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The map label for this gene is gspE1

Identifier: 157162800

GI number: 157162800

Start: 3502682

End: 3504163

Strand: Direct

Name: gspE1

Synonym: EcHS_A3520

Alternate gene names: 157162800

Gene position: 3502682-3504163 (Clockwise)

Preceding gene: 157162799

Following gene: 157162801

Centisome position: 75.43

GC content: 52.56

Gene sequence:

>1482_bases
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGTCAGTGCCAGACCATGACCAGTTTGT
CACAGGCTGATTTCGAAGCCCGGCTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAAGGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Upstream 100 bases:

>100_bases
GAAATCAAAAGCGCTGGTTGGCTCGGAAGATTTGCCGGTGCTGGATGAAAACACGTTCAACAGTCACGCCCCTGCGCCAT
CGTCACGGTGAGGCATTCAT

Downstream 100 bases:

>100_bases
ATTATCGCTATCGCGCCATGACCCAGGATGGTCAAAAATTGCAAGGGATCATTGATGCTAACGATGAACGTCAGGCACGA
CTGCGGCTGCGTGAAGAAGG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Sequences:

>Translated_493_residues
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA
>Mature_493_residues
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family

Homologues:

Organism=Escherichia coli, GI1789723, Length=493, Percent_Identity=100, Blast_Score=1008, Evalue=0.0,
Organism=Escherichia coli, GI1786296, Length=390, Percent_Identity=43.8461538461538, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI87082188, Length=279, Percent_Identity=35.84229390681, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSPE_ECOLI (P45759)

Other databases:

- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A65126
- RefSeq:   AP_004464.1
- RefSeq:   NP_417785.1
- ProteinModelPortal:   P45759
- SMR:   P45759
- IntAct:   P45759
- STRING:   P45759
- EnsemblBacteria:   EBESCT00000004070
- EnsemblBacteria:   EBESCT00000004071
- EnsemblBacteria:   EBESCT00000004072
- EnsemblBacteria:   EBESCT00000018038
- GeneID:   947823
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3288
- KEGG:   eco:b3326
- EchoBASE:   EB2728
- EcoGene:   EG12891
- eggNOG:   COG2804
- GeneTree:   EBGT00050000009768
- HOGENOM:   HBG485518
- OMA:   WAKSQRI
- ProtClustDB:   CLSK891803
- BioCyc:   EcoCyc:G7704-MONOMER
- Genevestigator:   P45759
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482
- SMART:   SM00382
- TIGRFAMs:   TIGR02533

Pfam domain/function: PF00437 GSPII_E

EC number: NA

Molecular weight: Translated: 54611; Mature: 54611

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEA
CCCCCCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALLISRIKVMARLDIAEKRIPQD
HHHHHCCEEEEECCEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
CCEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHC
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSF
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHH
ARGLRAILRQDPDVVMVGEIRDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGV
HHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCH
ESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFKYHQLAVTTIGTPVGCPHCHQ
HHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCHHC
SGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
CCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VMRVASATLESEA
HHHHHHHHHCCCC
>Mature Secondary Structure
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEA
CCCCCCCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALLISRIKVMARLDIAEKRIPQD
HHHHHCCEEEEECCEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
GRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
CCEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHC
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSF
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHH
ARGLRAILRQDPDVVMVGEIRDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGV
HHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCH
ESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFKYHQLAVTTIGTPVGCPHCHQ
HHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCHHC
SGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
CCCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VMRVASATLESEA
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503