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The map label for this gene is degQ

Identifier: 157162712

GI number: 157162712

Start: 3424331

End: 3425698

Strand: Direct

Name: degQ

Synonym: EcHS_A3423

Alternate gene names: 157162712

Gene position: 3424331-3425698 (Clockwise)

Preceding gene: 157162711

Following gene: 157162713

Centisome position: 73.74

GC content: 52.05

Gene sequence:

>1368_bases
ATGAAAAAACAAACCCAGCTGTTGAGTGCATTAGCGTTAAGTGTCGGGTTAACTCTCTCGGCGTCATTTCAGGCCGTCGC
GTCGATTCCAGGCCAGGTTGCCGATCAGGCCCCTCTCCCCAGTCTGGCTCCAATGCTGGAAAAAGTGCTTCCGGCAGTGG
TGAGCGTACGGGTGGAAGGAACGGCCAGTCAGGGACAGAAAATCCCGGAAGAATTCAAAAAGTTTTTTGGTGATGATTTA
CCGGATCAACCTGCACAACCCTTCGAAGGTTTAGGCTCCGGTGTCATCATCAACGCCAGTAAAGGCTATGTGCTGACCAA
CAACCATGTGATTAATCAGGCACAGAAAATCAGTATTCAGCTCAATGATGGGCGCGAGTTTGATGCAAAACTGATTGGTA
GCGATGACCAGAGCGATATCGCCCTGTTACAAATTCAAAACCCGAGCAAATTAACGCAAATCGCTATTGCCGACTCCGAT
AAATTGCGCGTCGGTGATTTTGCCGTAGCGGTCGGTAACCCATTTGGCCTTGGGCAAACCGCCACCTCTGGCATTGTTTC
CGCATTAGGCCGCAGCGGGTTGAATCTTGAAGGTCTGGAAAACTTTATCCAGACAGATGCTTCCATTAACCGCGGTAACT
CCGGCGGTGCACTATTAAACCTTAACGGTGAGTTAATTGGCATCAACACTGCAATCCTTGCGCCTGGCGGCGGGAGCGTC
GGGATTGGATTTGCCATCCCCAGTAATATGGCGCGAACACTGGCGCAGCAGCTTATCGACTTTGGTGAAATCAAACGCGG
TTTGTTAGGCATCAAAGGCACCGAGATGAGTGCCGATATCGCCAAAGCCTTCAACCTTGACGTGCAGCGTGGCGCGTTTG
TCAGCGAAGTGTTGCCAGGTTCTGGCTCGGCAAAAGCGGGCGTCAAAGCGGGCGATATTATTACCAGCCTCAACGGCAAA
CCGCTGAATAGCTTTGCTGAGTTGCGCTCTCGTATCGCGACCACCGAGCCGGGCACGAAAGTGAAGCTTGGCCTGCTGCG
TAACGGCAAACCACTGGAAGTAGAAGTGACGCTCGATACCAGCACCTCTTCGTCGGCCAGCGCTGAAATGATCACGCCAG
CGCTGGAAGGTGCAACGTTGAGCGATGGTCAGCTAAAAGATGGCGGCAAAGGTATTAAAATCGATGAAGTTGTCAAAGGA
AGCCCAGCTGCTCAGGCTGGCTTGCAAAAAGACGATGTGATCATTGGCGTCAACCGCGATCGGGTGAACTCGATTGCTGA
AATGCGTAAAGTGCTGGCGGCAAAACCGGCCATCATCGCCCTGCAAATTGTACGCGGCAATGAAAGCATCTATCTGCTGA
TGCGTTAA

Upstream 100 bases:

>100_bases
TCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATT
CAGGTACGAGAGCAGGAATA

Downstream 100 bases:

>100_bases
TGTCGTAAACCGGGCATCAGGCTTACGTGTGATGTCCGGTTAACTCGTGGTATGCTGCTGCCGTTCCCTTTTTTAATGAC
GCCTCCATCATGTTTGTGAA

Product: serine endoprotease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAPMLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDL
PDQPAQPFEGLGSGVIINASKGYVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGALLNLNGELIGINTAILAPGGGSV
GIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEMSADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGK
PLNSFAELRSRIATTEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGIKIDEVVKG
SPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESIYLLMR

Sequences:

>Translated_455_residues
MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAPMLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDL
PDQPAQPFEGLGSGVIINASKGYVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGALLNLNGELIGINTAILAPGGGSV
GIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEMSADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGK
PLNSFAELRSRIATTEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGIKIDEVVKG
SPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESIYLLMR
>Mature_455_residues
MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAPMLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDL
PDQPAQPFEGLGSGVIINASKGYVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGALLNLNGELIGINTAILAPGGGSV
GIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEMSADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGK
PLNSFAELRSRIATTEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGIKIDEVVKG
SPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESIYLLMR

