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The map label for this gene is ebgR [H]

Identifier: 157162551

GI number: 157162551

Start: 3264910

End: 3265893

Strand: Direct

Name: ebgR [H]

Synonym: EcHS_A3257

Alternate gene names: 157162551

Gene position: 3264910-3265893 (Clockwise)

Preceding gene: 157162549

Following gene: 157162552

Centisome position: 70.31

GC content: 51.83

Gene sequence:

>984_bases
ATGGCAACACTAAAAGACATCGCAATCGAAGCTGGCGTATCCCTGGCGACAGTATCCAGGGTCTTAAATGACGATCCGAC
ATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGCGCCCGTA
AACTACAGACAGGCGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCT
TACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAG
CGGATTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCG
CGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGC
ATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGCATTGGTTTTATTGGCGGTGAAGATGAGCCTGG
CAAGGCGGATATTCGTGAGGTCGCCTTTGTGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCG
GCGGTTTTTCCAGTTCGTCGGGCTATGAACTGGCAAAACAGATGCTGTCGCGGGAAGACTATCCGAAGGCACTGTTTGTT
GCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGTGGCCTGAACATCCCACAGGATATTTCGCTTAT
CAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAA
GTCAGGGCGTTAACCTGGTGTATGAAAAAGCCCGCGATGGTCGCGCGCTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTA
AAACTGCGCGGCACGACCCGTTAA

Upstream 100 bases:

>100_bases
CAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAGGTGACCCAATAAGCACTTCCTCTACAATGGAAGCGCACATCAG
GGAAAGTAAAAAAGGTAAAC

Downstream 100 bases:

>100_bases
ATCCCCTTACACACTGTCCGGCAGTCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGC
ATTTAACTGCTACTTTTTAG

Product: DNA-binding transcriptional repressor EbgR

Products: NA

Alternate protein names: Ebg operon repressor [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDP
YYLAIRHGIETQCEKLGIELTNCYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFVEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLSREDYPKALFV
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKL
KLRGTTR

Sequences:

>Translated_327_residues
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDP
YYLAIRHGIETQCEKLGIELTNCYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFVEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLSREDYPKALFV
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKL
KLRGTTR
>Mature_326_residues
ATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPY
YLAIRHGIETQCEKLGIELTNCYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARI
SKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFVEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLSREDYPKALFVA
SDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLK
LRGTTR

Specific function: Repressor for beta galactosidase alpha and beta subunits (ebgA and ebgC). Binds lactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=327, Percent_Identity=99.388379204893, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=28.7425149700599, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=27.3529411764706, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=26.813880126183, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788474, Length=347, Percent_Identity=25.6484149855908, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=26.2536873156342, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789068, Length=303, Percent_Identity=26.7326732673267, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36255; Mature: 36124

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV
CCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
NQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTNCYEHSGLPDIKNVTGILI
CCCEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHCCCCEEHHHHCCCCCCCCCCEEEE
VGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVNRIGFI
ECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
GGEDEPGKADIREVAFVEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLSREDYPKALFV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEE
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLV
ECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCCCEE
YEKARDGRALPLLVFVPSKLKLRGTTR
EECCCCCCCCEEEEECCCCEEECCCCC
>Mature Secondary Structure 
ATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV
CCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
NQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTNCYEHSGLPDIKNVTGILI
CCCEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHCCCCEEHHHHCCCCCCCCCCEEEE
VGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVNRIGFI
ECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
GGEDEPGKADIREVAFVEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLSREDYPKALFV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEE
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLV
ECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCCCEE
YEKARDGRALPLLVFVPSKLKLRGTTR
EECCCCCCCCEEEEECCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2515108; 3939708; 9278503 [H]