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The map label for this gene is dtpB

Identifier: 157162972

GI number: 157162972

Start: 3683373

End: 3684842

Strand: Direct

Name: dtpB

Synonym: EcHS_A3696

Alternate gene names: 157162972

Gene position: 3683373-3684842 (Clockwise)

Preceding gene: 157162971

Following gene: 157162975

Centisome position: 79.32

GC content: 51.77

Gene sequence:

>1470_bases
ATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCG
ATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTG
TCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACC
AAACGCACCATTGTTCTTGGTGCACTTGTGCTGGCGATTGGCTACTTCATGACCGGCATGTCGCTACTTAAGCCTGACCT
GATTTTCATCGCCCTGGGGACTATCGCTGTAGGTAACGGCCTGTTTAAAGCTAACCCAGCCAGCTTGCTTTCGAAGTGCT
ATCCGCCGAAAGATCCGCGGCTTGATGGCGCATTCACCCTGTTCTATATGTCGATCAACATCGGCTCGTTGATAGCGTTA
TCGCTGGCCCCTGTGATCGCTGATAGATTCGGTTATTCAGTCACCTACAACCTGTGCGGGGCGGGGTTAATTATCGCATT
ACTGGTTTACATCGCCTGTCGTGGAATGGTGAAAGACATTGGTTCTGAACCCGACTTCCGGCCAATGAGCTTCAGCAAAC
TGTTGTACGTGTTACTTGGCAGCGTGGTGATGATCTTCGTATGCGCATGGCTGATGCACAACGTAGAAGTCGCCAATCTG
GTGCTGATTGTTCTCTCCATCGTCGTCACCATCATCTTCTTTCGTCAGGCATTCAAGCTGGATAAAACCGGGCGCAATAA
AATGTTTGTCGCCTTTGTCCTGATGCTCGAAGCGGTGGTGTTTTACATTCTCTACGCCCAGATGCCAACATCGCTGAACT
TCTTTGCCATCAACAACGTGCATCATGAAATTCTCGGTTTTTCCATCAACCCGGTCAGCTTCCAGGCGCTTAACCCGTTC
TGGGTGGTACTCGCCAGCCCAATACTGGCAGGCATTTACACGCATCTGGGTAACAAAGGCAAAGACCTCTCGATGCCGAT
GAAATTTACTCTCGGCATGTTTATGTGCTCACTGGGCTTTTTGACGGCGGCAGCTGCGGGAATGTGGTTTGCGGATGCAC
AAGGGCTGACATCGCCATGGTTTATCGTGCTGGTGTACTTATTCCAGAGCTTAGGTGAACTGTTTATTAGCGCCCTTGGC
CTGGCGATGATTGCTGCCCTGGTGCCGCAGCATTTGATGGGCTTTATTCTCGGGATGTGGTTCCTGACGCAGGCTGCCGC
GTTCTTGCTGGGCGGCTATGTGGCAACATTTACCGCGGTGCCGGACAACATTACCGATCCGCTTGAGACGTTGCCCGTCT
ATACCAACGTGTTTGGTAAGATTGGTCTGGTCACGCTGGGCGTTGCAGTAGTGATGCTGTTGATGGTGCCGTGGCTGAAA
CGCATGATTGCGACGCCGGAAAGCCATTAA

Upstream 100 bases:

>100_bases
ACCGCATCAATATTAAGAATCTCTGACAGATGTAAACTTTTTCGCGCGTTATCCCTTACGCGTTCATACTTTTCAGGATG
GTATTGGAAGGTTAATAAAT

Downstream 100 bases:

>100_bases
TTATTCTTGCAGAAAGCAGGGTAGCGTTATCGCTACCCTGTTTTAGTTTTACACTGGCGTACCTGCATAGATATCAAAGC
GATGCCCTTTAGTGACTACC

Product: inner membrane transporter YhiP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQLGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGT
KRTIVLGALVLAIGYFMTGMSLLKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVLLGSVVMIFVCAWLMHNVEVANL
VLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLMLEAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPF
WVVLASPILAGIYTHLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLGELFISALG
LAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLETLPVYTNVFGKIGLVTLGVAVVMLLMVPWLK
RMIATPESH

Sequences:

>Translated_489_residues
MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQLGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGT
KRTIVLGALVLAIGYFMTGMSLLKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVLLGSVVMIFVCAWLMHNVEVANL
VLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLMLEAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPF
WVVLASPILAGIYTHLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLGELFISALG
LAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLETLPVYTNVFGKIGLVTLGVAVVMLLMVPWLK
RMIATPESH
>Mature_489_residues
MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQLGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGT
KRTIVLGALVLAIGYFMTGMSLLKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVLLGSVVMIFVCAWLMHNVEVANL
VLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLMLEAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPF
WVVLASPILAGIYTHLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLGELFISALG
LAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLETLPVYTNVFGKIGLVTLGVAVVMLLMVPWLK
RMIATPESH