Specific function: Protease with a shared specificity with degP

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PDZ (DHR) domains

Homologues:

Organism=Homo sapiens, GI22129776, Length=316, Percent_Identity=36.3924050632911, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4506141, Length=294, Percent_Identity=35.7142857142857, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI24308541, Length=305, Percent_Identity=30.8196721311475, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI7019477, Length=296, Percent_Identity=33.7837837837838, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1789629, Length=455, Percent_Identity=100, Blast_Score=890, Evalue=0.0,
Organism=Escherichia coli, GI1786356, Length=440, Percent_Identity=57.9545454545455, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1789630, Length=271, Percent_Identity=44.6494464944649, Blast_Score=209, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24646839, Length=289, Percent_Identity=32.5259515570934, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEGQ_ECOLI (P39099)

Other databases:

- EMBL:   U15661
- EMBL:   U32495
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   JC6051
- RefSeq:   AP_003776.1
- RefSeq:   NP_417701.1
- ProteinModelPortal:   P39099
- SMR:   P39099
- DIP:   DIP-9424N
- MINT:   MINT-1246722
- STRING:   P39099
- MEROPS:   S01.274
- SWISS-2DPAGE:   P39099
- 2DBase-Ecoli:   P39099
- EnsemblBacteria:   EBESCT00000002944
- EnsemblBacteria:   EBESCT00000014642
- GeneID:   947812
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3203
- KEGG:   eco:b3234
- EchoBASE:   EB2496
- EcoGene:   EG12612
- eggNOG:   COG0265
- GeneTree:   EBGT00050000010522
- HOGENOM:   HBG585708
- OMA:   IQIADSD
- ProtClustDB:   PRK10139
- BioCyc:   EcoCyc:G7682-MONOMER
- BioCyc:   MetaCyc:G7682-MONOMER
- Genevestigator:   P39099
- GO:   GO:0006508
- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011782
- InterPro:   IPR001254
- InterPro:   IPR001940
- PRINTS:   PR00834
- SMART:   SM00228
- TIGRFAMs:   TIGR02037

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin; SSF50156 PDZ; SSF50494 Pept_Ser_Cys

EC number: 3.4.21.-

Molecular weight: Translated: 47206; Mature: 47206

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50106 PDZ

Important sites: ACT_SITE 109-109 ACT_SITE 139-139 ACT_SITE 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAPMLEKVLPAVVSVRVEG
CCHHHHHHHHHHHHHCCEEECCHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHEEEEEEC
TASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKGYVLTNNHVINQAQKISIQ
CHHCCCCCHHHHHHHHCCCCCCCCCCCHHHCCCCEEEECCCCEEEECCCEECCEEEEEEE
LNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSDKLRVGDFAVAVGNPFGLGQT
ECCCCCCCEEEECCCCCCCEEEEEECCCCCEEEEEEECCCCEEECEEEEEECCCCCCCCH
ATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGALLNLNGELIGINTAILAPGGGSV
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEEEEEEEECCCCCE
GIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEMSADIAKAFNLDVQRGAFVSEVLPG
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCCC
SGSAKAGVKAGDIITSLNGKPLNSFAELRSRIATTEPGTKVKLGLLRNGKPLEVEVTLDT
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEEEC
STSSSASAEMITPALEGATLSDGQLKDGGKGIKIDEVVKGSPAAQAGLQKDDVIIGVNRD
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHCCCCCCCEEEECCHH
RVNSIAEMRKVLAAKPAIIALQIVRGNESIYLLMR
HHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAPMLEKVLPAVVSVRVEG
CCHHHHHHHHHHHHHCCEEECCHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHEEEEEEC
TASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKGYVLTNNHVINQAQKISIQ
CHHCCCCCHHHHHHHHCCCCCCCCCCCHHHCCCCEEEECCCCEEEECCCEECCEEEEEEE
LNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSDKLRVGDFAVAVGNPFGLGQT
ECCCCCCCEEEECCCCCCCEEEEEECCCCCEEEEEEECCCCEEECEEEEEECCCCCCCCH
ATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGALLNLNGELIGINTAILAPGGGSV
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEEEEEEEECCCCCE
GIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEMSADIAKAFNLDVQRGAFVSEVLPG
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCCC
SGSAKAGVKAGDIITSLNGKPLNSFAELRSRIATTEPGTKVKLGLLRNGKPLEVEVTLDT
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEEEC
STSSSASAEMITPALEGATLSDGQLKDGGKGIKIDEVVKGSPAAQAGLQKDDVIIGVNRD
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHCCCCCCCEEEECCHH
RVNSIAEMRKVLAAKPAIIALQIVRGNESIYLLMR
HHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8576051; 9278503