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The map label for this gene is maeB

Identifier: 157161922

GI number: 157161922

Start: 2598005

End: 2600284

Strand: Reverse

Name: maeB

Synonym: EcHS_A2592

Alternate gene names: 157161922

Gene position: 2600284-2598005 (Counterclockwise)

Preceding gene: 157161925

Following gene: 157161921

Centisome position: 56.0

GC content: 54.17

Gene sequence:

>2280_bases
ATGGATGACCAGTTAAAACAAAGTGCACTTGATTTCCATGAATTTCCAGTTCCAGGGAAAATCCAGGTTTCTCCAACCAA
GCCTCTGGCAACACAGCGCGATCTGGCGCTGGCCTACTCACCAGGCGTTGCCGCACCTTGTCTTGAAATCGAAAAAGACC
CGTTAAAAGCCTACAAATATACCGCCCGCGGTAACCTGGTGGCGGTGATCTCTAACGGTACGGCGGTGCTGGGGTTAGGC
AACATTGGCGCGCTGGCAGGCAAACCGGTGATGGAAGGCAAGGGCGTTCTGTTTAAGAAATTCGCCGGGATTGATGTATT
TGACATTGAAGTTGACGAACTCGACCCGGACAAATTTATTGAAGTTGTCGCCGCGCTCGAACCAACCTTCGGCGGCATCA
ACCTCGAAGACATTAAAGCGCCAGAATGTTTCTATATTGAACAGAAACTGCGCGAGCGGATGAATATTCCGGTATTCCAC
GACGATCAGCACGGCACGGCAATTATCAGCACTGCCGCCATCCTCAACGGCTTGCGCGTGGTGGAGAAAAACATCTCCGA
CGTGCGGATGGTGGTTTCCGGCGCGGGTGCCGCAGCAATCGCCTGTATGAACCTGCTGGTAGCGCTGGGTCTGCAAAAAC
ATAACATCGTGGTTTGCGATTCAAAAGGCGTTATCTATCAGGGCCGTGAGCCAAACATGGCGGAAACCAAAGCCGCATAT
GCGGTGGTGGATGACGGCAAACGTACCCTCGATGATGTGATTGAAGGCGCGGATATTTTCCTGGGCTGTTCCGGCCCGAA
AGTGCTGACCCAGGAAATGGTGAAGAAAATGGCTCGTGCGCCAATGATCCTGGCGCTGGCGAACCCGGAACCGGAAATTC
TGCCGCCGCTGGCGAAAGAAGTGCGTCCGGATGCCATCATTTGCACCGGTCGTTCTGACTATCCGAACCAGGTGAACAAC
GTCCTGTGCTTCCCGTTCATCTTCCGTGGCGCGCTGGACGTTGGCGCAACCGCCATCAACGAAGAGATGAAACTGGCGGC
GGTACGTGCGATTGCAGAACTCGCCCATGCGGAACAGAGCGAAGTGGTGGCTTCAGCGTATGGCGATCAGGATCTGAGCT
TTGGTCCGGAATACATCATTCCAAAACCGTTTGATCCGCGCTTGATCGTTAAGATCGCTCCTGCGGTCGCTAAAGCCGCG
ATGGAGTCGGGCGTGGCGACTCGTCCGATTGCTGATTTCGACGTCTACATCGACAAGCTGACTGAGTTCGTTTACAAAAC
CAACCTGTTTATGAAGCCGATTTTCTCCCAGGCTCGCAAAGCGCCGAAGCGCGTTGTTCTGCCGGAAGGGGAAGAGGCGC
GCGTTCTGCATGCCACTCAGGAACTGGTAACGCTGGGACTGGCGAAACCGATCCTTATCGGTCGTCCGAACGTGATCGAA
ATGCGCATTCAGAAACTGGGCTTGCAGATCAAAGCGGGCGTTGATTTTGAGATCGTCAATAACGAATCCGATCCGCGCTT
TAAAGAGTACTGGACCGAATACTTCCAGATCATGAAGCGTCGCGGCGTCACTCAGGAACAGGCGCAGCGGGCGCTGATCA
GTAACCCGACAGTGATCGGCGCGATCATGGTTCAGCGTGGGGAAGCCGATGCAATGATTTGCGGTACGGTGGGTGATTAT
CATGAACATTTTAGCGTGGTGAAAAATGTCTTTGGTTATCGCGATGGCGTTCACACCGCAGGTGCCATGAACGCGCTGCT
GCTGCCGAGTGGTAACACCTTTATTGCCGATACATATGTTAATGATGAACCGGATGCAGAAGAGCTGGCGGAGATCACCT
TGATGGCGGCAGAAACTGTCCGTCGTTTTGGTATTGAGCCGCGCGTTGCTTTGTTGTCGCACTCCAACTTTGGTTCTTCT
GACTGCCCGTCGTCGAGCAAAATGCGTCAGGCGCTGGAACTGGTCAGGGAACGTGCACCAGAACTGATGATTGATGGTGA
AATGCACGGCGATGCAGCGCTGGTGGAAGCGATTCGCAACGACCGTATGCCGGACAGCTCTTTGAAAGGTTCCGCCAATA
TTCTGGTGATGCCGAACATGGAAGCTGCCCGCATTAGTTACAACTTACTGCGTGTTTCCAGCTCGGAAGGTGTGACTGTC
GGCCCGGTGCTGATGGGTGTGGCGAAACCGGTTCACGTGTTAACGCCGATCGCATCGGTGCGTCGTATCGTCAACATGGT
GGCGCTGGCCGTGGTAGAAGCGCAAACCCAACCGCTGTAA

