Click here to switch to the map view.

The map label for this gene is gspF1

Identifier: 157162801

GI number: 157162801

Start: 3504160

End: 3505356

Strand: Direct

Name: gspF1

Synonym: EcHS_A3521

Alternate gene names: 157162801

Gene position: 3504160-3505356 (Clockwise)

Preceding gene: 157162800

Following gene: 157162802

Centisome position: 75.46

GC content: 51.04

Gene sequence:

>1197_bases
ATGAATTATCGCTATCGCGCCATGACCCAGGATGGTCAAAAATTGCAAGGGATCATTGATGCTAACGATGAACGTCAGGC
ACGACTGCGGCTGCGTGAAGAAGGGCTTTTCCTGCTGGATATTCGCCCCCAAAAAAGTTCGGGAGTAAAAACACGTCGCC
CGAGGATCAGCCATAGTGAACTGACGCTTTTCACCCGGCAGTTGGCAACCTTAAGCGCAGCGGCATTACCCCTGGAAGAG
AGCCTTGCCGTAATCGGTCAACAAAGCAGTAATAAACGACTGGGTGACGTGTTAAATCAGGTACGCAGCGCCATCCTTGA
AGGGCATCCCCTTTCCGATGCATTACAGCATTTTCCCACGCTTTTCGATTCGCTCTATCGTACCCTGGTAAAAGCGGGCG
AAAAGAGCGGGCTGCTGGCCCCGGTGTTGGAAAAGCTGGCTGATTACAATGAAAACCGGCAGAAAATCCGCAGCAAGCTC
ATTCAGTCACTGATCTACCCCTGTATGCTCACTACGGTGGCGATTGGGGTCGTGATTATTCTCCTCACTGCTGTCGTGCC
CAAAATTACCGAACAGTTCGTGCATATGAAGCAGCAACTGCCGCTGAGTACACGCATTCTTTTAGGTCTGAGCGACACGT
TGCAACGTACCGGCCCGACATTATTAGCGACAGTGTTTATTGTCGCTGTAGGTTTCTGGCTCTGGTTAAAACGCGGCAAT
AACCGCCACCGTTTTCATGCCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTA
TCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACA
ACCTCGAAATTCGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAA
ACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAG
AGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTA
CGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAA

Upstream 100 bases:

>100_bases
ACAAGGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAGGTTATGCGCGTCGCCAGT
GCCACGCTGGAGAGCGAAGC

Downstream 100 bases:

>100_bases
GGAAAGCATATGCGCGCAACGGATAAGCAACGCGGTTTTACATTACTGGAAATTATGGTGGTGATCGTCATTATCGGCGT
ACTTGCCAGCCTGGTGGTTC

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Protein transport protein hofF

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQKSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEE
SLAVIGQQSSNKRLGDVLNQVRSAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTGPTLLATVFIVAVGFWLWLKRGN
NRHRFHAMLLRVALIGPLICAINSARYLRTLSILQSSGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQ
TAIFPPMMLYMVASGEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQLNSMIN

Sequences:

>Translated_398_residues
MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQKSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEE
SLAVIGQQSSNKRLGDVLNQVRSAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTGPTLLATVFIVAVGFWLWLKRGN
NRHRFHAMLLRVALIGPLICAINSARYLRTLSILQSSGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQ
TAIFPPMMLYMVASGEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQLNSMIN
>Mature_398_residues
MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQKSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEE
SLAVIGQQSSNKRLGDVLNQVRSAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTGPTLLATVFIVAVGFWLWLKRGN
NRHRFHAMLLRVALIGPLICAINSARYLRTLSILQSSGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQ
TAIFPPMMLYMVASGEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQLNSMIN

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family

Homologues:

Organism=Escherichia coli, GI1789724, Length=398, Percent_Identity=100, Blast_Score=803, Evalue=0.0,
Organism=Escherichia coli, GI1786295, Length=350, Percent_Identity=24.5714285714286, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSPF_ECOLI (P41441)

Other databases:

- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   U20786
- PIR:   B65126
- RefSeq:   AP_004463.1
- RefSeq:   NP_417786.1
- ProteinModelPortal:   P41441
- STRING:   P41441
- EnsemblBacteria:   EBESCT00000000352
- EnsemblBacteria:   EBESCT00000000353
- EnsemblBacteria:   EBESCT00000000354
- EnsemblBacteria:   EBESCT00000015601
- GeneID:   947829
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3289
- KEGG:   eco:b3327
- EchoBASE:   EB2722
- EcoGene:   EG12885
- eggNOG:   COG1459
- GeneTree:   EBGT00050000009906
- HOGENOM:   HBG635225
- OMA:   VIEQFVH
- ProtClustDB:   CLSK891804
- BioCyc:   EcoCyc:G7705-MONOMER
- Genevestigator:   P41441
- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992
- PRINTS:   PR00812
- TIGRFAMs:   TIGR02120

Pfam domain/function: PF00482 GSPII_F

EC number: NA

Molecular weight: Translated: 44340; Mature: 44340

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12e4f0c4)-; HASH(0x1796da9c)-; HASH(0x11f893c4)-; HASH(0x17dfcb6c)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQKSSGVKTRRPRISHSE
CCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHH
LTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVRSAILEGHPLSDALQHFPT
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKLIQSLIYPCMLTTVAIGVVII
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTGPTLLATVFIVAVGFWLWLKRGN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
NRHRFHAMLLRVALIGPLICAINSARYLRTLSILQSSGVPLLDGMNLSTESLNNLEIRQR
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHH
LANAAENVRQGNSIHLSLEQTAIFPPMMLYMVASGEKSGQLGTLMVRAADNQETLQQNRI
HHHHHHHHHCCCEEEEEEHHHHCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCE
ALTLSIFEPALIITMALIVLFIVVSVLQPLLQLNSMIN
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQKSSGVKTRRPRISHSE
CCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHH
LTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVRSAILEGHPLSDALQHFPT
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKLIQSLIYPCMLTTVAIGVVII
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTGPTLLATVFIVAVGFWLWLKRGN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
NRHRFHAMLLRVALIGPLICAINSARYLRTLSILQSSGVPLLDGMNLSTESLNNLEIRQR
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHH
LANAAENVRQGNSIHLSLEQTAIFPPMMLYMVASGEKSGQLGTLMVRAADNQETLQQNRI
HHHHHHHHHCCCEEEEEEHHHHCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHCCE
ALTLSIFEPALIITMALIVLFIVVSVLQPLLQLNSMIN
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896718