this gene is yiaO

Identifier: 157163057

GI number: 157163057

Start: 3775397

End: 3776383

Strand: Direct

Name: yiaO

Synonym: EcHS_A3783

Alternate gene names: 157163057

Gene position: 3775397-3776383 (Clockwise)

Preceding gene: 157163056

Following gene: 157163058

Centisome position: 81.3

GC content: 51.06

Gene sequence:

>987_bases
ATGAAATTACGCTCTGTAACCTACGCATTATTCATTGCTGGCCTGGCTGCATTCAGCACATCTTCTCTGGCGGCACAATC
TTTACGTTTCGGTTATGAAACATCACAAACCGACTCGCAACATATTGCGGCGAAAAAATTCAATGATTTATTGCAGGAGA
GAACCAAAGGCGAGCTGAAATTAAAACTGTTCCCGGACAGCACTCTCGGTAACGCGCAGGCGATGATCAGCGGCGTACGT
GGCGGCACCATCGATATGGAAATGTCCGGCTCGAATAACTTTGCCGGGTTATCACCAGTGATGAACTTGCTTGATGTCCC
TTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAG
GTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCC
GCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCAATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCC
GATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCG
TCTGGTCAGCAAAATTTTTCGAAGTGCAGAAGTTCCTTTCTCTGACGCACCACGCCTATTCCCCGCTTCTGGTGGTGATC
AACAAAGCGAAGTTTGATGGCTTAAGTCCGGAGTTCCAGCAGGCGCTAGTTTCATCTGCACAAGAAGCGGGTAACTATCA
GCGCAAACTGGTTGCTGAAGATCAGCAAAAAATCATCGACGGCATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCG
ACCGCAAAGCCTTTAGCGACGCACTGGGGAATCAGGTTCGCGACATGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTG
CTGAAAGCCGTGGATGAGGTGCAATAA

Upstream 100 bases:

>100_bases
CCCTTACGTCCTGGTTTTATATTCATTATTAGTCGTATTTGTTTTTATTCCCGATCTCATCATCCTCCCTTTGAAATGGA
TTAATTAAAAGGAAAATATT

Downstream 100 bases:

>100_bases
ACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGA
GGCAGGCGTGCAGCGCCTGC

Product: TRAP transporter solute receptor DctP family protein

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVR
GGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQKFLSLTHHAYSPLLVVI
NKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADL
LKAVDEVQ

Sequences:

>Translated_328_residues
MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVR
GGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQKFLSLTHHAYSPLLVVI
NKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADL
LKAVDEVQ
>Mature_328_residues
MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVR
GGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQKFLSLTHHAYSPLLVVI
NKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADL
LKAVDEVQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family

Homologues:

Organism=Escherichia coli, GI1790004, Length=328, Percent_Identity=100, Blast_Score=674, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YIAO_ECOLI (P37676)

Other databases:

- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S47800
- RefSeq:   AP_004213.1
- RefSeq:   NP_418036.1
- ProteinModelPortal:   P37676
- SMR:   P37676
- STRING:   P37676
- EnsemblBacteria:   EBESCT00000003166
- EnsemblBacteria:   EBESCT00000003167
- EnsemblBacteria:   EBESCT00000017134
- GeneID:   948091
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3551
- KEGG:   eco:b3579
- EchoBASE:   EB2191
- EcoGene:   EG12283
- eggNOG:   COG1638
- GeneTree:   EBGT00050000010307
- HOGENOM:   HBG662660
- OMA:   AQTMISG
- ProtClustDB:   CLSK870418
- BioCyc:   EcoCyc:EG12283-MONOMER
- Genevestigator:   P37676
- GO:   GO:0006810
- InterPro:   IPR018389
- InterPro:   IPR004682
- TIGRFAMs:   TIGR00787

Pfam domain/function: PF03480 SBP_bac_7

EC number: NA

Molecular weight: Translated: 35970; Mature: 35970

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTL
EEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCC
DGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLKGLKIRTNNSPMNIAAF
CCCCCCCHHHCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEE
KVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQKFLSLTHHAYSPLLVVI
EEECCCCCCCCHHHHHCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEE
NKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSD
ECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALGNQVRDMFVKDVPQGADLLKAVDEVQ
HHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTL
EEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCC
DGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLKGLKIRTNNSPMNIAAF
CCCCCCCHHHCCCCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEE
KVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQKFLSLTHHAYSPLLVVI
EEECCCCCCCCHHHHHCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEE
NKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSD
ECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALGNQVRDMFVKDVPQGADLLKAVDEVQ
HHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503