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The map label for this gene is yfiE

Identifier: 157162058

GI number: 157162058

Start: 2754054

End: 2754935

Strand: Reverse

Name: yfiE

Synonym: EcHS_A2734

Alternate gene names: 157162058

Gene position: 2754935-2754054 (Counterclockwise)

Preceding gene: 157162060

Following gene: 157162055

Centisome position: 59.33

GC content: 49.66

Gene sequence:

>882_bases
ATGGATCTGCGCCGTTTTATTACGCTTAAAACCGTGGTGGAAGAGGGTTCCTTTTTGCGAGCTTCGCAAAAATTGTGCTG
TACACAATCGACGGTGACTTTTCATATTCAGCAGCTTGAGCAGGAGTTTTCAGTCCAGTTATTTGAGAAAATTGGCCGAC
GGATGTGCCTTACCCGCGAAGGAAAAAAGTTGCTGCCGCACATTTATGAACTTACCCGAGTGATGGATACGCTTCGTGAG
GCTGCCAAAAAGGAGTCAGATCCGGACGGGGAACTACGCGTTGTTTCGGGCGAAACGTTGCTCTCTTACCGAATGCCGCA
GGTGTTGCAGCGTTTTCGGCAACGCGCCCCAAAAGTGCGTTTATCGTTGCAGTCGCTGAACTGTTACGTGATCCGTGATG
CGCTACTGAATGATGAGGCCGATGTTGGCGTCTTTTATCGTGTAGGGAATGATGATGCTCTGAATCGACGAGAGTTGGGT
GAACAATCACTGGTGCTGGTGGCTTCACCGCAAATTGCAGATGTCGATTTTACTGAGCCGGGAAGACATAACGCCTGTAG
CTTTATTATCAACGAACCGCAATGTGTCTTCCGGCAGATATTTGAGAGCACGCTGCGTCAGCGGCGGATCACGGTGGAAA
ACACCATTGAGCTTATAAGTATCGAAAGCATCAAGCGTTGTGTCGCGGCGAATATCGGGGTCAGCTACCTACCGCGCTTT
GCGGTGGCAAAAGAGTTGGAGTGCGGAGAATTAATTGAATTGCCTTTTGGCGAACAGTCGCAGACCATTACGGCCATGTG
CGCACATCATGCTGGAAAAGCGGTTAGCCCGGCGATGCACACTTTTATTCAGTGTGTTGAAGAGAGTTTTGTGGCTGGAT
AA

Upstream 100 bases:

>100_bases
GTTATCTCCTCTGTTTTGGTAGACAGTACCCGACACGGAACATTTAAAAAAATGAATGTTTTTATATTCTAGATTCGATA
TTTTCGATAGGTTTGGGGTT

Downstream 100 bases:

>100_bases
AAATAATGTCGGGATTAAACCCGGCATTTAACTTGTCGACAAAGTGCGCTTTATTCATGCCGGATGCGGCGTGAACGCCT
TATCCGGCCTACAAGATCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
AVAKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG

Sequences:

>Translated_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
AVAKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG
>Mature_293_residues
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLRE
AAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
AVAKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI87082132, Length=293, Percent_Identity=100, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=25.8503401360544, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=24.0418118466899, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786401, Length=313, Percent_Identity=26.8370607028754, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=30.625, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=21.8106995884774, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=22.9681978798587, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788706, Length=284, Percent_Identity=25.3521126760563, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082024, Length=271, Percent_Identity=25.830258302583, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=137, Percent_Identity=34.3065693430657, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=299, Percent_Identity=24.7491638795987, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YFIE_ECOLI (P33634)

Other databases:

- EMBL:   D13169
- EMBL:   D64044
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H65035
- RefSeq:   AP_003163.1
- RefSeq:   NP_417072.4
- ProteinModelPortal:   P33634
- STRING:   P33634
- EnsemblBacteria:   EBESCT00000002013
- EnsemblBacteria:   EBESCT00000015507
- GeneID:   947064
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2561
- KEGG:   eco:b2577
- EchoBASE:   EB1733
- EcoGene:   EG11785
- eggNOG:   COG0583
- GeneTree:   EBGT00070000031692
- HOGENOM:   HBG685425
- OMA:   VKELECG
- ProtClustDB:   CLSK880438
- BioCyc:   EcoCyc:EG11785-MONOMER
- Genevestigator:   P33634
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33244; Mature: 33244

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEE
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLVLVASPQIADVDFTEP
EEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEEEEECCCCCCCCCCCC
GRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AVAKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG
HHHHCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTRE
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEE
LSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLVLVASPQIADVDFTEP
EEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEEEEECCCCCCCCCCCC
GRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELISIESIKRCVAANIGVSYLPRF
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AVAKELECGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCVEESFVAG
HHHHCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837