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The map label for this gene is exuT

Identifier: 157162570

GI number: 157162570

Start: 3288777

End: 3290075

Strand: Direct

Name: exuT

Synonym: EcHS_A3276

Alternate gene names: 157162570

Gene position: 3288777-3290075 (Clockwise)

Preceding gene: 157162566

Following gene: 157162575

Centisome position: 70.82

GC content: 54.35

Gene sequence:

>1299_bases
ATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAA
CACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCT
ATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATG
TTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCTTGGCTGTTGCTCGTGGTGC
GGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCA
TCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATG
CACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATCTGGGCGATGGCATGGCTGATTTTCTATAAACA
TCCGCGCGACCAGAAGCATCTGACCGATGAAGAACGCGACTATATTATTAATGGTCAGGAAGCCCAGCACCAGGTGAGCA
CGGCGAAGAAAATGTCCGTTGGTCAGATCCTGCGTAACCGTCAGTTCTGGGGTATCGCGCTGCCGCGTTTCCTGGCAGAA
CCGGCCTGGGGTACTTTTAACGCGTGGATCCCGCTGTTCATGTTTAAAGTTTACGGCTTTAACCTGAAAGAGATCGCGAT
GTTCGCCTGGATGCCGATGCTGTTTGCTGACCTCGGTTGTATCCTCGGTGGTTACCTGCCGCCGCTGTTCCAGCGTTGGT
TTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGC
CTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGAT
TACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCGAACGGTTTAACCGGGATGTCCGCATGGCTGGCAA
GTACGCTATTTGCGCTGGTTGTCGGTGCACTGGCTGACACCATCGGCTTCAGCCCGCTGTTCGCAGTACTGGCAGTGTTC
GACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGA
TCCCGCACCGCAACATTAA

Upstream 100 bases:

>100_bases
CCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATA
ACAAAACGATGAGGTTTTAC

Downstream 100 bases:

>100_bases
CATTTAACGTAAGTTGTAATGACGAAGCCGCCTTATCAGGCGGCTTCGTTGTTTATATAGACAAAAGCGTTTGTCCGGTT
TGCAGGGTTAATTGATGGTT

Product: putative hexuronate transporter

Products: galacturonate [Cytoplasm]; Proton [Cytoplasm]; glucuronate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNISTQQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAM
FAVLWAVFCGATALAGSWGGLAVARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKMSVGQILRNRQFWGIALPRFLAE
PAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFADLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIG
LFTNPYVAIMLLCIGGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSPLFAVLAVF
DLLGALVIWTVLQNKPAIEVAQETHNDPAPQH

Sequences:

>Translated_432_residues
MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNISTQQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAM
FAVLWAVFCGATALAGSWGGLAVARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKMSVGQILRNRQFWGIALPRFLAE
PAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFADLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIG
LFTNPYVAIMLLCIGGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSPLFAVLAVF
DLLGALVIWTVLQNKPAIEVAQETHNDPAPQH
>Mature_432_residues
MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNISTQQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAM
FAVLWAVFCGATALAGSWGGLAVARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKMSVGQILRNRQFWGIALPRFLAE
PAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFADLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIG
LFTNPYVAIMLLCIGGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSPLFAVLAVF
DLLGALVIWTVLQNKPAIEVAQETHNDPAPQH

Specific function: Aldohexuronate transport system

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Phthalate permease family

Homologues:

Organism=Homo sapiens, GI6912666, Length=383, Percent_Identity=23.2375979112272, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI148743791, Length=256, Percent_Identity=25, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI154800443, Length=409, Percent_Identity=23.7163814180929, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI2367193, Length=432, Percent_Identity=100, Blast_Score=871, Evalue=0.0,
Organism=Escherichia coli, GI87082320, Length=410, Percent_Identity=26.8292682926829, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI2367379, Length=310, Percent_Identity=28.3870967741935, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789515, Length=410, Percent_Identity=26.0975609756098, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789152, Length=350, Percent_Identity=24, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87082071, Length=441, Percent_Identity=23.3560090702948, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17552542, Length=318, Percent_Identity=24.2138364779874, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533556, Length=270, Percent_Identity=23.3333333333333, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115533554, Length=270, Percent_Identity=23.3333333333333, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24580790, Length=243, Percent_Identity=24.6913580246914, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550460, Length=412, Percent_Identity=25.2427184466019, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24655255, Length=382, Percent_Identity=28.0104712041885, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24639764, Length=456, Percent_Identity=22.1491228070175, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20129235, Length=360, Percent_Identity=25.2777777777778, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641682, Length=367, Percent_Identity=23.433242506812, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24658658, Length=301, Percent_Identity=24.2524916943522, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28573708, Length=316, Percent_Identity=23.1012658227848, Blast_Score=66, Evalue=6e-11,
Organism=Drosophila melanogaster, GI28573707, Length=316, Percent_Identity=23.1012658227848, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EXUT_ECO57 (P0AA79)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F85970
- PIR:   G91125
- RefSeq:   NP_289667.1
- RefSeq:   NP_312002.1
- ProteinModelPortal:   P0AA79
- EnsemblBacteria:   EBESCT00000025446
- EnsemblBacteria:   EBESCT00000058883
- GeneID:   916191
- GeneID:   958081
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4446
- KEGG:   ecs:ECs3975
- GeneTree:   EBGT00050000008818
- HOGENOM:   HBG338043
- OMA:   ASTMFAL
- ProtClustDB:   CLSK880579
- BioCyc:   ECOL83334:ECS3975-MONOMER
- InterPro:   IPR004744
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- TIGRFAMs:   TIGR00893

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 46893; Mature: 46893

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x177f7d1c)-; HASH(0x17869ac0)-; HASH(0x1787dbbc)-; HASH(0x178a3360)-; HASH(0x178b5c3c)-; HASH(0x178e18c0)-; HASH(0x178814b0)-; HASH(0x1787757c)-; HASH(0x1768d7cc)-; HASH(0x178801e0)-; HASH(0x17869bb0)-; HASH(0x178b5ca8)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNISTQQYSYIIAAYSAAYTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLAVARGAVGAAEAAMIPAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHCCCCC
KASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVMHSWQMAFIISGALSFIWAMA
CCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKMSVGQILRNRQFWGIALPRFLAE
HHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
PAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFADLGCILGGYLPPLFQRWFGVNLIV
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCIGGFAHQALSGALITLSSDVFGRNEVA
HHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
TANGLTGMSAWLASTLFALVVGALADTIGFSPLFAVLAVFDLLGALVIWTVLQNKPAIEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AQETHNDPAPQH
HHHCCCCCCCCH
>Mature Secondary Structure
MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNISTQQYSYIIAAYSAAYTV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLAVARGAVGAAEAAMIPAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHCCCCC
KASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVMHSWQMAFIISGALSFIWAMA
CCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKMSVGQILRNRQFWGIALPRFLAE
HHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
PAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFADLGCILGGYLPPLFQRWFGVNLIV
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCIGGFAHQALSGALITLSSDVFGRNEVA
HHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCC
TANGLTGMSAWLASTLFALVVGALADTIGFSPLFAVLAVFDLLGALVIWTVLQNKPAIEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AQETHNDPAPQH
HHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: galacturonate [Periplasm]; Proton [Periplasm]; glucuronate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + galacturonate [Periplasm] = galacturonate [Cytoplasm] + Proton [Cytoplasm] Proton [Periplasm] + glucuronate [Periplasm] = Proton [Cytoplasm] + glucuronate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796