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The map label for this gene is ygjG [H]

Identifier: 157162549

GI number: 157162549

Start: 3263028

End: 3264317

Strand: Direct

Name: ygjG [H]

Synonym: EcHS_A3255

Alternate gene names: 157162549

Gene position: 3263028-3264317 (Clockwise)

Preceding gene: 157162546

Following gene: 157162551

Centisome position: 70.27

GC content: 55.19

Gene sequence:

>1290_bases
ATGAAAGCACTTAACCGAGAGGTGATTAAATACTTCAAAGAGCATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGT
TACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGAAGTTTAAATACGCTTGTCGACACCCAGGGCCAGGAGT
TTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCACCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAA
CTTGCGAAACAACCGCTGCACAGCCAGGAGCTGCTCGATCCGTTACGGGCGATGTTGGCGAAAACCCTTGCTGCGCTAAC
GCCCGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACCGAGTCCGTTGAAGCAGCGCTGAAGCTGGCGAAAGCTT
ACCAGTCACCGCGCGGCAAGTTTACTTTTATTGCCACCAGCGGCGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCC
ACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGC
CATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAG
GTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTC
GATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCGCCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTG
CCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCG
ACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTG
CTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATA
TCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGATGGCGATTGAGTTTGTTGATAACGAAATCGGCTATAACT
TTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCA
CTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGA
AGAAGCGTAA

Upstream 100 bases:

>100_bases
GGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGC
GAACGCTGGATCATGAGGAG

Downstream 100 bases:

>100_bases
TATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACGACGCGTACGCCCGCAGGCATCATCCGCTCCGG
CGTTAACAGCACGCTTTCAC

Product: putrescine--2-oxoglutarate aminotransferase

Products: NA

Alternate protein names: Putrescine--2-oxoglutaric acid transaminase; PAT; PATase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKALNREVIKYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQ
LAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSA
TAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMIL
DEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVL
LEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPP
LTLTIEQCELVIKAARKALAAMRVSVEEA

Sequences:

>Translated_429_residues
MKALNREVIKYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQ
LAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSA
TAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMIL
DEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVL
LEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPP
LTLTIEQCELVIKAARKALAAMRVSVEEA
>Mature_429_residues
MKALNREVIKYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQ
LAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSA
TAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMIL
DEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVL
LEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPP
LTLTIEQCELVIKAARKALAAMRVSVEEA

Specific function: Catalyzes the aminotransferase reaction from putrescine to 2-oxoglutarate, leading to glutamate and 4-aminobutanal, which spontaneously cyclizes to form 1-pyrroline. Is also able to transaminate cadaverine and, in lower extent, spermidine, but not ornithi

COG id: COG4992

COG function: function code E; Ornithine/acetylornithine aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. Putrescine aminotransferase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=384, Percent_Identity=33.59375, Blast_Score=212, Evalue=7e-55,
Organism=Homo sapiens, GI284507298, Length=312, Percent_Identity=35.8974358974359, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI13994255, Length=460, Percent_Identity=30.4347826086957, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI37574042, Length=405, Percent_Identity=27.6543209876543, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI226442705, Length=405, Percent_Identity=27.4074074074074, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI226442709, Length=385, Percent_Identity=27.7922077922078, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI24119277, Length=410, Percent_Identity=26.8292682926829, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI188536080, Length=433, Percent_Identity=23.7875288683603, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI38679950, Length=433, Percent_Identity=23.7875288683603, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI38679946, Length=433, Percent_Identity=23.7875288683603, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI145693181, Length=429, Percent_Identity=99.7668997668998, Blast_Score=880, Evalue=0.0,
Organism=Escherichia coli, GI1789759, Length=330, Percent_Identity=36.6666666666667, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1788044, Length=374, Percent_Identity=34.4919786096257, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787560, Length=403, Percent_Identity=34.9875930521092, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789016, Length=338, Percent_Identity=34.3195266272189, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1786991, Length=394, Percent_Identity=31.2182741116751, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1786349, Length=334, Percent_Identity=29.3413173652695, Blast_Score=125, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25144271, Length=369, Percent_Identity=33.6043360433604, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI25144274, Length=260, Percent_Identity=38.4615384615385, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI32564660, Length=433, Percent_Identity=28.175519630485, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71992977, Length=419, Percent_Identity=27.6849642004773, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17541228, Length=440, Percent_Identity=23.4090909090909, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=389, Percent_Identity=35.9897172236504, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6323470, Length=390, Percent_Identity=30.5128205128205, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6324386, Length=320, Percent_Identity=29.6875, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321456, Length=429, Percent_Identity=23.5431235431235, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357415, Length=385, Percent_Identity=34.2857142857143, Blast_Score=211, Evalue=7e-55,
Organism=Drosophila melanogaster, GI21356575, Length=413, Percent_Identity=31.4769975786925, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI28574759, Length=449, Percent_Identity=28.7305122494432, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI161085790, Length=456, Percent_Identity=28.7280701754386, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI281366494, Length=310, Percent_Identity=26.4516129032258, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24667139, Length=310, Percent_Identity=26.4516129032258, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24667143, Length=310, Percent_Identity=26.4516129032258, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR010164
- InterPro:   IPR017747
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.82 [H]

Molecular weight: Translated: 46400; Mature: 46400

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALNREVIKYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGG
CCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCCHHHHHHHCC
FGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSG
CCEEECCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
TESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHV
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCC
PFGNIEAMRTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMIL
CCCCHHHHHHHHHHHHHCCCHHEEHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEE
DEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHT
HHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEE
TTFGGNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGML
EECCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
MAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
EEEEECCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCEEEEHHHHHHHHHHHHHHHH
AMRVSVEEA
HHHHHHCCC
>Mature Secondary Structure
MKALNREVIKYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGG
CCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCCHHHHHHHCC
FGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSG
CCEEECCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
TESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHV
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCC
PFGNIEAMRTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMIL
CCCCHHHHHHHHHHHHHCCCHHEEHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEE
DEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHT
HHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEE
TTFGGNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGML
EECCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
MAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
EEEEECCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCEEEEHHHHHHHHHHHHHHHH
AMRVSVEEA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA