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The map label for this gene is insK

Identifier: 157163034

GI number: 157163034

Start: 3750501

End: 3751352

Strand: Direct

Name: insK

Synonym: EcHS_A3760

Alternate gene names: 157163034

Gene position: 3750501-3751352 (Clockwise)

Preceding gene: 157163033

Following gene: 157163038

Centisome position: 80.77

GC content: 42.84

Gene sequence:

>852_bases
GTGAAAGTACTCAACGAGCTAAGGCAGTTTTATCCTCTTGATGAGCTTCTCAGGGCTGCGGAGATACCGCGCAGTACGTT
TTATTATCATCTAAAGGCTCTCAGCAAGCCTGACAAGTATGCGGACGTTAAAAAGCGTATTAGTGAGATTTATCACGAGA
ATAGAGGCCGATACGGATACCGTAGGGTAACGCTGTCTCTTCATCGAGAAGGGAAACAGATTAACCATAAAGCTGTTCAG
CGCCTGATGGGAACCCTCTCACTTAAAGCAGCGATTAAGGTCAAGCGATACCGCTCTTACAGAGGAGAGGTAGGGCAAAC
CGCCCCTAATGTTCTCCAAAGAGATTTCAAGGCTACGCGGCCAAACGAGAAGTGGGTTACCGATGTTACTGAATTTGCAG
TCAATGGGCGCAAGCTGTATTTGTCTCCAGTAATAGATCTCTTCAACAACGAAGTTATTTCTTACAGCCTTTCGGAAAGA
CCAGTGATGAACATGGTTGAGAATATGCTCGATCAGGCATTCAAAAAGCTTAATCCTCACGAGCATCCTGTTCTGCACTC
TGACCAGGGATGGCAGTATCGTATGAGAAGATATCAAAATATCCTTAAAGAACATGGTATTAAACAAAGCATGTCCAGAA
AAGGCAATTGTCTGGATAATGCTGTGGTGGAGTGTTTCTTTGGAACCTTAAAGTCGGAGTGTTTTTATCTTGATGAGTTC
AGTAATATAAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTACAACAGCAGAAGAATTAGCCTGAAATTAAA
AGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAA

Upstream 100 bases:

>100_bases
AAGACCGACGCATTGAGGATCTTGAAAGGCAAGTTCGATTTCTTGAAACGCGGCTTATGTATCTAAAAAAGCTGAAAGCC
TTAGCTCATCCCACGAAAAA

Downstream 100 bases:

>100_bases
CTGTCCAACTTTTTGGGGTCAGTACAAACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTGTCTGCCTTTCGGTGAATAC
CTGAGACAAACAGTCTCAAG

Product: IS150, transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQ
RLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSER
PVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV

Sequences:

>Translated_283_residues
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQ
RLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSER
PVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV
>Mature_283_residues
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQ
RLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSER
PVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV

Specific function: Involved in the transposition of the insertion sequence IS150

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain

Homologues:

Organism=Homo sapiens, GI310129419, Length=231, Percent_Identity=31.6017316017316, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1789981, Length=283, Percent_Identity=100, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1788405, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787263, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786753, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786570, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786490, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): INSK_ECOLI (P19769)

Other databases:

- EMBL:   X07037
- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_004235.1
- RefSeq:   NP_418015.1
- ProteinModelPortal:   P19769
- STRING:   P19769
- EnsemblBacteria:   EBESCT00000003593
- EnsemblBacteria:   EBESCT00000014325
- GeneID:   948081
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3528
- KEGG:   eco:b3558
- EcoGene:   EG40011
- eggNOG:   COG2801
- GeneTree:   EBGT00070000031687
- HOGENOM:   HBG757639
- OMA:   MESFFAV
- ProtClustDB:   CLSK862094
- BioCyc:   EcoCyc:G7777-MONOMER
- Genevestigator:   P19769
- GO:   GO:0032196
- InterPro:   IPR001584
- InterPro:   IPR012337

Pfam domain/function: PF00665 rve; SSF53098 RNaseH_fold

EC number: NA

Molecular weight: Translated: 33478; Mature: 33478

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRISEIYHENRGRYGY
CHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCH
RRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATR
HHEEHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPH
CCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCC
EHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEHHHH
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCC
>Mature Secondary Structure
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRISEIYHENRGRYGY
CHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCH
RRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATR
HHEEHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPH
CCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCC
EHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEHHHH
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503