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The map label for this gene is epaO [H]

Identifier: 157162321

GI number: 157162321

Start: 3022339

End: 3023286

Strand: Reverse

Name: epaO [H]

Synonym: EcHS_A3019

Alternate gene names: 157162321

Gene position: 3023286-3022339 (Counterclockwise)

Preceding gene: 157162322

Following gene: 157162320

Centisome position: 65.11

GC content: 35.02

Gene sequence:

>948_bases
ATGTTCGGTCTGAGGAAAGTAAATCGAAACACTCATACGTTTGAAACCACTTTTCAAAATTGGAAAGAAAATGGCGAAGA
TGTTGCATTATTAATGCCCGAATTTAGTGCTAAATGGCTACCGATAGCAGAAGAGAGTGGCAGCTGGTCTGGATGGGTAT
TGCTTGGAGAAATATTTCCCTTAATTTCATCTGAGCTCGCTGGGATGGCTTTAATGCCAGAAACGGAAAGGTTAATAGGG
GAATGGCTCAGTTTATCCAGTTCCCCGTTAAATCTAAAGCATCCTGAACTAAAATATAATCGTTTGCGCGTAGGTAAAGT
ATTTGATGGAGTATTGAGCTCTGCTCAACCACTAATAAGAATATGGACAGGGGAATTGAATCTTTGGTTAGATAAAGTCA
CAGTCTGCCAATACGGAAACGCTCCAGCGTTAGACAAAAAATCGTTATATTGCTCCATTCATTTTGTAATTGGATTTAGT
AAAACATGTTACAGAAGTCTTGTTGATATTGAAGTTGGTGATGTTTTATTAATCTCAAATAATTTGGCTTATGCGGTTAT
TTATAATACAAAAATTTTTGATTTAATTTATCCAGAGGAGTTAAAAATGGCTGATCATTTTGAGTACGAGGAAGATTTTG
AAACAGATGATTTTGATATCAAAAAAAACGAGAGTGAGATTTATGATGAAAATGACGATCAGATGATTAATAGTTTTGAA
GACTTGCCTGTAAAAATTGAGTTTGTTCTTGGTAAGAAAATAATGAATCTATATGAAATAGACGAACTCTGTGCAAAAAG
AATAATATCTCTGCTTTCTGAATCTGAGAAAAATATAGAAATACGTGTAAATGGCGCGCTAACTGGCTATGGAGAACTTG
TTGAAGTGGATGATAAATTGGGCGTAGAGATTCATTCTTGGTTATCAGGGCATAATAATGTCAAATAG

Upstream 100 bases:

>100_bases
TATTGACTGAGCAAGATGAACGACAAGGACAACGACATCAGCCTTATGAGGAGCAAGAAAATGAAAGCAAATTCGAAAAC
GATCAGAAGGATGAAAGTTA

Downstream 100 bases:

>100_bases
CATTTCGCTGATAGCTATACTATCATTATTTACACTATTGCCGTTTATAATAGCATCCGGGACATATTTTATTAAATTCT
CGATTGTTTTTGTTATTGTC

Product: surface presentation of antigens protein SpaO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAEESGSWSGWVLLGEIFPLISSELAGMALMPETERLIG
EWLSLSSSPLNLKHPELKYNRLRVGKVFDGVLSSAQPLIRIWTGELNLWLDKVTVCQYGNAPALDKKSLYCSIHFVIGFS
KTCYRSLVDIEVGDVLLISNNLAYAVIYNTKIFDLIYPEELKMADHFEYEEDFETDDFDIKKNESEIYDENDDQMINSFE
DLPVKIEFVLGKKIMNLYEIDELCAKRIISLLSESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGHNNVK

Sequences:

>Translated_315_residues
MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAEESGSWSGWVLLGEIFPLISSELAGMALMPETERLIG
EWLSLSSSPLNLKHPELKYNRLRVGKVFDGVLSSAQPLIRIWTGELNLWLDKVTVCQYGNAPALDKKSLYCSIHFVIGFS
KTCYRSLVDIEVGDVLLISNNLAYAVIYNTKIFDLIYPEELKMADHFEYEEDFETDDFDIKKNESEIYDENDDQMINSFE
DLPVKIEFVLGKKIMNLYEIDELCAKRIISLLSESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGHNNVK
>Mature_315_residues
MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAEESGSWSGWVLLGEIFPLISSELAGMALMPETERLIG
EWLSLSSSPLNLKHPELKYNRLRVGKVFDGVLSSAQPLIRIWTGELNLWLDKVTVCQYGNAPALDKKSLYCSIHFVIGFS
KTCYRSLVDIEVGDVLLISNNLAYAVIYNTKIFDLIYPEELKMADHFEYEEDFETDDFDIKKNESEIYDENDDQMINSFE
DLPVKIEFVLGKKIMNLYEIDELCAKRIISLLSESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGHNNVK

Specific function: Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells [H]

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001543
- InterPro:   IPR003283
- InterPro:   IPR013385 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36035; Mature: 36035

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAEESGSWSGWVLLGEIFP
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEHHHHHH
LISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRLRVGKVFDGVLSSAQPLIR
HHHHHHCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEE
IWTGELNLWLDKVTVCQYGNAPALDKKSLYCSIHFVIGFSKTCYRSLVDIEVGDVLLISN
EEECCEEEEEEEEEEEECCCCCCCCCCCEEEEEEEEEECCHHHHHHHHEEECCCEEEEEC
NLAYAVIYNTKIFDLIYPEELKMADHFEYEEDFETDDFDIKKNESEIYDENDDQMINSFE
CEEEEEEECCEEEEEECCHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHC
DLPVKIEFVLGKKIMNLYEIDELCAKRIISLLSESEKNIEIRVNGALTGYGELVEVDDKL
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEECCCC
GVEIHSWLSGHNNVK
CCCHHHHHCCCCCCC
>Mature Secondary Structure
MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAEESGSWSGWVLLGEIFP
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEHHHHHH
LISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRLRVGKVFDGVLSSAQPLIR
HHHHHHCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEE
IWTGELNLWLDKVTVCQYGNAPALDKKSLYCSIHFVIGFSKTCYRSLVDIEVGDVLLISN
EEECCEEEEEEEEEEEECCCCCCCCCCCEEEEEEEEEECCHHHHHHHHEEECCCEEEEEC
NLAYAVIYNTKIFDLIYPEELKMADHFEYEEDFETDDFDIKKNESEIYDENDDQMINSFE
CEEEEEEECCEEEEEECCHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHC
DLPVKIEFVLGKKIMNLYEIDELCAKRIISLLSESEKNIEIRVNGALTGYGELVEVDDKL
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEECCCC
GVEIHSWLSGHNNVK
CCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7638176 [H]