Specific function: Proton-dependent permease that transports di- and tripeptides. Has a clear preference for dipeptides and tripeptides composed of L-amino acids, and discriminates dipeptides on the basis of the position of charges within the substrate

COG id: COG3104

COG function: function code E; Dipeptide/tripeptide permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTR2/POT transporter (TC 2.A.17) family. DtpB subfamily

Homologues:

Organism=Homo sapiens, GI4827008, Length=384, Percent_Identity=22.65625, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI226371746, Length=193, Percent_Identity=29.5336787564767, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1789911, Length=489, Percent_Identity=100, Blast_Score=980, Evalue=0.0,
Organism=Escherichia coli, GI1787922, Length=481, Percent_Identity=52.1829521829522, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1786927, Length=481, Percent_Identity=27.027027027027, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1790572, Length=486, Percent_Identity=27.1604938271605, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17541704, Length=376, Percent_Identity=25.531914893617, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571098, Length=212, Percent_Identity=26.8867924528302, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571102, Length=212, Percent_Identity=26.8867924528302, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571100, Length=212, Percent_Identity=26.8867924528302, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24639583, Length=329, Percent_Identity=24.6200607902736, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24639585, Length=329, Percent_Identity=24.6200607902736, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24639581, Length=329, Percent_Identity=24.6200607902736, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DTPB_ECOLI (P36837)

Other databases:

- EMBL:   U00039
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C65147
- RefSeq:   AP_004297.1
- RefSeq:   NP_417953.1
- ProteinModelPortal:   P36837
- DIP:   DIP-12369N
- MINT:   MINT-1286392
- STRING:   P36837
- EnsemblBacteria:   EBESCT00000004170
- EnsemblBacteria:   EBESCT00000017608
- GeneID:   948006
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3463
- KEGG:   eco:b3496
- EchoBASE:   EB2144
- EcoGene:   EG12232
- eggNOG:   COG3104
- GeneTree:   EBGT00050000010038
- HOGENOM:   HBG586603
- OMA:   RMINTPD
- ProtClustDB:   PRK10207
- BioCyc:   EcoCyc:YHIP-MONOMER
- Genevestigator:   P36837
- HAMAP:   MF_01879
- InterPro:   IPR016196
- InterPro:   IPR000109
- InterPro:   IPR005279
- InterPro:   IPR018456
- PANTHER:   PTHR11654:SF6
- TIGRFAMs:   TIGR00924

Pfam domain/function: PF00854 PTR2; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 53576; Mature: 53576

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS01022 PTR2_1; PS01023 PTR2_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x17d992b8)-; HASH(0x183d218c)-; HASH(0x17f31aec)-; HASH(0x17a00258)-; HASH(0x175ca27c)-; HASH(0x16d21540)-; HASH(0x1749ab94)-; HASH(0x18322578)-; HASH(0x1450c5b8)-; HASH(0x1833e804)-; HASH(0x17ae1598)-; HASH(0xe8387d4)-; HASH(0x18310d14)-; HASH(0x181f8d54)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQLGFSQEQAFVTFGAFA
CCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSLLKPDLIFIALGTIAVGNG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
LFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIALSLAPVIADRFGYSVTYNLCG
CCCCCHHHHHHHCCCCCCCCCCCCEEEEEEHHCHHHHHHHHHHHHHHHHCCCEEEHHHHH
AGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVLLGSVVMIFVCAWLMHNVEVANL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLMLEAVVFYILYAQMPTSLNFFAINNV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCH
HHEILGFSINPVSFQALNPFWVVLASPILAGIYTHLGNKGKDLSMPMKFTLGMFMCSLGF
HHHHHCCCCCCCEEHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LTAAAAGMWFADAQGLTSPWFIVLVYLFQSLGELFISALGLAMIAALVPQHLMGFILGMW
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLTQAAAFLLGGYVATFTAVPDNITDPLETLPVYTNVFGKIGLVTLGVAVVMLLMVPWLK
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMIATPESH
HHHCCCCCH
>Mature Secondary Structure
MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQLGFSQEQAFVTFGAFA
CCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSLLKPDLIFIALGTIAVGNG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
LFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIALSLAPVIADRFGYSVTYNLCG
CCCCCHHHHHHHCCCCCCCCCCCCEEEEEEHHCHHHHHHHHHHHHHHHHCCCEEEHHHHH
AGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVLLGSVVMIFVCAWLMHNVEVANL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLMLEAVVFYILYAQMPTSLNFFAINNV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCH
HHEILGFSINPVSFQALNPFWVVLASPILAGIYTHLGNKGKDLSMPMKFTLGMFMCSLGF
HHHHHCCCCCCCEEHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LTAAAAGMWFADAQGLTSPWFIVLVYLFQSLGELFISALGLAMIAALVPQHLMGFILGMW
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLTQAAAFLLGGYVATFTAVPDNITDPLETLPVYTNVFGKIGLVTLGVAVVMLLMVPWLK
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMIATPESH
HHHCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503