Upstream 100 bases:

>100_bases
GTCTGTAGACTCCGGCAGGGTAATAATGTGCGCCACGTTGTGGGCAGGGGCTGTTGCCCACACACTTTATTTGTGAACGT
TACGTGAAAGGAACAACCAA

Downstream 100 bases:

>100_bases
TTTTTTTTAACTCTCACGCTTACCCTGAATATTCAGGGTAAGCAGTTTAGCTGCAATATATTAGTAAAGCTTATTACTGA
GTTTGCGAATAATAAAAAAA

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME

Number of amino acids: Translated: 759; Mature: 759

Protein sequence:

>759_residues
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLG
NIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNN
VLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
MESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDY
HEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSS
DCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDRMPDSSLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL

Sequences:

>Translated_759_residues
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLG
NIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNN
VLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
MESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDY
HEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSS
DCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDRMPDSSLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL
>Mature_759_residues
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLG
NIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNN
VLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAA
MESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDY
HEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSS
DCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDRMPDSSLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family

Homologues:

Organism=Homo sapiens, GI4505143, Length=402, Percent_Identity=25.8706467661692, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI62420882, Length=345, Percent_Identity=26.3768115942029, Blast_Score=77, Evalue=9e-14,
Organism=Homo sapiens, GI62420880, Length=345, Percent_Identity=26.3768115942029, Blast_Score=77, Evalue=9e-14,
Organism=Homo sapiens, GI239049447, Length=345, Percent_Identity=26.3768115942029, Blast_Score=77, Evalue=9e-14,
Organism=Escherichia coli, GI1788806, Length=759, Percent_Identity=100, Blast_Score=1548, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=349, Percent_Identity=31.2320916905444, Blast_Score=166, Evalue=7e-42,
Organism=Escherichia coli, GI1788800, Length=326, Percent_Identity=34.3558282208589, Blast_Score=163, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17537199, Length=398, Percent_Identity=25.8793969849246, Blast_Score=66, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=268, Percent_Identity=29.8507462686567, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24646388, Length=346, Percent_Identity=28.0346820809249, Blast_Score=83, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24646386, Length=346, Percent_Identity=28.0346820809249, Blast_Score=83, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281362672, Length=396, Percent_Identity=25, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281362674, Length=396, Percent_Identity=25, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356279, Length=396, Percent_Identity=25, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAO2_ECOLI (P76558)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F65021
- RefSeq:   AP_003048.1
- RefSeq:   NP_416958.1
- ProteinModelPortal:   P76558
- DIP:   DIP-10141N
- IntAct:   P76558
- STRING:   P76558
- PRIDE:   P76558
- EnsemblBacteria:   EBESCT00000001945
- EnsemblBacteria:   EBESCT00000017060
- GeneID:   946947
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2447
- KEGG:   eco:b2463
- EchoBASE:   EB3945
- EcoGene:   EG14193
- eggNOG:   COG0281
- GeneTree:   EBGT00050000008963
- HOGENOM:   HBG289132
- OMA:   IELAEND
- ProtClustDB:   PRK07232
- BioCyc:   EcoCyc:MALIC-NADP-MONOMER
- BioCyc:   MetaCyc:MALIC-NADP-MONOMER
- Genevestigator:   P76558
- GO:   GO:0005829
- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF036684
- SMART:   SM00919

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB

EC number: =1.1.1.40

Molecular weight: Translated: 82418; Mature: 82418

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: ACT_SITE 94-94 BINDING 162-162 BINDING 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKY
CCCHHHHHHCCHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCHHCCCCCHHHEEE
TARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFI
ECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEHCCCCEEEEEEECCCCHHHHH
EVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAILNGLRV
HHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
VEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHEEE
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKE
EEECCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
VRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQS
CCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHH
EVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMESGVATRPIADFDVYIDKL
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHH
TEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
HHHHHHHCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHH
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIG
HHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
AIMVQRGEADAMICGTVGDYHEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYV
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECC
NDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
ELMIDGEMHGDAALVEAIRNDRMPDSSLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
CEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEEEEEEECCCCCEE
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKY
CCCHHHHHHCCHHCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCHHCCCCCHHHEEE
TARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFI
ECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCEEEEHCCCCEEEEEEECCCCHHHHH
EVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAILNGLRV
HHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
VEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY
HHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHEEE
AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKE
EEECCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
VRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQS
CCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCHHH
EVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMESGVATRPIADFDVYIDKL
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHH
TEFVYKTNLFMKPIFSQARKAPKRVVLPEGEEARVLHATQELVTLGLAKPILIGRPNVIE
HHHHHHHCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHH
MRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYFQIMKRRGVTQEQAQRALISNPTVIG
HHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE
AIMVQRGEADAMICGTVGDYHEHFSVVKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYV
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEECC
NDEPDAEELAEITLMAAETVRRFGIEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
ELMIDGEMHGDAALVEAIRNDRMPDSSLKGSANILVMPNMEAARISYNLLRVSSSEGVTV
CEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHEEEEEEEEECCCCCEE
GPVLMGVAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376