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The map label for this gene is agaV [H]

Identifier: 157162616

GI number: 157162616

Start: 3328837

End: 3329310

Strand: Direct

Name: agaV [H]

Synonym: EcHS_A3323

Alternate gene names: 157162616

Gene position: 3328837-3329310 (Clockwise)

Preceding gene: 157162615

Following gene: 157162617

Centisome position: 71.69

GC content: 50.84

Gene sequence:

>474_bases
ATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGG
GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAG
AAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTG
CTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATAT
GCACTACGCCAATGGCAAACAACAAATCGCCAAAACGGTTTCTGTGGATGCGGGCGATATCGCAGCATTTAACGACCTGA
AAGCCGCTGGGGTGGAATGCTTCGTTCAGGGCGTCCCGACAGAGCCTGCTGTGGACCTCTTTAAATTACTTTGA

Upstream 100 bases:

>100_bases
CCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGC
AAAACAAAGAGGAACACGCT

Downstream 100 bases:

>100_bases
GGGATTCATCATGGAAATCAGCCTGTTGCAGGCATTTGCGTTGGGCATTATCGCCTTTATCGCTGGCCTGGATATGTTTA
ACGGCCTAACACATATGCAC

Product: N-acetylgalactosamine-specific PTS system transporter subunit IIB

Products: NA

Alternate protein names: EIIB-Aga'; PTS system N-acetylgalactosamine-specific EIIB component 2 [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKIL
LVCKTPADFLTLVKGGVPVNRINVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL

Sequences:

>Translated_157_residues
MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKIL
LVCKTPADFLTLVKGGVPVNRINVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL
>Mature_156_residues
PNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILL
VCKTPADFLTLVKGGVPVNRINVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI87082217, Length=157, Percent_Identity=99.3630573248408, Blast_Score=317, Evalue=3e-88,
Organism=Escherichia coli, GI1789527, Length=146, Percent_Identity=47.945205479452, Blast_Score=133, Evalue=6e-33,
Organism=Escherichia coli, GI1788120, Length=157, Percent_Identity=38.2165605095541, Blast_Score=107, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17056; Mature: 16925

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNLMEMVLAEGIAVRF
CCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCHHCCHHHHHHHHHHHHCCHHHHH
WTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVGNMHYANGKQQIAKTV
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCEEEEECCCEEECCCHHHHHHHH
SVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL
CCCCCCCHHHHCHHHHCHHHEEECCCCCHHHHHHHHC
>Mature Secondary Structure 
PNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNLMEMVLAEGIAVRF
CCHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCHHCCHHHHHHHHHHHHCCHHHHH
WTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVGNMHYANGKQQIAKTV
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCEEEEECCCEEECCCHHHHHHHH
SVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL
CCCCCCCHHHHCHHHHCHHHEEECCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 8932697 [H]