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The map label for this gene is yfiC [H]

Identifier: 157162055

GI number: 157162055

Start: 2750363

End: 2751100

Strand: Reverse

Name: yfiC [H]

Synonym: EcHS_A2731

Alternate gene names: 157162055

Gene position: 2751100-2750363 (Counterclockwise)

Preceding gene: 157162058

Following gene: 157162053

Centisome position: 59.25

GC content: 52.44

Gene sequence:

>738_bases
ATGTCACAGTCTACATCCGTGCTTCGTCGTAATGGATTTACTTTTAAACAGTTTTTTGTTGCTCACGATCGCTGTGCGAT
GAAAGTGGGAACGGATGGTATTTTATTGGGCGCATGGGCACCGGTGGCTGGGGTAAAACGTTGCCTTGATATCGGCGCGG
GTAGCGGGTTGCTGGCATTAATGCTGGCGCAGCGAACCGATGACAGCGTGATGATTGATGCAGTTGAACTGGAAAGTGAA
GCTGCCGCGCAGGCGCAGGAAAATATCAACCAGTCCCCGTGGGCAGAGCGGATTAACGTCCATACGGCGGATATTCAGCA
GTGGATCACACAGCAGACAGTACGCTTCGATTTAATCATCAGTAACCCACCTTACTATCAGCAGGGAGTAGAGTGCTCGA
CACCTCAACGGGAACAGGCTCGCTATACCACTACGCTTGATCACCCATCGTTGCTGACCTGTGCGGCGGAGTGCATTACC
GAAGAGGGATTTTTCTGCGTAGTGCTGCCAGAGCAGATTGGTAATGGTTTTACGGAGCTGGCATTAAGCATGGGCTGGCA
TTTACGTTTGCGGACGGATGTGGCGGAAAACGAAGCGCGACTGCCGCATCGGGTGCTGCTGGCATTCTCCCCGCAGGCGG
GAGAATGCTTTAGCGATCGCTTAGTCATTCGTGGGCCAGACCAGAACTATTCCGAAGCGTATACGGCGCTGACCCAGGCT
TTTTATCTGTTTATGTAA

Upstream 100 bases:

>100_bases
ACGTTACGGCTGCAATCTTCATCTGTTTGTATGGATATCGCTTCTCGGGTATTGTTCTCACCCGGTGCTATCGCCGTTTT
TCCTGCAAGGTTTTATTTTC

Downstream 100 bases:

>100_bases
TGATTGCCGGGGGAAAGGATCGACGGACCGGAATGCGTGAGCAGTTCCGGGTAATCCAGCGTAAAATGCAGGCCACGACT
CTCCTTGCGCATCATCGCAC

Product: putative methyltransferase

Products: NA

Alternate protein names: tRNA m6A37 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESE
AAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
FYLFM

Sequences:

>Translated_245_residues
MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESE
AAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
FYLFM
>Mature_244_residues
SQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEA
AAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECITE
EGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQAF
YLFM

Specific function: Specifically methylates the adenine in position 37 of tRNA(Val) [H]

COG id: COG4123

COG function: function code R; Predicted O-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. tRNA (adenine-N(6)-)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082131, Length=245, Percent_Identity=100, Blast_Score=512, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR007848
- InterPro:   IPR022882 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.55 [H]

Molecular weight: Translated: 27270; Mature: 27139

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLAL
CCCCHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEECCCCHHHHHHHHHCCCCCHHHHH
MLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
HHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHEEEEEEE
SNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTEL
CCCCHHHCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHH
ALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
HHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
FYLFM
HHHCC
>Mature Secondary Structure 
SQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAGVKRCLDIGAGSGLLAL
CCCHHHHHHCCCHHHHHHHHHHHEEEEECCCCEEEECCCCHHHHHHHHHCCCCCHHHHH
MLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHTADIQQWITQQTVRFDLII
HHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHEEEEEEE
SNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECITEEGFFCVVLPEQIGNGFTEL
CCCCHHHCCCCCCCCCHHHCCEEEECCCCHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHH
ALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFSDRLVIRGPDQNYSEAYTALTQA
HHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
FYLFM
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA