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The map label for this gene is insJ

Identifier: 157163033

GI number: 157163033

Start: 3749983

End: 3750504

Strand: Direct

Name: insJ

Synonym: EcHS_A3759

Alternate gene names: 157163033

Gene position: 3749983-3750504 (Clockwise)

Preceding gene: 157163031

Following gene: 157163034

Centisome position: 80.76

GC content: 46.17

Gene sequence:

>522_bases
ATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACTACTTCACAACTGATGATGGTTACAGGAT
CATCTCGGCACGTTTTGGTGTCCCCCGAACCCAGGTCAGGACATGGGTTGCCCTCTATGAAAAACATGGAGAAAAAGGTT
TAATTCCCAAACCTAAAGGCGTTAGTGCTGATCCAGAGTTGCGTATTAAGGTCGTGAAAGCTGTGATCGAGCAGCACATG
TCCCTTAATCAGGCTGCTGCTCACTTTATGCTTGCTGGTAGTGGTTCTGTAGCCAGGTGGCTGAAGGTCTATGAAGAGCG
CGGAGAAGCTGGTTTACGCGCGCTCAAGATTGGCACCAAAAGAAACATTGCAATATCAGTTGATCCAGAAAAAGCGGCAT
CAGCATTGGAGCTGTCAAAAGACCGACGCATTGAGGATCTTGAAAGGCAAGTTCGATTTCTTGAAACGCGGCTTATGTAT
CTAAAAAAGCTGAAAGCCTTAGCTCATCCCACGAAAAAGTGA

Upstream 100 bases:

>100_bases
TAATCTATATTTCTGCGGCATTCCCAGCCTCCTGTGTTGATTTCCAACGAGTATGTACTGCACCCATTTTGTTGGACGAT
GAAATGGAATAGCCCCTAAT

Downstream 100 bases:

>100_bases
AAGTACTCAACGAGCTAAGGCAGTTTTATCCTCTTGATGAGCTTCTCAGGGCTGCGGAGATACCGCGCAGTACGTTTTAT
TATCATCTAAAGGCTCTCAG

Product: IS150, transposase orfA

Products: NA

Alternate protein names: ORFA

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHM
SLNQAAAHFMLAGSGSVARWLKVYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
LKKLKALAHPTKK

Sequences:

>Translated_173_residues
MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHM
SLNQAAAHFMLAGSGSVARWLKVYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
LKKLKALAHPTKK
>Mature_172_residues
SKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMS
LNQAAAHFMLAGSGSVARWLKVYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMYL
KKLKALAHPTKK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS150/IS1296 orfA family

Homologues:

Organism=Escherichia coli, GI1789980, Length=173, Percent_Identity=100, Blast_Score=353, Evalue=5e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): INSJ_ECOLI (P19768)

Other databases:

- EMBL:   X07037
- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S47778
- RefSeq:   AP_004236.1
- RefSeq:   NP_065293.1
- RefSeq:   NP_418014.1
- ProteinModelPortal:   P19768
- STRING:   P19768
- PRIDE:   P19768
- EnsemblBacteria:   EBESCT00000003281
- EnsemblBacteria:   EBESCT00000017102
- GeneID:   1238726
- GeneID:   948082
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3527
- KEGG:   eco:b3557
- EchoBASE:   EB4746
- EcoGene:   EG40010
- eggNOG:   COG2963
- GeneTree:   EBGT00050000008260
- HOGENOM:   HBG756815
- OMA:   HYLRLEV
- ProtClustDB:   CLSK862093
- BioCyc:   EcoCyc:G7776-MONOMER
- Genevestigator:   P19768
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 19725; Mature: 19594

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPKG
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
VSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLKVYEERGEAGLRALKIGTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCHHHHHHEEECCC
RNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMYLKKLKALAHPTKK
CCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVALYEKHGEKGLIPKPKG
CCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
VSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLKVYEERGEAGLRALKIGTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCHHHHHHEEECCC
RNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMYLKKLKALAHPTKK
CCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503