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The map label for this gene is spaP [H]

Identifier: 157162320

GI number: 157162320

Start: 3021684

End: 3022349

Strand: Reverse

Name: spaP [H]

Synonym: EcHS_A3018

Alternate gene names: 157162320

Gene position: 3022349-3021684 (Counterclockwise)

Preceding gene: 157162321

Following gene: 157162319

Centisome position: 65.09

GC content: 34.53

Gene sequence:

>666_bases
ATGTCAAATAGCATTTCGCTGATAGCTATACTATCATTATTTACACTATTGCCGTTTATAATAGCATCCGGGACATATTT
TATTAAATTCTCGATTGTTTTTGTTATTGTCCGAAACGCGCTGGGGCTTCAGCAAGTGCCATCAAATATGACACTTAATG
GGGTTGCTTTGCTATTGTCAATGTTTGTAATGATGCCGGTTGGGAAGGAAATATATTATAACAGTCAGAATGAGAATCTT
TCATTCAATAATTTAGCGTCGGTTGTTAATTTTGTTGAGACAGGCATGAGTGGTTATAAATCATATTTGATAAAATATTC
CGAACCGGAGTTGGTTAACTTTTTTGAAAAGATTCAGAAGGTGAATAGCTCTGAAGACAATGAAGAGATATTCGATGATG
ATAATATTTCAATTTTCTCGTTACTCCCGGCATATGCGTTGAGTGAGATAAAAAGTGCATTTATTATAGGTTTTTATATC
TATTTACCCTTTGTTGTAGTGGATCTGGTTATATCCATCGTTCTGTTAACGTTAGGTATGATGATGATGAGTCCGGTAAC
CATATCTACACCGATAAAATTAATTTTGTTTGTCGCGATGGACGGTTGGACAATGTTATCAAAAGGACTCATTTTACAAT
ATTTTGACCTATCCATTAATCCCTGA

Upstream 100 bases:

>100_bases
GAAATACGTGTAAATGGCGCGCTAACTGGCTATGGAGAACTTGTTGAAGTGGATGATAAATTGGGCGTAGAGATTCATTC
TTGGTTATCAGGGCATAATA

Downstream 100 bases:

>100_bases
GAAAAAAATATGGACGATATTGTTTTTGCGGGGAATAGGGCGCTGTATCTGATTTTAGTTATGTCTGCAGGACCTATTGC
AGTTGCGACATTTGTTGGTT

Product: surface presentation of antigens protein SpaP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQQVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENL
SFNNLASVVNFVETGMSGYKSYLIKYSEPELVNFFEKIQKVNSSEDNEEIFDDDNISIFSLLPAYALSEIKSAFIIGFYI
YLPFVVVDLVISIVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP

Sequences:

>Translated_221_residues
MSNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQQVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENL
SFNNLASVVNFVETGMSGYKSYLIKYSEPELVNFFEKIQKVNSSEDNEEIFDDDNISIFSLLPAYALSEIKSAFIIGFYI
YLPFVVVDLVISIVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP
>Mature_220_residues
SNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQQVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLS
FNNLASVVNFVETGMSGYKSYLIKYSEPELVNFFEKIQKVNSSEDNEEIFDDDNISIFSLLPAYALSEIKSAFIIGFYIY
LPFVVVDLVISIVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP

Specific function: Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells [H]

COG id: COG4790

COG function: function code U; Type III secretory pathway, component EscR

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliP/mopC/spaP family [H]

Homologues:

Organism=Escherichia coli, GI1788259, Length=215, Percent_Identity=35.8139534883721, Blast_Score=127, Evalue=4e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005838
- InterPro:   IPR005773 [H]

Pfam domain/function: PF00813 FliP [H]

EC number: NA

Molecular weight: Translated: 24736; Mature: 24604

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS01061 FLIP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQQVPSNMTLNGVALLLS
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHH
MFVMMPVGKEIYYNSQNENLSFNNLASVVNFVETGMSGYKSYLIKYSEPELVNFFEKIQK
HHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VNSSEDNEEIFDDDNISIFSLLPAYALSEIKSAFIIGFYIYLPFVVVDLVISIVLLTLGM
HCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP
HHHCCCCCCCHHHHHHHHHHCCHHHHHCCHHEEEECCCCCC
>Mature Secondary Structure 
SNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQQVPSNMTLNGVALLLS
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHH
MFVMMPVGKEIYYNSQNENLSFNNLASVVNFVETGMSGYKSYLIKYSEPELVNFFEKIQK
HHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VNSSEDNEEIFDDDNISIFSLLPAYALSEIKSAFIIGFYIYLPFVVVDLVISIVLLTLGM
HCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP
HHHCCCCCCCHHHHHHHHHHCCHHHHHCCHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7638176; 11677608; 12644504 [H]