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The map label for this gene is uspA [H]

Identifier: 157162971

GI number: 157162971

Start: 3682621

End: 3683055

Strand: Direct

Name: uspA [H]

Synonym: EcHS_A3695

Alternate gene names: 157162971

Gene position: 3682621-3683055 (Clockwise)

Preceding gene: 157162969

Following gene: 157162972

Centisome position: 79.31

GC content: 49.2

Gene sequence:

>435_bases
ATGGCTTATAAACACATTCTCATCGCGGTCGATCTCTCCCCGGAAAGCAAAGTTCTGGTAGAGAAAGCAGTCTCTATGGC
TCGCCCCTACAATGCGAAAGTTTCTCTGATCCACGTAGATGTAAACTACTCTGACCTATACACCGGGCTTATTGATGTGA
ATCTGGGTGATATGCAGAAACGCATCTCAGAAGAGACACATCATGCGCTGACCGAGCTTTCCACTAATGCAGGCTACCCA
ATCACTGAAACCCTGAGCGGCAGCGGCGACCTGGGCCAAGTTCTGGTCGATGCAATCAAGAAATACGATATGGATCTGGT
GGTTTGTGGTCACCACCAGGACTTCTGGAGCAAACTGATGTCTTCCGCACGTCAGCTGATCAACACCGTTCACGTTGATA
TGCTGATTGTTCCGCTGCGCGACGAAGAAGAATAA

Upstream 100 bases:

>100_bases
GGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGACGTAACGGCACAAGAAACGCTAGCTGGCCAGTCATCGACAAC
TTTATGGAAGGAGTAACACT

Downstream 100 bases:

>100_bases
TCTTCCCTCTACGACGTGTTCCTGAACGCCCGCATATGCGGGCGTTTTGCTTTTTGGCGCGCCTTGTTACCTGATCCAGC
GTAAACACCTTATCTGGCCT

Product: universal stress protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYSDLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYP
ITETLSGSGDLGQVLVDAIKKYDMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE

Sequences:

>Translated_144_residues
MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYSDLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYP
ITETLSGSGDLGQVLVDAIKKYDMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE
>Mature_143_residues
AYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYSDLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPI
TETLSGSGDLGQVLVDAIKKYDMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE

Specific function: Required for resistance to DNA-damaging agents [H]

COG id: COG0589

COG function: function code T; Universal stress protein UspA and related nucleotide-binding proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the universal stress protein A family [H]

Homologues:

Organism=Escherichia coli, GI1789909, Length=144, Percent_Identity=100, Blast_Score=295, Evalue=8e-82,
Organism=Escherichia coli, GI1788205, Length=140, Percent_Identity=38.5714285714286, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI1790358, Length=142, Percent_Identity=35.9154929577465, Blast_Score=100, Evalue=3e-23,

Paralogues:

None

Copy number: 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1580 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 290 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4003 Molecules/Cell In: Growth Phase, G

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR006016 [H]

Pfam domain/function: PF00582 Usp [H]

EC number: NA

Molecular weight: Translated: 16067; Mature: 15935

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYSDLYTGLIDVNLGDMQK
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHEEEEEECCHHHHHH
RISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKYDMDLVVCGHHQDFWSKLM
HHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
SSARQLINTVHVDMLIVPLRDEEE
HHHHHHHHHHEEEEEEEECCCCCC
>Mature Secondary Structure 
AYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYSDLYTGLIDVNLGDMQK
CCEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHEEEEEECCHHHHHH
RISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKYDMDLVVCGHHQDFWSKLM
HHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
SSARQLINTVHVDMLIVPLRDEEE
HHHHHHHHHHEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]