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The map label for this gene is yfbT

Identifier: 157161781

GI number: 157161781

Start: 2453010

End: 2453660

Strand: Reverse

Name: yfbT

Synonym: EcHS_A2442

Alternate gene names: 157161781

Gene position: 2453660-2453010 (Counterclockwise)

Preceding gene: 157161782

Following gene: 157161780

Centisome position: 52.84

GC content: 56.53

Gene sequence:

>651_bases
GTGCGGTGCAAAGGTTTTCTGTTTGATCTTGATGGAACGCTGGTGGATTCCCTGCCTGCGGTAGAACGGGCGTGGAGCAA
CTGGGCCAGACGTCATGGGTTAGCGCCGGAAGAGGTGCTGGCTTTCATTCACGGTAAACAGGCGATCACCTCTCTGCGCC
ATTTTATGGCGGGCAAATCCGAGGCTGATATTGCCGCCGAGTTTACGCGTCTGGAGCACATCGAGGCCACGGAAACCGAA
GGTATTACCGCGCTTCCGGGGGCAATCGCCTTACTCAGTCATTTGAATAAAGCAGGTATTCCGTGGGCCATTGTGACTTC
TGGCTCCATGCCGGTAGCGCGAGCGCGCCATAAAATAGCTGGGCTTCCCGCACCAGAGGTGTTTGTAACCGCTGAGCGAG
TGAAGCGCGGAAAACCAGAACCTGATGCGTATCTGTTAGGCGCGCAGCTGCTGGGGCTTGCGCCGCAGGAGTGTGTGGTG
GTGGAAGATGCTCCCGCTGGCGTGCTTTCTGGCCTGGCGGCGGGTTGTCATGTCATTGCGGTTAACGCTCCGGCAGATAC
CCCGCGCCTGAATGAGGTCGATTTGGTCCTCCACAGTCTGGAGCAAATTACTGTGACCAAACAGCCAAATGGCGATGTTA
TTATTCAGTGA

Upstream 100 bases:

>100_bases
AAATATCAGCGCATGCTTAATGTGTGGCATGCCTGCCCGCGTCAGTACCATTTGAGCGCCAACGAAATTAATCAAATTAT
CAATGCCTGAGGAGGCCCGC

Downstream 100 bases:

>100_bases
AACACCTGATCATGATTTAGCCCCGTCTCGTCGGGGCTTTTTTATGGCAGAATCAAGTCATCCCCCTCAATTAACAAGGA
TAAGTTGTGAACGGTGAATT

Product: putative phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETE
GITALPGAIALLSHLNKAGIPWAIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ

Sequences:

>Translated_216_residues
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETE
GITALPGAIALLSHLNKAGIPWAIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ
>Mature_216_residues
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETE
GITALPGAIALLSHLNKAGIPWAIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family

Homologues:

Organism=Homo sapiens, GI197382691, Length=212, Percent_Identity=30.188679245283, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI207113149, Length=231, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI87082080, Length=216, Percent_Identity=100, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI1790151, Length=202, Percent_Identity=31.1881188118812, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789046, Length=192, Percent_Identity=30.2083333333333, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321834, Length=229, Percent_Identity=32.3144104803493, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321833, Length=229, Percent_Identity=31.8777292576419, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322136, Length=225, Percent_Identity=31.1111111111111, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320905, Length=225, Percent_Identity=31.1111111111111, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129151, Length=195, Percent_Identity=28.7179487179487, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFBT_ECOLI (P77625)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C65001
- RefSeq:   AP_002893.1
- RefSeq:   NP_416796.2
- ProteinModelPortal:   P77625
- SMR:   P77625
- DIP:   DIP-11972N
- MINT:   MINT-1275778
- STRING:   P77625
- EnsemblBacteria:   EBESCT00000000977
- EnsemblBacteria:   EBESCT00000018371
- GeneID:   946777
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5376
- KEGG:   eco:b2293
- EchoBASE:   EB3857
- EcoGene:   EG14104
- eggNOG:   COG0637
- GeneTree:   EBGT00050000009692
- HOGENOM:   HBG742904
- OMA:   VVERSWC
- ProtClustDB:   PRK11587
- BioCyc:   EcoCyc:G7187-MONOMER
- BioCyc:   MetaCyc:G7187-MONOMER
- Genevestigator:   P77625
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:1.10.150.240
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: NA

Molecular weight: Translated: 23008; Mature: 23008

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: ACT_SITE 9-9

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKS
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EADIAAEFTRLEHIEATETEGITALPGAIALLSHLNKAGIPWAIVTSGSMPVARARHKIA
CHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHC
GLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVVVEDAPAGVLSGLAAGCHVIA
CCCCCHHEEEHHHHHCCCCCCCCEEHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCEEEE
VNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ
EECCCCCCCCCHHHHHHHHHHHEEEEECCCCCEEEC
>Mature Secondary Structure
MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIHGKQAITSLRHFMAGKS
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EADIAAEFTRLEHIEATETEGITALPGAIALLSHLNKAGIPWAIVTSGSMPVARARHKIA
CHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHC
GLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVVVEDAPAGVLSGLAAGCHVIA
CCCCCHHEEEHHHHHCCCCCCCCEEHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCEEEE
VNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ
EECCCCCCCCCHHHHHHHHHHHEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123